Immunocompetent Infants as a Human Reservoir for Pneumocystis jirovecii: Rapid Screening by Non-Invasive Sampling and Real-Time PCR at the Mitochondrial Large Subunit rRNA Gene

Journal of Eukaryotic Microbiology

Volumn 50, Issue SUPPL., 2003, Pages 668-669

  Totet, Anne ^a   Meliani, Leila ^b   Lacube, Philippe ^b   Pautard, Jean Claude ^a   Raccurt, Christian ^a   Roux, Patricia ^b   Nevez, Gilles ^a  

^a AMIENS UNIVERSITY HOSPITAL   (France)

^b HÔPITAL SAINT ANTOINE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA; FUNGAL RNA; MITOCHONDRIAL DNA; PRIMER DNA; RIBOSOME RNA;

POLYMERASE CHAIN REACTION;

BRONCHIOLITIS; CONFERENCE PAPER; DISEASE CARRIER; GENE AMPLIFICATION; GENETICS; HUMAN; IMMUNOCOMPETENCE; IMMUNOLOGY; INFANT; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; NUCLEOTIDE SEQUENCE; PNEUMOCYSTIS JIROVECI; PNEUMOCYSTIS PNEUMONIA; POLYMERASE CHAIN REACTION; REPORTER GENE;

BASE SEQUENCE; BRONCHIOLITIS; DISEASE RESERVOIRS; DNA PRIMERS; DNA, FUNGAL; DNA, MITOCHONDRIAL; GENE AMPLIFICATION; GENES, REPORTER; HUMANS; IMMUNOCOMPETENCE; INFANT; PNEUMOCYSTIS JIROVECI; PNEUMONIA, PNEUMOCYSTIS; POLYMERASE CHAIN REACTION; RNA, FUNGAL; RNA, RIBOSOMAL;

PNEUMOCYSTIS; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS JIROVECII;

EID: 0347388403     PISSN: 10665234     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1550-7408.2003.tb00678.x     Document Type: Conference Paper

References (5)

1. Larsen, H.H., Masur, H., Kovacs, J.A., Gill, V.J., Silcott, V.A., Kogulan, P., Maenza J., Smith, M., Lucey, D.R. & Fisher, S.H. 2002. Development and evaluation of a quantitative, touch-down, real-time PCR assay for diagnosing Pneumocystis carinii pneumonia. J. Clin. Microbiol., 40:490-494.
2. Nevez, G., Totet, A. & Raccurt, C. 2001. Pneumocystis carinii detection using nested-PCR in nasopharyngeal aspirates of immunocompetent infants with bronchiolitis. J. Eukaryot. Microbiol., 48:122-123S.
3. Vargas, S.L., Hughes, W.T., Santolaya, M.E., Ulloa, V., Ponce, C.A., Cabrera, C.E., Cumsille, F. & Gigliotti, F. 2001. Search for primary infection by Pneumocystis carinii in a cohort of normal healthy infants. Clin. Infec. Dis., 32:855-861.
4. Wakefield, A.E., Pixley, F.J., Banerji, S., Sinclair, K., Miller, R.F., Moxon, E.R. & Hopkin, J.M. 1990. Detection of Pneumocystis carinii with DNA amplification. Lancet, 336:451-453.
5. Wakefield, A.E. 1996. DNA sequences identical to Pneumocystis carinii f.sp. carinii and Pneumocystis carinii f.sp. hominis in samples of air spora. J. Clin. Microbiol., 34:1754-1759.