Fidelity of targeting to chloroplasts is not affected by removal of the phosphorylation site from the transit peptide

European Journal of Biochemistry

Volumn 271, Issue 3, 2004, Pages 509-516

  Nakrieko, Kerry Ann ^a   Mould, Ruth M ^a   Smith, Alison G ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Amino acyl tRNA synthetase; Confocal microscopy; Dual targeting; GFP; Rubisco

Indexed keywords

AMINO ACID TRANSFER RNA LIGASE; CYSTEINE TRANSFER RNA LIGASE; GREEN FLUORESCENT PROTEIN; HEAT SHOCK PROTEIN 70; HISTIDINE TRANSFER RNA LIGASE; PEPTIDE DERIVATIVE; PROTEIN 14 3 3; PROTEIN SUBUNIT; RIBULOSEBISPHOSPHATE CARBOXYLASE; UNCLASSIFIED DRUG;

ARTICLE; CELL ORGANELLE; CHLOROPLAST; GENE TARGETING; IN VIVO STUDY; MITOCHONDRION; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; PRIORITY JOURNAL;

CHLOROPLASTS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; PEAS; PEPTIDES; PHOSPHORYLATION; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS;

EID: 0842287441     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03950.x     Document Type: Article

References (33)

1. Jarvis, P. & Soll, J. (2001) Toc, Tic, and chloroplast protein import. Biochim. Biophys. Acta 1541, 64-79.
2. Glaser, E., Sjoling, S., Tanudji, M. & Whelan, J. (1998) Mitochondrial protein import in plants - signals, sorting, targeting, processing and regulation. Plant Mol. Biol. 38, 311-338.
3. Zhang, X.P. & Glaser, E. (2002) Interaction of plant mitochondrial and chloroplast signal peptides with the Hsp70 molecular chaperone. Trends Plant Sci. 7, 14-21.
4. von Heijne, G. & Niskikawa, K. (1991) Chloroplast transit peptides: the perfect random coil? FEBS Lett. 278, 1-3.
5. Emanuelsson, O. & von Heijne, G. (2001) Prediction of organellar targeting signals. Biochim. Biophys. Acta 1541, 114-119.
6. Kubo, N., Arimura, S., Tsutsumi, N., Hirai, A. & Kadowaki, K. (2003) Involvement of N-terminal region in mitochondrial targeting office RPS10 and RPS14 proteins. Plant Sci. 164, 1047-1055.
7. Chaumont, F., O'Riordan, V. & Boutry, M. (1990) Protein transport into mitochondria is conserved between plant and yeast species. J. Biol. Chem. 265, 16856-16862.
8. Whelan, J., Knorpp, C., Harmey, M.A. & Glaser, E. (1991) Specificity of leaf mitochondrial and chloroplast processing systems for nuclear-encoded precursor proteins. Plant Mol. Biol. 16, 283-292.
9. Boutry, M., Nagy, F., Poulsen, C., Aoyagi, K. & Chua, N.H. (1987) Targeting of bacterial chloramphenicol acetyltransferase to mitochondria in transgenic plants. Nature 328, 340-342.
10. Duchene, A.M., Peeters, N., Dietrich, A., Cosset, A., Small, I.D. & Wintz, H. (2001) Overlapping destinations for two dual targeted glycyl-tRNA synthetases in Arabidopsis thaliana and Phaseolus vulgaris. J. Biol. Chem. 276, 15275-15283.
11. Peeters, N.M., Chapron, A., Giritch, A., Grandjean, O., Lancelin, D., Lhomme, T., Vivrel, A. & Small, I. (2000) Duplication and quadruplication of Arabidopsis thaliana cysteinyl-and asparaginyl-tRNA synthetase genes of organellar origin. J. Mol. Evol. 50, 413-423.
12. Akashi, K., Grandjean, O. & Small, I. (1998) Potential dual targeting of an Arabidopsis archaebacterial-like histidyl-tRNA synthetase to mitochondria and chloroplasts. FEBS Lett. 431, 39-14.
13. Waegemann, K. & Soll, J. (1996) Phosphorylation of the transit sequence of chloroplast precursor proteins. J. Biol. Chem. 271, 6545-6554.
14. May, T. & Soll, J. (2000) 14-3-3 proteins form a guidance complex with chloroplast precursor proteins in plants. Plant Cell 12, 53-63.
15. Davis, S.J. & Vierstra, R.D. (1998) Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol. Biol. 36, 521-528.
