Proteolytic Sensitivity of a Recombinant Phospholipase D from Cabbage: Identification of Loop Regions and Conformational Changes

Journal of Protein Chemistry

Volumn 22, Issue 6, 2003, Pages 499-508

  Younus, H ^a,b   Schops R ^b   Lerchner, A ^b   Rucknagel K P ^c   Schierhorn, A ^b   Saleemuddin, M ^a   Ulbrich Hofmann, R ^b  

^a ALIGARH MUSLIM UNIVERSITY   (India)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^c M P Res U Enzymol Protein F   (Germany)

Author keywords

Calcium; Conformation; Limited proteolysis; Mutant; Phospholipase D

Indexed keywords

1,3 DIPALMITOYLGLYCERO 2 PHOSPHOCHOLINE; ASPARAGINE; CALCIUM ION; DIPALMITOYLPHOSPHATIDYLCHOLINE; GLYCINE; ISOLEUCINE; LEUCINE; PHOSPHOLIPASE D; RECOMBINANT ENZYME; SERINE; SODIUM ION; THREONINE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CABBAGE; CATALYSIS; CONFORMATIONAL TRANSITION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BRASSICA; CALCIUM; CHYMOTRYPSIN; HEAT; LYSINE; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PHOSPHOLIPASE D; PROTEIN CONFORMATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; SERINE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; THERMOLYSIN; TRYPSIN;

BRASSICA OLERACEA VAR. CAPITATA;

EID: 0347479262     PISSN: 02778033     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:JOPC.0000005498.13074.72     Document Type: Article

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