Degradation of connexins from the plasma membrane is regulated by inhibitors of protein synthesis

Cell Communication and Adhesion

Volumn 10, Issue 4-6, 2003, Pages 329-333

  Vanslyke, Judy K ^a   Musil, Linda S ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Connexins; Cycloheximide; Degradation; Gap junctions; Plasma membrane

Indexed keywords

BIOTIN; CONNEXIN 32; CONNEXIN 43; CYCLOHEXIMIDE; DYE; GAP JUNCTION PROTEIN; MEMBRANE PROTEIN; PROTEIN SYNTHESIS INHIBITOR;

ANIMAL CELL; ARTICLE; BIOTINYLATION; CELL COMMUNICATION; CELL MEMBRANE; CELL SURFACE; CONTROLLED STUDY; DOWN REGULATION; GAP JUNCTION; IMMUNOFLUORESCENCE MICROSCOPY; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN TRANSPORT; REGULATORY MECHANISM; STAINING;

ANIMALIA;

EID: 0347756804     PISSN: 15419061     EISSN: None     Source Type: Journal    
DOI: 10.1080/cac.10.4-6.329.333     Document Type: Article

References (19)

1. Fallon RF, Goodenough DA (1981). Five-hour half-life of mouse liver gap-junction protein. J Cell Biol 90: 521-526.
2. Fishman GI, Gao Y, Hertzberg EL, Spray DC (1995). Reversible intercellular coupling by regulated expression of a gap junction channel gene. Cell Adhes Commun 3: 353-365.
3. Gaietta G, Deerinck TJ, Adams SR, Bouwer J, Tour O, Laird DW, Sosinsky GE, Tsien RY, Ellisman MH (2002). Multicolor and electron microscopic imaging of connexin trafficking. Science 296: 503-507.
4. Hare J, Taylor K (1991). Mechanisms of plasma membrane degradation: Recycling proteins are degraded more rapidly than those confined to the cell surface. Proc Nat Acad Sci 88: 5902-5906.
5. Musil LS, Le AC, VanSlyke JK, Roberts LM (2000). Regulation of connexin degradation as a mechanism to increase gap junction assembly and function. J Biol Chem 275: 25207-25215.
6. VanSlyke JK, Musil LS (2002). Dislocation and degradation from the ER are regulated by cytosolic stress. J Cell Biol 157: 381-394.
7. VanSlyke JK, Deschenes SM, Musil LS (2000). Intracellular transport, assembly, and degradation of wild-type and disease-linked mutant gap junction proteins. Mol Biol Cell 11: 1933-1946.
8. Jordan K, Chodock R, Hand AR, Laird DW (2001). The origin of annular junctions: A mechanism of gap junction internalization. J Cell Sci 114: 763-773.
9. Musil LS, Goodenough DA (1991). Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques. J Cell Biol 115: 1357-1374.
10. Nagy JI, Li WE, Roy C, Doble BW, Gilchrist JS, Kardami E, Hertzberg EL (1997). Selective monoclonal antibody recognition and cellular localization of an unphosphorylated form of connexin43. Exp Cell Res 236: 127-136.
11. Lippincott-Schwartz J, Yuan L, Tipper C, Amherdt M, Orci L, Klausner RD (1991). Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell 67: 601-616.
12. el-Fouly MH, Trosko JE, Chang CC (1987). Scrape-loading and dye transfer. A rapid and simple technique to study gap junctional intercellular communication. Exp Cell Res 168: 422-430.
13. Musil LS, Cunningham BA, Edelman GM, Goodenough DA (1990). Differential phosphorylation of the gap junction protein connexin43 in junctional communication-competent and -deficient cell lines. J Cell Biol 111: 2077-2088.
14. Castro C, Gomez-Hernandez JM, Silander K, Barrio LC (1999). Altered formation of hemichannels and gap junction channels caused by C-terminal connexin-32 mutations. J Neurosci 19: 3752-3760.
15. Musil LS, Beyer EC, Goodenough DA (1990). Expression of the gap junction protein connexin43 in embryonic chick lens: Molecular cloning, ultrastructural localization, and post-translational phosphorylation. J Membr Biol 116: 163-175.
16. Jiang JX, Goodenough DA (1998). Phosphorylation of lens-fiber connexins in lens organ cultures. Eur J Biochem 255: 37-44.
17. Berthoud VM, Bassnett S, Beyer EC (1999). Cultured chicken embryo lens cells resemble differentiating fiber cells in vivo and contain two kinetic pools of connexin56. Exp Eye Res 68: 475-484.
18. Watanabe M, Kobayashi H, Rutishauser U, Katar M, Alcala J, Maisel H (1989). NCAM in the differentiation of embryonic lens tissue. Dev Biol 135: 414-423.
19. Katar M, Alcala J, Maisel H (1993). NCAM of the mammalian lens. Curr Eye Res 12: 191-196.