Multiplex PCR for the Detection of Genes Encoding Aminoglycoside Modifying Enzymes and Methicillin Resistance among Staphylococcus Species

Journal of Korean Medical Science

Volumn 18, Issue 5, 2003, Pages 631-636

  Choi, Su Mi ^c   Kim, Seung Han ^a   Kim, Hee Jung ^a   Lee, Dong Gun ^c   Choi, Jung Hyun ^c   Yoo, Jin Hong ^c   Kang, Jin Han ^b   Shin, Wan Shik ^b,c   Kang, Moon Won ^c  

^a Seoul National University   (South Korea)

^b The Catholic University of Korea   (South Korea)

^c The Catholic University of Korea   (South Korea)

Author keywords

Aminoglycoside; Aminoglycoside Modifying Enzyme; Antibiotics; Methicillin Resistance; Polymerase Chain Reaction; Staphylococcus aureus

Indexed keywords

AMIKACIN; AMINOGLYCOSIDE ANTIBIOTIC AGENT; AMINOGLYCOSIDE MODIFYING ENZYME; GENTAMICIN; ISOENZYME; KANAMYCIN; METICILLIN; OXACILLIN; PENICILLIN BINDING PROTEIN 2A; TOBRAMYCIN; UNCLASSIFIED DRUG;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; CENTRAL NERVOUS SYSTEM; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; DILUTION; GENE IDENTIFICATION; GENETIC CODE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; PENICILLIN RESISTANCE; POLYMERASE CHAIN REACTION; UNIVERSITY HOSPITAL;

EID: 0348225446     PISSN: 10118934     EISSN: None     Source Type: Journal    
DOI: 10.3346/jkms.2003.18.5.631     Document Type: Article

References (24)

1. Christensen GD, Bisno AL, Parisi JT, McLaughlin B, Hester MG, Luther RW. Nosocomial septicemia due to multiply antibiotic-resistant Staphylococcus epidermidis. Ann Intern Med 1982; 96: 1-10.
2. Haley RW, Hightower AW, Khabbaz RF, Thornsberry C, Martone WJ, Allen JR, Hughes JM. The emergence of methicillin-resistant Staphylococcus aureus infections in United States hospitals. Ann Intern Med 1982; 97: 297-308.
3. Saravolatz LD, Markowitz N, Arking L, Pohlod D, Fisher E. Methicillin-resistant Staphylococcus aureus. Epidemiologic observations during a community-acquired outbreak. Ann Intern Med 1982; 96: 11-6.
4. Kim JM, Park ES, Jeong JS, Kim KM, Kim JM, Oh HS, Yoon SW, Lee SI, Lee MS, Song JH, Kang MW, Choe KW, Park SC, Pai CH. Nosocomial infection surveillance committee of the Korean society for nosocomial infection control. 1996 National nosocomial infection surveillance in Korea. Korean J Nosocom Infect Control 1997; 2: 157-76.
5. Geha DJ, Uhl JR, Gustaferro CA, Persing DH. Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory. J Clin Microbiol 1994; 32: 1768-72.
6. Madiraju MVVS, Brunner DP, Wilkinson BJ. Effects of temperature, NaCl, and methicillin on penicillin-binding proteins, growth, peptidoglycan synthesis, and autolysis in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1987; 31: 1727-33.
7. Chambers HF. Coagulase-negative staphylococci resistant to β-lactam antibiotics in vivo produce penicillin-binding protein 2a. Antimicrob Agents Chemother 1987; 31: 1919-24.
8. Ubukata K, Nonoguchi R, Matsuhashi M, Konno M. Expression and inducibility in Staphlycoccus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein. J Bacteriol 1989; 171: 2882-85.
9. Matsuhashi M, Song F, Ishino M, Wachi M, Doi M, Inoue M, Ubukata K, Yamashita N, Konno M. Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to β-lactam antibiotics in Staphylococcus aureus. J Bacteriol 1986; 167: 975-80.
10. Busch-Sorensen C, Frimodt-Moller N, Miller GH, Espersen F. Aminoglycoside resistance among Danish blood culture isolates of coagulase-negative staphylococci. APMIS 1996; 104: 873-80.
11. Shaw KJ, Rather PN, Hare RS, Miller GH. Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol Rev 1993; 57: 138-63.
12. Murakami K, Minamide W, Wada K, Nakamura E, Teraoka H, Watanabe S. Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J Clin Microbiol 1991; 29: 2240-44.
13. Vanhoof R, Godard C, Content J, Nyssen HJ, Hannecart-Pokorni E. Detection by polymerase chain reaction of genes encoding aminoglycoside- modifying enzymes in methicillin-resistant Staphylococcus aureus isolates of epidemic phage types. J Med Microbiol 1994; 41: 282-90.
14. Vannuffel P, Gigi J, Ezzedine H, Vandercam B, Delmee M, Wauters G, Gala JL. Gala. Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. J Clin Microbiol 1995; 33: 2864-67.
15. Martineau F, Picard FJ, Lansac N, Ménard C, Roy PH, Ouellette M, Bergeron MG. Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob Agents Chemother 2000; 44: 231-8.
16. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility testing; twelfth informational supplement. Document M100-S12. National Committee for Clinical Laboratory Standards, Wayne, Pa. 2002.
17. Martineau F, Picard FJ, Grenier L, Roy PH, Ouellette M, the ESPRIT Trial, Bergeron MG. Multiplex PCR assays for the detection of clinically relevant antibiotic resistance genes in staphylococci isolated from patients infected after cardiac surgery. J Antimicrob Chemother 2000; 46: 527-33.
18. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility testing. Document M100-S10. National Committee for Clinical Laboratory Standards, Wayne, Pa. 2001.
19. Gradelski E, Valera L, Aleksunes L, Bonner D, Fung-Tomc J. Correlation between genotype and phenotypic categorization of Staphylococci based on methicillin susceptibility and resistance. J Clin Microbiol 2001; 39: 2961-3.
20. Sabath LD. Mechanisms of resistance to beta-lactam antibiotics in strains of Staphylococcus aureus. Am Intern Med 1982; 97: 339-44.
21. Wyke AW, Ward JB, Hayes MV, Curtis NA. A role in vivo for penicillin-binding protein-4 of Staphylococcus aureus. Eur J Biochem 1981; 119: 389-93.
22. Ida T, Okamoto R, Shimauchi C, Okubo T, Kuga A, Inoue M. Identification of aminoglycoside-modifying enzymes by susceptibility testing: Epidemiology of methicillin-resistant Staphylococcus aureus in Japan. J Clin Microbiol 2001; 39: 3115-21.
23. Schmitz FJ, Fluit AC, Gondolf M, Beyrau R, Lindenlauf E, Verhoef J, Heinz HP, Jones ME. The prevalence of aminoglycoside resistance and corresponding resistance genes in clinical isolates of staphylococci from 19 European hospitals. J Antimicrob Chemother 1999; 43: 253-9.
24. Kim HB, Kim T, Lee BB, Kim US, Park SW, Shin JW, Oh MD, Kim EC, Lee YS, Kim BS, Choe KW. Frequency of resistance to aminoglycoside antibiotics in Staphylococcus aureus isolates from tertiary hospitals. Korean J Infect Dis 2002; 34: 39-46.