Molecular Shuttles Operating Undercover: A New Photolithographic Approach for the Fabrication of Structured Surfaces Supporting Directed Motility

Nano Letters

Volumn 3, Issue 12, 2003, Pages 1651-1655

  Hess, Henry ^a   Matzke, Carolyn M ^b   Doot, Robert K ^a   Clemmens, John ^a   Bachand, George D ^b   Bunker, Bruce C ^b   Vogel, Viola ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b SANDIA NATIONAL LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

KINESIN;

ADSORPTION; ARTICLE; BIOMOLECULAR ELECTRONICS; GEOMETRY; IN VITRO STUDY; INTRACELLULAR TRANSPORT; LIGHT; MICROTUBULE; NANOTECHNOLOGY; PROTEIN TRANSPORT; SURFACE PROPERTY;

EID: 0347362544     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl0347435     Document Type: Article

References (28)

1. Vale, R. D. Cell 2003, 112, 467-480.
2. Hess, H.; Vogel, V. Rev. Mol. Biotechnol. 2001, 82, 67-85.
3. Dennis, J. R.; Howard, J.; Vogel, V. Nanotechnology 1999, 10, 232-236.
4. Hess, H.; Clemmens, J.; Qin, D.; Howard, J.; Vogel, V. Nano Lett. 2001, 1, 235-239.
5. Hess, H.; Clemmens, J.; Howard, J.; Vogel, V. Nano Lett. 2002, 2, 113-116.
6. Hess, H.; Howard, J.; Vogel, V. Nano Lett. 2002, 2, 1113-1115.
7. Hyman, A. A.; Drechsel, D. N.; Kellog, D.; Salser, S.; Sawin, K.; Steffen, P.; Wordeman, L.; Mitchison, T. J. Methods Enzymol. 1991, 196, 478-485.
8. Bohm, K. J.; Stracke, R.; Unger, E. Cell Biol. Int. 2000, 24, 335-341.
9. Suzuki, H.; Oiwa, K.; Yamada, A.; Sakakibara, H.; Nakayama, H.; Mashiko, S. Jpn. J. Appl. Phys. Part 1 1995, 34, 3937-3941.
10. Suzuki, H.; Yamada, A.; Oiwa, K.; Nakayama, H.; Mashiko, S. Biophys. J. 1997, 72, 1997-2001.
11. Nicolau, D. V.; Suzuki, H.; Mashiko, S.; Taguchi, T.; Yoshikawa, S. Biophys. J. 1999, 77, 1126-1134.
12. Bunk, R.; Klinth, J.; Montelius, L.; Nicholls, I. A.; Omling, P.; Tagerud, S.; Mansson, A. Biochem. Biophys. Res. Commun. 2003, 301, 783-788.
13. Hiratsuka, Y.; Tada, T.; Oiwa, K.; Kanayama, T.; Uyeda, T. Q. Biophys. J. 2001, 81, 1555-1561.
14. Moorjani, S. G.; Jia, L.; Jackson, T. N.; Hancock, W. O. Nano Lett. 2003, 3, 633-637.
15. Clemmens, J.; Hess, H.; Howard, J.; Vogel, V. Langmuir 2003, 19, 1738-1744.
16. Clemmens, J.; Hess, H.; Lipscomb, R.; Hanein, Y.; Boehringer, K. F.; Matzke, C. M.; Bachand, G. D.; Bunker, B. C.; Vogel, V., Langmuir, in press.
17. Hess, H.; Clemmens, J.; Matzke, C. M.; Bachand, G. D.; Bunker, B. C.; Vogel, V. Appl. Phys. A 2002, 75, 309-313.
18. Gittes, F.; Mickey, B.; Nettleton, J.; Howard, J. J. Cell Biol. 1993, 120, 923-934.
19. Howard, J.; Hunt, A. J.; Back, S. Methods Cell Biol. 1993, 39, 137-147.
20. Coy, D. L.; Wagenbach, M.; Howard, J. J. Biol. Chem. 1999, 274, 3667-3671.
21. Standard procedure. After assembly, flow cells were filled for 5 min with a 0.5 mg/mL casein solution to precoat the surfaces in order to reduce kinesin denaturation. The casein solution was exchanged against a solution containing 10% kinesin stock solution, 0.1 mM ATP, and 0.02 mg/mL casein in BRB80 buffer. After 5 min, a microtubule solution together with an antifade system (20 mM DTT, 0.02 mg/mL glucose oxidase, 0.008 mg/mL catalase, 20 mM D-glucose) and 0.15 mg/mL casein and 10 μM paclitaxel and 1 mM ATP was introduced for 15 min. To reduce background fluorescence from the microtubule solution, we perfused the flow cell with a wash solution that was identical to the microtubule solution with the exception that the new solution had no microtubules.
22. 4; 1 mM EGTA), 10 μM MgATP, and 0.02 mg/mL casein. After 3 min, unbound kinesin was washed out by perfusing with 20 μL dilution buffer plus 10 μM MgATP. A third perfusion with 20 μL dilution buffer plus 10 μM MgATP, and 0.2 mg/ mL casein was then added for 3 min. Finally, a microtubule solution (20 μM tubulin) based on dilution buffer together with an antifade system (20 mM DTT, 0.02 mg/mL glucose oxidase, 0.008 mg/mL catalase, 20 mM D-glucose), 0.02 mg/mL casein, 10 μM paclitaxel, and 1 mM ATP was introduced. To reduce background fluorescence from the solution, we perfused the flow cell with dilution buffer together with the antifade system, 0.02 mg/mL casein, 10 μM paclitaxel, and 1 mM ATP for the experiments shown in Figures 5 and 6.
23. Stracke, P.; Bohm, K. J.; Burgold, J.; Schacht, H. J.; Unger, E. Nanotechnology 2000, 11, 52-56.
24. 2(D) = c(h) + c(D - h), based on the insight that the dissipation is concentrated on the region between microtubule and surface (see also ref 25).
25. Hunt, A. J.; Gittes, F.; Howard, J. Biophys. J. 1994, 67, 766-781.
26. Caselli, U.; Bertoni-Freddari, C.; Paoloni, R.; Fattoretti, P.; Casoli, T.; Meier-Ruge, W. Gerontology 1999, 45, 307-311.
27. Graf von Keyserlink, D.; Schramm, U. Anat. Anz. 1984, 157, 97-111.
28. Mikelberg, F. S.; Drance, S. M.; Schulzer, M.; Yidegiligne, H. M.; Weis, M. M. Ophthalmology 1989, 96, 1325-1328.