DNA Extraction from Plants: The Use of Pectinase

Plant Molecular Biology Reporter

Volumn 19, Issue 4, 2001, Pages 353-359

  Rogstad, Steven H ^a   Keane, Brian ^a   Keiffer, Carolyn Howes ^b   Hebard, Fred ^c   Sisco, Paul ^c  

^a UNIVERSITY OF CINCINNATI   (United States)

^b MIAMI UNIVERSITY   (United States)

^c American Chestnut Foundation   (United States)

Author keywords

DNA contamination removal; Pectin degradation; Plant DNA extraction; Plant RNA extraction; Polygalacturonase

Indexed keywords

EID: 0442280806     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02772833     Document Type: Article

References (11)

1. th Edition. Academic Press, New York, New York, USA.
2. Doyle JJ and Doyle JL (1987) A rapid DNA isolation from small amount of fresh leaf tissue. Phytochem Bull 19: 11-15.
3. Hillis DM, Larson A, Davis SK, and Zimmer E (1990) Nucleic acids III: sequencing. In: Hillis DM and Moritz C (eds), Molecular Systematics. Sinauer Associates, Sunderland, Massachusetts, USA.
4. Junowicz E and Spencer J (1973) Studies on bovine pancreatic deoxyribonuclase A. I. General properties and activation with different bivalent metals. Biochim Biophys Acta 312: 72-77.
5. Lazarides E and Lindberg U (1974) Actin is the naturally occurring inhibitor of deoxyribonuclease I. Proc Natl Acad Sci USA 71: 4742-4746.
6. Liao T (1975) Reversible inactivation of pancreatic deoxyribonuclease A by sodium dodecyl sulfate. J Biol Chem 250: 3831-3836.
7. Liu D and Wu R (1999) Protection of megabase-sized chromosomal DNA from breakage by DNAse activity in plant nuclei. Biotechniques 26: 258-261.
8. Okada M, Whitkus R, and Lowrey TK (1997) Genetics of adaptive radiation in Hawaiian and Cook Islands species of Tetramolopium (Asteraceae; Astereae). I. Nuclear RFLP marker diversity. Am J Bot 84(9): 1236-1246.
9. Rether B, Delmas G, and Laouedj A (1993) Isolation of polysaccharide-free DNA from plants. Plant Mol Biol Rep 11(4): 333-337.
10. Rogstad SH (1993) Surveying plant genomes for variable number of tandem repeat loci. Meth Enzymol 224: 278-294.
11. Scott KD and Playford J (1996) DNA extraction technique for PCR in rain forest plant species. Biotechniques 20(6): 974-978.