Rapid Selection of Nonhotspot Mutants among hisD+ Revertants of Salmonella typhimurium TA98 in Ames Test by Peptide Nucleic Acid (PNA)-Mediated PCR Clamping

Journal of Bioscience and Bioengineering

Volumn 96, Issue 6, 2003, Pages 588-590

  Takiya, Toshiyuki ^a,b   Horie, Yoshiaki ^a   Futo, Satoshi ^b   Matsumoto, Yutaka ^c   Kawai, Keiichi ^a   Suzuki, Tohru ^a  

^a GIFU UNIVERSITY   (Japan)

^b Kanagawa 243 0041   (Japan)

^c Hokkaido Res Inst Environ Sci   (Japan)

Author keywords

Ames test; DNA sequencing; Hotspot mutation; Mutagen; PCR clamping; Peptide nucleic acid; Pollution

Indexed keywords

GENETIC ENGINEERING; MUTAGENESIS; NUCLEIC ACID SEQUENCES; NUCLEIC ACIDS;

AMES TEST;

BACTERIA;

HISTIDINOL DEHYDROGENASE; PEPTIDE NUCLEIC ACID;

AMES TEST; ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; CONTROLLED STUDY; COST; GENE AMPLIFICATION; METHODOLOGY; MUTATIONAL ANALYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; REVERTANT; SALMONELLA TYPHIMURIUM;

BACTERIA (MICROORGANISMS); SALMONELLA TYPHIMURIUM;

EID: 0347755632     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.96.588     Document Type: Article

References (8)

1. Maron, D. M. and Ames, B. N.: Revised methods for Salmonella mutagenicity test. Mutat. Res., 113, 173-215 (1983).
2. Levine, J. G., Schaaper, R. M., and DeMarini, D. M.: Complex frameshift mutations mediated by plasmid pKM101: mutation mechanisms deduced from 4-aminobiphenyl-induced mutation spectra in Salmonella. Genetics, 136, 731-746 (1994).
3. Abu-Shakra, A., McQueen, E. T., and Cunningham, M. L.: Rapid analysis of base-pair substitutions induced by mutagenic drugs through their oxygen radical or epoxide derivatives. Mutat. Res., 470, 11-18 (2000).
4. Kupchella, E. and Cebula, T. A.: Analysis of Salmonella typhimwium hisD 3052 revertants: the use of oligodeoxyribonucleotide colony hybridization, PCR, and direct sequencing in mutational analysis. Environ. Mol. Mutagen., 18, 224-230 (1991).
5. DeMarini, D. M.: Mutation spectra of complex mixtures. Mutat. Res., 411, 11-18 (1998).
6. Nielsen, P. E., Egholm, M., Berg, R. H., and Buchardt, O.: Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science, 254, 1497-1500 (1991).
7. Orum, H., Nielsen, P. E., Egholm, M., Berg, R. H., Buchardt, O., and Stanley, C.: Single base pair mutation analysis by PNA directed PCR clamping. Nucleic Acids Res., 21, 5332-5336 (1993).
8. Ausubel, F. M.: Current protocols in molecular biology, p. 2.4.1-2.4.2. John Wiley & Sons, New York (1987).