A Light and Electron Microscopic Study of GAT-1 Positive Cells in the Monkey Brainstem and Spinal Cord

Journal of Brain Research

Volumn 39, Issue 4, 1999, Pages 551-557

  Ng, C H ^a   Ong, W Y ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Brainstem; GABA; Gaba transporter; GAT 1; Monkey; Neurons; Spinal cord; Synapses

Indexed keywords

4 AMINOBUTYRIC ACID CARRIER; CARRIER PROTEIN; GABA PERMEASE; MEMBRANE PROTEIN; ORGANIC ANION TRANSPORTER;

ANIMAL; ARTICLE; BRAIN STEM; CYTOLOGY; DENDRITE; ELECTRON MICROSCOPY; GLIA; IMMUNOHISTOCHEMISTRY; MACACA; MEDULLA OBLONGATA; MESENCEPHALON; METABOLISM; NERVE CELL; NERVE FIBER; PONS; SPINAL CORD; SYNAPSE; ULTRASTRUCTURE;

ANIMALS; AXONS; BRAIN STEM; CARRIER PROTEINS; DENDRITES; GABA PLASMA MEMBRANE TRANSPORT PROTEINS; IMMUNOHISTOCHEMISTRY; MACACA FASCICULARIS; MEDULLA OBLONGATA; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MESENCEPHALON; MICROSCOPY, ELECTRON; NEUROGLIA; NEURONS; ORGANIC ANION TRANSPORTERS; PONS; SPINAL CORD; SYNAPSES;

EID: 0347804736     PISSN: 09448160     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. BHART, P. M., JOHNSTON, G. A. R. and UHR, M. L.: Competitive inhibition of GABA uptake in rat brain slices by some GABA analogues of restricted conformation. J. Neurochem. 19, 1855-1861 (1972).
2. BORDEN, L. A., SMITH, K. E., HARTIG, P. R., BRANCHEK, T. A. and WEINSHANK, R. L.: Molecular heterogeneity of the aminobutyric acid (GABA) transport system. J. Biol. Chem. 267, 21098-21104 (1992).
3. CARPENTER, M. B.: Core text of neuroanatomy. Williams and Wilkins, Baltimore (1991).
4. DURKIN, M. M., SMITH, K. E., BORDEN, L. A., WEINSHANK, R. L., BRANCHEK, T. A. and GUSTAFEN, E. L.: Localization of messenger RNAs encoding three GABA transporters in rat brain: an in situ hybridization study. Mol. Brain Res. 33, 7-21 (1995).
5. GUASTELLA, J., NELSON, N., NELSON, H., CZYZYK, L., KEYMAN, S., MIEDEL, M. C., DAVIDSON, N., LESTER, H. A. and KANNER, B. I.: Cloning and expression of a rat brain GABA transporter. Science 249, 1303-1306 (1990).
6. HUNT, S. P., KELLY, J. S., EMSON, P. C., KIMMEL, J. R. and WU, J. Y.: An immunohistochemical study of neuronal populations containing neuropeptides or gamma-amminobutyrate within the superficial layer of the rat dorsal horn. Neuroscience 6, 1883-1898 (1981).
7. IKEGAKI, N., SAITO, N., HASHIMA, M. and TANAKA, C.: Production of specific antibodies against GABA transporter subtypes (GAT-1, GAT-2, GAT-3) and their application to immunocytochemistry. Mol. Brain Res. 26, 47-54 (1994).
8. ILIAKIS, B., ANDERSON, N. L., IRISH, P. S., HENRY, M. A. and WESTRUM, L. E.: Electron microscopy of immunoreactivity patterns for glutamate and gamma-aminobutyric acid in synaptic glomeruli of the feline spinal trigeminal nucleus (subnucleus caudalis). J. Comp. Neurol. 366, 465-477 (1996).
9. IVERSEN, L. L. and KELLY, J. S.: Uptake and metabolism of gamma-aminobutyric acid by neurons and glial cells. Biochem. Pharmacol. 24, 933-938 (1975).
10. KEYMAN, S., SUH, Y.-J., KANNER, B. I. and RUDNICK, G.: Expression of a cloned gamma-aminobutyric acid transporter in mammalian cells. Biochemistry 31, 1974-1979 (1992).
11. MINELLI, A., BRECHA, N. C., KARSCHIN, C., DEBIASI, S. and CONTI, F.: GAT-1, a high affinity GABA plasma membrane transporter, is localised to neurons and astroglia in the cerebral cortex. J. Neurosci. 15, 7734-7746 (1995).
12. ONG, W. Y., YEO, T. T., BALCAR, V. J. and GAREY, L. J.: A light and electron microscopic study of GAT-1 positive cells in the cerebral cortex of man and monkey. J. Neurocytol. 27, 719-730 (1998).
13. OTTERSEN, O. P. and STORM-MATHISEN, J.: Neurons containing or accumulating transmitter amino acids. In: Handbook of Chemical Neuroanatomy, 3. Classical Transmitters and Transmitter Receptors in the CNS, Part II. pp. 141-246 (1984).
14. SWAN, M., NAJLERAHIM, A., WATSON, R. E. B. and BENNET, J. P.: Distribution of mRNA for the GABA transporter GAT-1 in the rat brain: evidence that GABA uptake is not limited to presynaptic neurons. J. Anat. 185, 315-323 (1994).
15. TODD, A. J. and MCKENZIE, J.: GABA-immunoreactive neurons in the dorsal horn of the rat spinal cord. Neuroscience 31, 799-806 (1989).
16. VALTSCHANOFF, J. G., PHEND, K. D., BERNARDI, P. S., WEINBURG, R. J. and RUSTIONI, A.: Aminoacid immunocytochemistry of primary afferent terminals in rat dorsal horn. J. Comp. Neurol. 346, 237-252 (1994).
17. YASUMI, M., SATO, K., SHIMADA, S., NISHIMURA, M. and TOHYAMA, M.: Regional distribution of GABA transporter 1 (GAT1) mRNA in the rat brain: Comparison with glutamic acid decarboxylase67 (GAD67) mRNA localization. Mol. Brain Res. 44, 205-218 (1997).