Signal amplification by combining two advanced immunohistochemical techniques

European Journal of Histochemistry

Volumn 47, Issue 4, 2003, Pages 379-384

  Russo, D ^a   Ambrosino, A ^a   Vittoria, A ^a   Cecio, A ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

EnVision + system; EVS MICA method; Immunohistochemistry; MICA method; Signal amplification

Indexed keywords

AVIDIN; BIOTIN; CHROMOGRANIN A; NEURONAL NITRIC OXIDE SYNTHASE; VASOACTIVE INTESTINAL POLYPEPTIDE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DEVICE; ENDOCRINE CELL; IMMUNOHISTOCHEMISTRY; INTESTINE; NERVE CELL; NERVE FIBER; NONHUMAN; PERIKARYON; SENSITIVITY AND SPECIFICITY; SHEEP; SWINE;

ANIMALIA; MICA; OVIS ARIES; SUS SCROFA;

EID: 0347419942     PISSN: 1121760X     EISSN: None     Source Type: Journal    
DOI: 10.4081/850     Document Type: Article

References (22)

1. Adams JC. Heavy metal intensification of DAB-based HRP reaction product. J Histochem Cytochem 1981; 29:775.
2. Adams JC. Biotin amplification of biotin and horseradish peroxidase signal in histochemical stains. J Histochem Cytochem 1992; 40:1457-63.
3. Bisgaard K, Pulzek K. Use of polymer conjugates in immunohisto-chemistry: a comparative study of a traditional staining method to a staining method utilizing polymer conjugates. Pathol Int 1996; 46 (suppl. 1):577.
4. Cecio A, Vittoria A, Budetta G, Corona A. Staining of neuroendocrine cells by Linder's argyrophil method. J Anat 1988; 157:197-201.
5. Davidoff M, Schulze W. Combination of the peroxidase anti-peroxidase (PAP)- and avidin-biotin-peroxidase complex (ABC)-techniques: an amplification alternative in immunocytochemical staining. Histochem 1990; 93:531-6.
6. Fujita T. Paraneuron, its current implications. Biomed Res 1980; 1 (suppl.):3-9.
7. Gallyas F, Görcs T, Merchenthaler I. High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry. J Histochem Cytochem 1982; 30:183-84.
8. Heras A, Roach CM, Key ME. Enhanced polymer detection system for immunohistochemistry. Mod Pathol 1995; 8:165A.
9. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 1981; 29:577-80.
10. Hsu SM, Soban E. Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. J Histochem Cytochem 1982; 30:1079-82.
11. Itoh K, Konishi A, Nomura S, Mizuno N, Nakamura Y, Sugimoto T. Application of coupled oxidation reaction to electron microscopic demonstration of horseradish peroxidase: cobalt-glucose oxidase method. Brain Res 1979; 175:341-46,
12. Linder JE. A simple and reliable method for the silver impregnation of nerves in paraffin sections of soft and mineralized tissues. J Anat 1978; 127:543-51.
13. Mangham DC, Isaacson PG. A novel immunohistochemical detection system using mirror image complementary antibodies (MICA). Histopathol 1999; 35:129-33.
14. Mangham DC, Bradwell AR, Isaacson PG. MICA-a highly sensitive and avidin-free immunohistochemical detection system. Adv Anat Pathol 2000; 7:360-4.
15. Merchenthaler I, Stankovics J, Gallyas F. A highly sensitive one-step method for silver intensification of the nickel-diaminobenzidine end-product of peroxidase reaction. J Histochem Cytochem 1989; 37:1563-5.
16. Pearse AGE. The diffuse neuroendocrine system and the APUD concept: related "endocrine" peptides in brain, intestine, pituitary, placenta and anuran cutaneous glands. Med Biol 1977; 55:115-25.
17. Sabattini E, Bisgaard K, Ascani S, Poggi S, Piccioli M, Ceccarelli C, et al, The EnVisionTM+ System: a new immunohistochemical method for diagnostic and research. Critical comparison with the APAAP, ChemMate, CSA, LABC, and SABC techniques. J Clin Pathol 1998; 51:506-11.
18. Shu S, Ju G, Fan L. The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system. Neurosci Lett 1988; 85:169-71.
19. Straus W. Irnidazole increase the sensitivity of cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH. J Histochem Cytochem 1982; 30:491-3.
20. Swanson PE, Hagen KA, Wick MR. Avidin-biotin-peroxidase-antiperoxidase (ABPAP) complex. An immunocytochemical method with enhanced sensitivity. Am J Clin Pathol 1987; 88:162-76.
21. Vacca LL, Rosario S, Zimmerman EA, Tomachefsky P, Po-Ying NG, Hsu KC. Application of immunoperoxidase techniques to localize horseradish peroxidase-tracer in the central nervous system. J Histochem Cytochem 1975; 23:208-15.
22. Vyberg M, Nielsen S. Dextran polymer conjugate two-step visualization system for immunohistochemistry. A comparison of Envision+ with two three step avidin-biotin techniques. Appl Immunohistochem 1998; 6:3-10.