Extracellular proteins secreted in the culture medium of embryogenic and non-embryogenic cell suspensions of sugarcane (Saccharum sp. L.) cv 78-1;Proteinas extracelulares secretadas en el medio de cultivo de suspensiones celulares embriogenicas y no-embriogenicas de caña de azucar (Saccharum sp. L.) cv. V78-1

Acta Cientifica Venezolana

Volumn 49, Issue 3, 1998, Pages 160-165

  Esqueda, Maribel Colmenares ^a   Oropeza, Maira ^b   De García, Eva ^b  

^a FACULTAD DE CIENCIAS VETERINARIAS   (Venezuela)

^b FACULTAD DE CIENCIAS   (Venezuela)

Author keywords

Extracellular protein; Saccharum sp. L.; Somatic embryogenesis

Indexed keywords

EID: 0542445252     PISSN: 00015504     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Blakesly, R.W. and Boezi, J.A. A new staining technique for proteins in polyacrylamide gels using Coomassie Brillant Blue G250. Anal. Biochem. 82:580-582, 1977.
2. Bollag, D. and Edlstein, S. Protein Methods. Willey-Liss, Inc. New York, 1992, p. 230.
3. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254. 1976.
4. Carman, J. G. Embryogenic cells in plant tissue cultures: ocurrence and behavior. In Vitro Cell Dev. Biol. 20: 746-753, 1990.
5. Coutos-Thevenot, P., Jouenne, T., Maes, O., Guerbette, F., Grosbois, M., Le Caer, J.P., Boulay, M., Deloire, A., Kade,r J.C., and Guern, J. Four 9-kDa protein excreted by somatic embryos of grapevine are isoforms of lipid-transfer proteins. Eur. J. Biochem. 217: 885-889, 1993.
6. Coutos-Thevenot, P., Maes, O., Jouenne, T., Mauro, M.C., Boulay, M., Deloire, A., and Guern, J. Extracellular protein patterns of grapevine cell suspensions in embryogenic and non-embryogenic situations. Plant Sci. 86: 137-145, 1992.
7. De Jong, A.J., Cordewener, J., Lo Shiavo, F., Terzi, M., Vandekerckhove, J., Van Kammen, A. and De Vries, S.C. A carrot somatic embryo mutant is rescued by chitinase. The Plant Cell. 4: 425-433, 1992.
8. De Vries, S.C., Booij, H., Janssens, R., Vogel, R., Saris, L., Lo Shiavo, S., Terzi, M., and Van Kammen, A. Carrot somatic embryogenesis depends on the phytohormone controlled presence of correctly glycosylated extracellular proteins. Genes Dev. 2: 462-476, 1988a.
9. De Vries, S.C., Booij, H., Meyerink, P., Huisman, G., Wilde, D.H., Thomas T.L., and Van Kammen, A. Acquisition of embryogenic potential in carrot cell-suspension cultures. Planta 176: 196-204, 1988b.
10. Dong, J.Z. and Dunstan, D.I. Growth parameters, protein and DNA synthesis of an embryogenic suspension culture of White Spruce (Picea glauca). J. Plant Physiol. 144: 201-208, 1994.
11. Dudits D., Bogre, L., and Gyorgyey, J. Molecular and cellular approaches to the analysis of plant embryo development from somatic cells in vitro. J. Cell Sci. 99: 475-484, 1991.
12. García, E. Variaciones somaclonales y su aplicación en los estudios de mejoramiento de la caña de azúcar. I Curso de cultivo de tejidos vegetales aplicado a la producción agrícola. Caracas, Venezuela. Villegas, L. (Ed). CAF. Signo Comtemporáneo S.R.L. Caracas, 1988, p. 218.
13. Ho, W.J. and Vasil, I.K. Somatic embryogenesis in sugarcane (Saccharum officinarum L.) I The morphology and physiology of callus formation and the ontogeny of somatic embryos. Protoplasma 118: 169-180, 1983.
14. Ho, W.J. and Vasil, I.K. Somatic embryogenesis in sugarcane (Saccharum officinarum L.): Grow and plant regeneration from embryogenic cell suspension cultures. Ann. Bot. 51: 719-720, 1983.
15. Kreuger M. and Van Holst, G.J. Arabinogalactan proteins are essential in somatic embryogenesis of Daucus carota L. Planta. 189: 243-248, 1993.
16. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Plant Physiol. 81: 680-685, 1970.
17. Merril, C.R., Goldman, D., Sedman, S.A. and Ebert, M. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211: 1437-38, 1981.
18. Murashige, T. and Skoog, F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant. 15: 473-497, 1962.
19. Nielsen K.A. and Hansen, I.B. Appearance of extracellular proteins associated with somatic embryogenesis in suspension cultures of barley (Hordeum vulgare L.). J. Plant. Physiol. 139: 489-497, 1992.
20. Poulsen, G.B., Frugis, G., Albrechtsen, M. and Mariotti, D. Synthesis of extracellular proteins in embryogenic and non-embryogenic cell cultures of alfalfa. Plant Cell, Tissue and Organ Culture 44: 257-200, 1996.
21. Schmidt, D.L., de Jong, A.J. and de Vries, S.C. Signal molecules involved in plant embryogenesis. Plant Mol. Biol. 20: 1305-1313, 1994.
22. Williams, E.G. and Maheswaran, G. Somatic embryogenesis: factors influencing coordinated behaviour of cells as an embryogenic group. Annals of Botany 57: 443-462, 1986.
23. Zambrano, A.Y., Gonzalez, V. y Demey, J.R. Tiempo Optimo Para el Cambio en Suspensiones Celulares de Cuatro Cultivares deCaña de Azúcar Saccharum sp.: B6749, CP5659, V58-4 y V64-10. Agronomía Trop 2: 179-202, 1994.