Identification of a NF-κB site in the negative regulatory element (ε-NRAII) of human ε-globin gene and its binding protein NF-κB p50 in the nuclei of K562 cells

Cell Research

Volumn 12, Issue 1, 2002, Pages 79-82

  Hou, Chun Hui ^a   Huang, Jian ^a   Qian, Ruo Lan ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

Human globin gene; Negative regulatory element; NF B p50

Indexed keywords

DNA; GLOBIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; NUCLEAR PROTEIN; TRANSCRIPTION FACTOR REL;

ARTICLE; BINDING SITE; CELL NUCLEUS; CELL STRAIN K 562; CHEMISTRY; CONSENSUS SEQUENCE; DNA SEQUENCE; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; HUMAN; METABOLISM; MOLECULAR WEIGHT; MUTATION; NUCLEOTIDE SEQUENCE; REGULATORY SEQUENCE;

BASE SEQUENCE; BINDING SITES; CELL NUCLEUS; CONSENSUS SEQUENCE; DNA; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GLOBINS; HUMANS; K562 CELLS; MOLECULAR WEIGHT; MUTATION; NF-KAPPA B; NUCLEAR PROTEINS; PROTO-ONCOGENE PROTEINS C-REL; REGULATORY SEQUENCES, NUCLEIC ACID; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION, GENETIC;

EID: 0347367388     PISSN: 10010602     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cr.7290113     Document Type: Article

References (23)

1. Karlsson S, Nienhuis AW. Developmental regulation of human globin gene. Ann Rev Biochem 1985; 54:1071-108.
2. Raich N, Enver T, Nakamoto B et al. Autonomous development control of human embryonic globin gene switching in transgenic mice. Science 1990; 250:1147-9.
3. Shih DM, Wall RJ, Shapiro SG. Developmentally regulated and erythroid-specific expression of the human embryonicε-globin gene in transgenic mice. Nucleic Acids Res 1990; 18:5465-72.
4. Bauerle PA, T Henkel. Function and activation of NF-κB in the immune system. Annu Rev Immunol 1994; 12:141-79.
5. Jr Baldwin AS. The NF-κB and I-κB proteins: new discoveries and insights. Annu Rev Immunol 1996; 14:649-83.
6. Ghosh S, MJ May, EB Kopp. NF-κB and rel proteins: evolutionary conserved mediators of immune responses. Annu Rev Immunol 1998; 16:225-60.
7. Frankenberger M, Pforte A, Sternsdorf T, Passlick B, Baeuerle PA, Ziegler-Heitbrock HW. Constitutive nuclear NF-kappa B in cells of the monocyte lineage. Biochem J 1994; 15; 304 (Pt 1):87-94.
8. Li J, Noguchi CT, Miller W, Hardson R, Schechter AN. Multiple regulatory elements in the 5'-flanking sequence of the human epsilon-globin gene. J Biol Chem 1998; 273: 10202-9.
9. Heinemeyer T et al. Expanding the TRANSFAC database towards an expert system of regulatory molecular mechanisms. Nucleic Acids Res 1999; 7:318-22.
10. Yan ZJ, Jiang C, Qian RL. Trans-acting factors from the human fetal liver binding to the human epsilon-globin gene silencer. Cell Res 1997; 7(2):151-9
11. Dignam JD, Lebovitz RM, Roeder RG. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 1983; 11(5):1475-89.
12. Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72(1):248-54.
13. Zabel U, Schreck R, Baeuerle PA. DNA binding of purified transcription factor NF-kappaB. J Biol Chem 1991; 266:252-60.
14. Strauss F, Varshavsky A. A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosomes. Cell 1984; 37:889-901.
15. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning A laboratory manual, 2nd ed, 1989; 888-98.
16. Perkins ND. Achieving transcriptional specificity with NF-kappa B. Int J Biochem Cell Biol 1997; 29(12):1433-48.
17. Lavrovsky Y, Schwartzman ML, Levere RD, Kappas A, Abraham NG. Identification of binding sites for transcription factors NF-κB and AP-2 in the promoter region of the human heme oxygenase 1 gene. Proc Natl Acad Sci USA 1994; 21; 91(13):5987-91.
18. Sater RA. Basal expression of the human macrophage colony-stimulating factor (M-CSF) gene in K562 cells. Leuk Res 1994; 18(2):133-43.
19. Li Q, Blau CA, Clegg CH et al. Multiple epsilon-promoter elements participate in the developmental control of epsilon-globin genes in transgenic mice. J Biol Chem 1998; 273:17361-67.
20. Raich N, Papayannopoulou T, Stamatoyannopoulos G et al. Demonstration of a human epsilon-globin gene silencer with studies in transgenic mice. Blood 1992; 79:861-4.
21. Raich N, Clegg CH, Grofti J et al. GATA1 and YY1 are developmental repressor of the human epsilon-globin gene. EMBO J 1995; 14:801-9.
22. Peters B, Merezhinskaya N, Diffley JF et al. Protein-DNA interactions in the epsilon-globin gene silencer. J Biol Chem, 1993; 268:3430-7.
23. Moitreyee CK, Suraksha A, Swarup AS. Potential role of NF-κB and RXR beta like proteins in interferon induced HLA class I and beta globin gene transcription in K562 erythroleukaemia cells. Mol Cell Biochem 1998; 178(1-2):103-12.