Generation of the R2 Subunit of Ribonucleotide Reductase by Intein Chemistry: Insertion of 3-Nitrotyrosine at Residue 356 as a Probe of the Radical Initiation Process

Biochemistry

Volumn 42, Issue 49, 2003, Pages 14541-14552

  Yee, Cyril S ^a   Seyedsayamdost, Mohammad R ^a   Chang, Michelle C Y ^a   Nocera, Daniel G ^a   Stubbe, JoAnne ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CATALYSIS; CHITIN; CHROMATOGRAPHIC ANALYSIS; ELECTRONS; ESCHERICHIA COLI; ORGANIC ACIDS;

ELECTRON TRANSFER;

ENZYMES;

3 NITROTYROSINE; AMINO ACID; CHITIN; CYSTEINE; DEOXYNUCLEOSIDE DIPHOSPHATE DERIVATIVE; INTEIN; IRON; IRON DERIVATIVE; MESNA; NUCLEOSIDE DERIVATIVE; NUCLEOSIDE DIPHOSPHATE DERIVATIVE; NUCLEOTIDE; OXYGEN; PEPTIDE; RADICAL; RIBONUCLEOTIDE REDUCTASE; SERINE; THIOESTER; TYROSINE DERIVATIVE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ALLOSTERISM; AMINO ACID SUBSTITUTION; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BINDING SITE; CATALYST; ELECTRON TRANSPORT; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME METABOLISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ENZYME SUBUNIT; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE INSERTION; MOLECULAR PROBE; NONHUMAN; PEPTIDE SYNTHESIS; PKA; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTON COUPLED ELECTRON TRANSFER; REDUCTION; SITE DIRECTED MUTAGENESIS; TECHNIQUE; WILD TYPE;

AMINO ACID SEQUENCE; CHITIN; ELECTROCHEMISTRY; ESCHERICHIA COLI PROTEINS; ESTERS; FREE RADICALS; HYDROLYSIS; KINETICS; LIGANDS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE CHAIN INITIATION, TRANSLATIONAL; PEPTIDE FRAGMENTS; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; RIBONUCLEOTIDE REDUCTASES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TYROSINE;

ESCHERICHIA COLI; INSERTION SEQUENCES; NEGIBACTERIA;

EID: 0348111211     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0352365     Document Type: Article

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