Attempt to map the receptor binding sites of human follicle-stimulating hormone using disulfide peptides of its β-subunit indicates major involvement of the regions around disulfide bonds Cys28-Cys 82 and Cys32-Cys84 in receptor binding of the hormone

Journal of Peptide Research

Volumn 62, Issue 6, 2003, Pages 269-279

  Jetly, N M ^a   Iyer, K S ^a   Hosur, M V ^b   Mahale, S D ^a  

^a NATIONAL INSTITUTE FOR RESEARCH IN REPRODUCTIVE HEALTH   (India)

^b BHABHA ATOMIC RESEARCH CENTRE   (India)

Author keywords

Directed synthesis of disulfide peptides; Disulfide peptides of the subunit; Follicle stimulating hormone; Radioreceptor assay; Receptor binding regions

Indexed keywords

CYSTEINE; DISULFIDE; FOLLITROPIN; FOLLITROPIN RECEPTOR; IODINE 125; PEPTIDE DERIVATIVE; PROTEIN SUBUNIT;

ADENOHYPOPHYSIS; ALPHA CHAIN; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BETA CHAIN; BINDING SITE; CHEMICAL BOND; COMPETITIVE INHIBITION; CONTROLLED STUDY; CROSS LINKING; DISULFIDE BOND; FOLLITROPIN RELEASE; HORMONE ACTION; HORMONE RECEPTOR INTERACTION; HORMONE STRUCTURE; HUMAN; ISOTOPE LABELING; MOLECULAR STABILITY; NONHUMAN; OVARY FOLLICLE DEVELOPMENT; PEPTIDE ANALYSIS; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; RAT; RECEPTOR OCCUPANCY; SPERMATOGENESIS;

AMINO ACID SEQUENCE; BINDING SITES; CYSTEINE; DISULFIDES; FOLLICLE STIMULATING HORMONE, HUMAN; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; PROTEIN SUBUNITS; RECEPTORS, FSH;

ANIMALIA;

EID: 0347384216     PISSN: 1397002X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1399-3011.2003.00096.x     Document Type: Article

References (37)