16. Mazur, B.J. & Chui, C.F. (1985) Sequence of a genomic DNA clone for the small subunit of ribulose bis-phosphate carboxylase-oxygenase from tobacco. Nucleic Acids Res. 13, 2373-2386.
17. Sheen, J., Hwang, S.B., Niwa, Y., Kobayashi, H. & Galbraith, D.W. (1995) Green fluorescent protein as a new vital marker in plant cells. Plant J. 8, 777-784.
18. Anderson, S. & Smith, S.M. (1986) Synthesis of the small subunit of ribulose bisphosphate carboxylase from genes cloned into plasmids containing the SP6 promoter. Biochem. J. 240, 709-715.
19. Cleary, S.P., Tan, F.C., Nakrieko, K.A., Thompson, S.J., Mullineaux, P.M., Creissen, G.P., von Stedingk, E., Glaser, E., Smith, A.G. & Robinson, C. (2002) Isolated plant mitochondria import chloroplast precursor proteins in vitro with the same efficiency as chloroplasts. J. Biol. Chem. 277, 5562-5569.
20. Gray, J.C., Sullivan, J.A., Hibberd, J.M. & Hansen, M.R. (2001) Stromules: mobile protrusions and interconnections between plastids. Plant Biol. 3, 223-233.
21. Mihara, K. & Omura, T. (1996) Cytoplasmic chaperones in precursor targeting to mitochondria: the role of MSF and hsp70. Trends Cell Biol. 6, 104-108.
22. Alam, R., Hachiyan, N., Sakaguchi, M., Kawabata, S., Iwanaga, S., Kitajima, M., Mihara, K. & Omura, T. (1994) cDNA cloning and characterization of mitochondrial import stimulation factor (MSF) purified from rat liver cytosol. J. Biochem. 116, 416-425.
23. Murakami, H., Pain, D. & Blobel, G. (1988) 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating import into mitochondria. J. Cell Biol. 107, 2051-2057.
24. Iwahashi, J., Furuya, S., Mihara, K. & Omura, T. (1992) Characterization of adrenodoxin precursor expressed in E. coli. J. Biochem. 111, 451-455.
25. Wienk, H.L.J., Czisch, M. & de Kruijff, B. (1999) The structural flexibility of the preferredoxin transit peptide. FEBS Lett. 453, 318-326.
26. Chow, K.S., Singh, D.P., Walker, A.R. & Smith, A.G. (1998) Two different genes encode ferrochelatase in Arabidopsis: mapping, expression and subcellular targeting of the precursor proteins. Plant J. 15, 531-541.
27. Suzuki, T., Masuda, T., Singh, D.P., Tan, F.C., Tsuchiya, T., Shimada, H., Ohta, H., Smith, A.G. & Takamiya, K. (2002) Two types of ferrochelatase in photosynthetic and non-photosynthetic tissues of cucumber; their difference in phylogeny, gene expression and localization. J. Biol. Chem. 277, 4731-4737.
28. Chow, K.S., Singh, D.P., Roper, J.M. & Smith, A.G. (1997) A single precursor protein for ferrochelatase-I from Arabidopsis is imported in vitro to both chloroplasts and mitochondria. J. Biol. Chem. 272, 27565-27571.
29. Lister, R., Chew, O., Rudhe, C., Lee, M.N. & Whelan, J. (2001) Arabidopsis thaliana ferrochelatase-I and -II are not imported into Arabidopsis mitochondria. FEBS Lett. 506, 291-295.
30. Rudhe, C., Chew, O., Whelan, J. & Glaser, E. (2002) A novel in vitro system for simultaneous import of precursor proteins into mitochondria and chloroplasts. Plant J. 30, 213-220.
31. Chen, L.J. & Li, H.M. (1998) A mutant deficient in the plastid lipid DGD is defective in protein import into chloroplasts. Plant J. 16, 33-39.
32. Ellis, E.M. & Reid, G.A. (1993) The Saccharomyces cervisiae mts1 gene encodes a putative RNA-binding protein involved in mitochondrial protein targeting. Gene 132, 175-183.
33. Macasev, D., Newbigin, E., Whelan, J. & Lithgow, T. (2000) How do plant mitochondria avoid importing chloroplast proteins? Components of the import apparatus Tom20 and Tom22 from Arabidopsis differ from their fungal counterparts. Plant Physiol. 123, 811-816.