Enhanced sensitivity RNA gel loading buffer that enables efficient RNA separation on native gels

BioTechniques

Volumn 36, Issue 2, 2004, Pages 334-336

  Gregg, Keqin ^a   Zhou, Wenli ^a   Ji, Wan ^a   Davis, Sara ^a  

^a Viagen Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; ELECTROPHORESIS; GELS; GENES; HAZARDOUS MATERIALS; SEPARATION;

GENE EXPRESSION; MICROARRAYS;

RNA;

AGAROSE; BUFFER; EDETIC ACID; ETHIDIUM BROMIDE; FORMALDEHYDE; RIBOSOME RNA; RNA; RNA GEL LOADING BUFFER; SUPERLOAD BUFFER; TROMETAMOL; UNCLASSIFIED DRUG;

AGAR GEL ELECTROPHORESIS; ANALYTIC METHOD; ANIMAL CELL; DANGEROUS GOODS; DNA FINGERPRINTING; DNA MICROARRAY; ELECTROPHORESIS; GEL; GEL ELECTROPHORESIS; GENE EXPRESSION REGULATION; NONHUMAN; QUALITY CONTROL; QUANTITATIVE ASSAY; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVIEW; RNA ANALYSIS; RNA ISOLATION; SEPARATION TECHNIQUE; STANDARD; TIME; WORK;

ANIMALIA;

EID: 0842309818     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04362pf01     Document Type: Review

References (5)

1. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Electrophoresis of RNA through gels containing formaldehyde, p. 7.43-7.45. In Molecular Cloning, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
2. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Agarose gel electrophoresis, p, 6.3-6.19. In Molecular Cloning, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
3. Wang, J., L. Hu, S.R. Hamilton, K.R. Coombes, and W. Zhang. 2003. RNA amplification strategies for cDNA microarray experiments. BioTechniques 34:394-400.
4. Bustin, S.A. 2000. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25:169-193.
5. Gauthier, E.R., S.D. Madison, and R.N. Michel. 1997. Rapid RNA isolation without the use of commercial kits: application to small tissue samples. Eur. J. Physiol. 433:664-668.