Recognition and Separation of Single Particles with Size Variation by Statistical Analysis of their Images

Journal of Molecular Biology

Volumn 336, Issue 2, 2004, Pages 453-460

  White, Helen E ^a   Saibil, Helen R ^a   Ignatiou, Athanasios ^a   Orlova, Elena V ^a  

^a UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Cryo electron microscopy; Heat shock protein; Heterogeneity; Multivariate statistical analysis; Single particle reconstruction

Indexed keywords

HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 26; PROTEIN; PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL STRUCTURE; CRYOELECTRON MICROSCOPY; DATA ANALYSIS; IMAGING; MODEL; MOLECULAR RECOGNITION; MULTIVARIATE ANALYSIS; PARTICLE SIZE; PARTICULATE MATTER; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; SIMULATION; STATISTICAL ANALYSIS; TECHNIQUE;

EID: 0742272143     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2003.12.015     Document Type: Article

References (23)

1. Orlova E.V., Rahman M.A., Gowen B., Volynski K.E., Ashton A.C., Manser C., et al. Structure of alpha-latrotoxin oligomers reveals that divalent cation-dependent tetramers form membrane pores. Nature Struct. Biol. 7:2000;48-53.
2. Roseman A.M., Ranson N.A., Gowen B., Fuller S.D., Saibil H.R. Structures of unliganded and ATP-bound states of the Escherichia coli chaperonin GroEL by cryoelectron microscopy. J. Struct. Biol. 135:2001;115-125.
3. Jimenez J.L., Nettleton E.J., Bouchard M., Robinson C.V., Dobson C.M., Saibil H.R. The protofilament structure of insulin amyloid fibrils. Proc. Natl Acad. Sci. USA. 99:2002;9196-9201.
4. Frank J. Averaging of low exposure electron micrographs of nonperiodic objects. Ultramicroscopy. 1:1975;159-162.
5. van Heel M., Gowen B., Matadeen R., Orlova E.V., Finn R., Pape T., et al. Single-particle electron cryo-microscopy: towards atomic resolution. Quart. Rev. Biophys. 33:2000;307-365.
6. Frank J., Verschoor A., Boublik M. Computer averaging of electron micrographs of 40 S ribosomal subunits. Science. 214:1981;1353-1355.
7. van Heel M., Frank J. Use of multivariate statistics in analysing the images of biological macromolecules. Ultramicroscopy. 6:1981;187-194.
8. van Heel M. Multivariate statistical classification of noisy images (randomly oriented biological macromolecules). Ultramicroscopy. 13:1984;165-183.
9. van Heel M. Classification of very large electron microscopical image data sets. Optik. 82:1989;114-126.
10. Frank J. Classification of macromolecular assemblies studied as "single particles" Quart. Rev. Biophys. 23:1990;281-329.
11. Dube P., Tavares P., Lurz R., van Heel M. The portal protein of bacteriophage SPP1: a DNA pump with 13-fold symmetry. EMBO J. 12:1993;1303-1309.
12. Staley J.P., Guthrie C. Mechanical devices of the spliceosome: motors, clocks, springs, and things. Cell. 92:1998;315-326.
13. Orlova E.V., Sherman M.B., Chiu W., Mowri H., Smith L.C., Gotto A.M. Jr. Three-dimensional structure of low density lipoproteins by electron cryomicroscopy. Proc. Natl Acad. Sci. USA. 96:1999;8420-8425.
14. Saibil H.R., Horwich A.L., Fenton W.A. Allostery and protein substrate conformational change during GroEL/GroES-mediated protein folding. Adv. Protein Chem. 59:2002;45-71.
15. Stark H. Three-dimensional electron cryomicroscopy of ribosomes. Curr. Protein Pept. Sci. 3:2002;79-91.
16. Serysheva I., Orlova E.V., Sherman M., Chiu W., Hamilton S., van Heel M. The skeletal muscle calcium-release channel in its closed state visualized by electron microscopy and angular reconstitution. Nature Struct. Biol. 2:1995;18-24.
17. Penczek P., Grassuci R.A., Frank J. The ribosome at improved resolution: new techniques for merging and orientation refinement in 3D cryoelectron microscopy of biological particles. Ultramicroscopy. 53:1994;251-270.
18. Frank J., van Heel M. Correspondence analysis of aligned images of biological particles. J. Mol. Biol. 161:1982;134-137.
19. Frank J. Three-dimensional Microscopy of Macromolecular Assemblies. 1996;Academic Press, San Diego.
20. van Heel M., Harauz G., Orlova E.V., Schmidt R., Schatz M. A new generation of the IMAGIC image processing system. J. Struct. Biol. 116:1996;17-24.
21. Frank J., Radermacher M., Penczek P., Zhu J., Li Y., Ladjadj M., Leith A. SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields. J. Struct. Biol. 116:1996;190-199.
22. Crowther R.A., Henderson R., Smith J.M. MRC image processing programs. J. Struct. Biol. 116:1996;9-16.
23. Harauz G., van Heel M. Exact filters for general geometry three dimensional reconstruction. Optik. 73:1986;146-156.