A Method for Isolating Total RNA from Pear Leaves

Plant Molecular Biology Reporter

Volumn 19, Issue 1, 2001, Pages

  Malnoy, M ^a   Reynoird, J P ^a,b   Mourgues, F ^a,c   Chevreau, E ^a   Simoneau, P ^d  

^a CEMAGREF   (France)

^b Department of Obstetrics Gynecology   (United States)

^c Fruit Tree Research Station ISF   (Italy)

^d UNIVERSITÉ D'ANGERS   (France)

Author keywords

Pear; Polysaccharide; RNA contamination; RNA extraction

Indexed keywords

EID: 0442327929     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02824081     Document Type: Article

References (14)

1. Baker SS, Rugh CL and Kamalay JC (1990) RNA and DNA isolation from recalcitrant plant tissues. BioTechniques 9: 268-272.
2. Church GM and Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81: 1991-1995.
3. Guena F, Hartings H and Scienza A (1998) A new method for extraction of high quality RNA from recalcitrant tissues of grapevine. Plant Mol Biol Reptr 16: 61-67.
4. Lay-Yee M, Della-Penna D and Ross GS (1990) Changes in mRNA and protein during ripening of apple fruit (Malus domestica Borkh. cv. Golden Delicious). Plant Physiol 94: 850-853.
5. Levi A, Galau GA and Wetzstein HY (1992) A rapid procedure for the isolation of RNA from high-phenolic-containing tissues of pecan. Hort Sci 27: 1316-1318.
6. Logemann J, Schell J and Willmitzer L (1987) Improved method for the isolation of RNA from plant tissues. Anal Biochem 163: 16-20.
7. Lopez-Gomez R and Gomez-Lim MA (1992) A method for extraction of intact RNA from fruits rich in polysaccharides using ripe mango mesocarp. Hort Sci 27: 440-442.
8. Manning K (1990) Isolation of nucleic acids from plants by differential solvent precipitation. Anal. Biochem 195: 45-50.
9. Mitra D and Kootstra A (1993) Isolation of RNA from apple skin. Plant Mol Biol Reptr 11: 326-332.
10. Rosati C, Cadic A, Duron M, Renou JP and Simoneau P (1997) Molecular cloning and expression analysis of dihydroflavonol 4-reductase gene in flower organs of Forsythia X intermedia. Plant Mol Biol 35: 303-311.
11. Schultz DJ, Craig R, Cox-Foster DL, Mumma RO and Medford JO (1994) RNA isolation from recalcitrant plant tissue. Plant Mol Biol Reptr 12: 310-316.
12. Schneiderbauer A, Sandermann H Jr and Ernst D (1991) Isolation of functional RNA from plant tissues rich in phenolic compounds. Anal Biochem 197: 91-97.
13. Tesniere C and Vayda ME (1991) Method for isolation of high-quality RNA from grape berry tissues without contaminating tannins or carbohydrates. Plant Mol Biol Reptr 9: 242-251.
14. Wilson MF and Blunden CA (1983) Changes in the levels of polyphenolics in tree pear varieties during bud development. J Sci Food Agric 34: 973-978.