1. Foulkes, N.S., Schlotter, F., Pevet, P. & Sassone-Corsi, P. (1993) Pituitary hormone FSH directs the CREM functional switch during spermatogenesis. Nature 362, 264-267.
2. Richards, J.S. (1980) Maturation of ovarian follicles actions and interactions of pituitary and ovarian hormones on follicular cell differentiation. Physiol Rev. 60, 51-89.
3. Parsons, T.F., Strickland, T.W. & Pierce, J.G. (1985) Disassembly and assembly of glycoprotein hormones. Methods Enzymol. 109, 736-749.
4. Pierce, J.G. & Parsons, T.F. (1981) Glycoprotein hormones: structure and function. Annu. Rev. Biochem. 50, 465-495.
5. Fujiki, Y., Rathnam, P. & Saxena, B.B. (1980) Studies on the disulfide bonds in human follicle-stimulating hormone. Biochim. Biophy. Acta 624, 428-435.
6. Santa-Coloma, T.A., Dattatreyamurty, B. & Reichert, L.E. Jr (1990) A synthetic peptide corresponding to human FSH beta-subunit 33-53 binds to FSH receptor, stimulates basal estradiol biosynthesis and is a partial antagonist of FSH. Biochemistry 29, 1194-1200.
7. Santa-Coloma, T.A. & Reichert, L.E. Jr (1990) Identification of a follicle-stimulating hormone receptor-binding region in hFSHβ-(81-95) using synthetic peptides. J. Biol. Chem. 265, 5037-5042.
8. Reichert, L.E. Jr (1994) The functional relationship between FSH and its receptor as studied by synthetic peptide strategies. Mol. Cell. Endocrinol. 100, 21-27.
9. Lal, D., Mahale, S.D. & Iyer, K.S. (1996) Studies on the delineation of the hormone-specific antigenic determinants of human follicle-stimulating hormone. Peptide Res. 9, 195-201.
10. Dias, J.A. (1996) Human follitropin heterodimerization and receptor binding structural motifs: identification and analysis by a combination of synthetic peptide and mutagenesis approaches. Mol. Cell. Endocrinol. 125, 45-54.
11. Dias, J.A. (2002) Human follitropin: structure-activity relationships. Indian J. Expt. Biol. 40, 385-392.
12. Guidice, L.C. & Pierce, J.G. (1978) Studies on the reduction and reoxidation of the disulfide bonds of the α and β subunits of human chorionic gonadotropin. Biochim. Biophy. Acta 533, 140-146.
13. Suganuma, N., Matzuk, M.M. & Boime, I. (1989) Elimination of disulfide bonds affects assembly and secretion of the human chorionic gonadotropin β-subunit. J. Biol. Chem. 264, 19302-19307.
14. Bahl, O.P., Pandian, M.R. & Ghai, R.D. (1976) Immunological properties of the β-subunit of human chorionic gonadotropin. Biochem. Biophys. Res. Commun. 70, 525-532.
15. Ghai, R.D., Mise, T., Pandian, M.R. & Bahl, O.P. (1980) Immunological properties of the β-subunit of human chorionic gonadotropin. I. Effects of chemical and enzymatic modification. Endocrinology 107, 1556-1563.
16. 72 in receptor binding of the hormone. J. Peptide Res. 58, 17-26.
17. Lapthorn, A.J., Harris, D.C., Littlejohn, A., Lustbader, J.W., Canfield, R.E., Machin, K.J., Morgan, F.J. & Isaacs, N.W. (1994) Crystal structure of human chorionic gonadotropin. Nature 369, 455-461.
18. 90 of the β-subunit of human chorionic gonadotropin are crucial for heterodimer formation with the α-subunit: experimental evidence for the conclusions from the crystal structure of hCG. Biochim. Biophy. Acta 1645, 49-55.
19. Fox, K.M., Dias, J.A. & Van Roey, P. (2001) Three dimensional structure of follicle stimulating hormone. Mol. Endocrinol. 15, 378-389.
20. Ellman, G.L. (1959) Tissue sulphydryl groups. Arch. Biochem. Biophys. 82, 70-77.
21. Akaji, K., Fujino, K., Tatsumi, T. & Kiso, Y. (1993) Total synthesis of human insulin by regioselective disulfide formation using the silyl chloride-sulfoxide method. J. Am. Chem. Soc. 115, 11384-11392.
22. Kudryavtseva, E.V., Sidorova, M.V., Orchinnikov, M.V., Bespalova, Z.D. & Bushuev, V.N. (1997) Comparative evaluation of different methods for disulfide bond formation in synthesis of the HIV-2 antigenic determinant. Peptide Res. 49, 52-58.
23. Reichert, L.E. Jr & Issac, H.A. (1977) Studies on a low molecular weight testicular factor which inhibits binding of FSH to receptor. Biol. Reprod. 17, 614-621.
24. Fraker, P.J. & Speck, J.C. Jr (1978) Protein and cell membrane iodinations with a sparingly soluble chloramide 1,3,4,6-tetrachloro-3α, 6α-diphenyl glycoluril. Biochem. Biophy. Res. Commun. 80, 849-857.
25. Selvaraj, N. & Moudgal, N.R. (1993) Development of an LH receptor assay capable of measuring serum LH/CG in a wide variety of species. J. Reprod. Fertility 98, 611-616.
26. Roth, K.E. & Dias, J.A. (1995) Scanning-alanine mutagenesis of long loop residues 33-53 in follicle stimulating hormone β-subunit. Mol. Cell. Endocrinol. 109, 143-149.
27. Lindau-Shephard, B., Roth, K.E. & Dias, J.A. (1994) Identification of amino acids in the C-terminal region of human follicle-stimulating hormone (FSH) β-subunit involved in binding to human FSH receptor. Endocrinology 135, 1235-1240.
28. Campbell, R.K., Dean-Emig, D.M. & Moyle, W.R. (1991) Conversion of human chorionic gonadotropin into a follitropin by protein engineering. Proc. Nat. Acad. Sci. USA 88, 760-764.
29. Braun, T., Schofield, P.R. & Sprengel, R. (1991) Amino-terminal leucine-rich repeats in gonadotropin receptors determine hormone selectivity. EMBO J. 10, 1885-1890.
30. Dattatreyamurthy, B. & Reichert, L.E. Jr (1993) Functional properties of polyclonal antibodies raised against the N-terminus region (residues 9-30) of the follicle-stimulating hormone (FSH) receptor: significance of this receptor region in FSH recognition and signal transduction. Endocrinology 133, 1593-1601.
31. Dattatreyamurty, B. & Reichert, L.E. Jr (1992) A synthetic peptide corresponding to amino acids 9-30 of the extracellular domain of the follitropin (FSH) receptor specifically binds FSH. Mol. Cell. Endocrinol. 87, 9-17.
32. Hiro'oka, T., Maassen, D., Berger, P. & Boime, I. (2000) Disulfide bond mutations in follicle-stimulating hormone result in uncoupling of biological activity from intracellular behaviour. Endocrinology 141, 4751-4756.
33. Ward, D.N. & Moore, W.T. Jr (1979) Comparative studies of mammalian glycoprotein hormones. In: Animals Models for Research on Contraception and Fertility (Alexander, N.J., ed.). Harper and Row, Baltimore, MD, USA, pp. 151-163.
34. Keutmann, H.T., Mason, K.A., Kitzman, K. & Ryan, R.J. (1989) Role of the β(93-100) determinant loop sequence in receptor binding and biological activity of human luteinizing hormone and chorionic gonadotropin. Mol. Endocrinol. 3, 526-531.
35. Roth, K.E., Liu, C., Shepard, B.A., Shaffer, J.B. & Dias, J.A. (1993) The flanking amino acids of the human follitropin β-subunit 33-53 region are involved in assembly of the follitropin heterodimer. Endocrinology 131, 2571-2577.
36. Roth, K.E. & Dias, J.A. (1996) Follitropin conformational stability mediated by loop 2β effects follitropin-receptor interaction. Biochemistry 35, 7928-7935.
37. Jagtap, D.D., Mahale, S.D., Mishra, A.K., Nandedkar, T.D. & Iyer, K.S. (2002) A detailed study of the L2β long-loop region of human chorionic gonadotropin suggests it to be spatially close to, but not part of, the receptor-binding site. J. Endocrinol. 172, 311-320.