Mechanism Study of dTDP-D-Glucose 4,6-Dehydratase: General Base in Active Site Domain

Journal of Biochemistry and Molecular Biology

Volumn 32, Issue 4, 1999, Pages 358-362

  Sohng, Jae Kyung ^a   Noh, Hyung Rae ^a   Yoo, Jin Cheol ^b  

^a SunMoon University   (South Korea)

^b Chosun University   (South Korea)

Author keywords

dTDP D glucose 4,6 dehydratase; Mechanism; Site directed mutagenesis; UDP D glucose epimerase

Indexed keywords

EID: 0347117804     PISSN: 12258687     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Bechthold, A., Sohng, J. K., Smith, T. M., Chu, X. and Floss, H. G. (1995) Identification of Streptomyces violeceoruber Tü22 genes involved in the biosynthesis of granaticin. Mol. Gen. Genetics 248, 610-620.
2. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
3. Floss, H. G. and Beale, J. M. (1989) Biosynthesis studies on antibiotics Angew. Chem. Int. Ed. Engl. 28, 146-177.
4. Haung H. S. H., Kopecko, D. J. and Baron L. S. (1990) Molecular cloning and physical and functional characterization of the Salmonella typhimurium and Salmonella typhi galactose utilization operons. J. Bacteriol. 172, 4392-4398.
5. Jiang, X.-M., Neal, B., Santiago, F., Lee, S. J., Romana, L. K. and Reeves, P. R. (1991) Structure and sequence of the rfb (O-antigen) gene cluster of Salmonella serovar typhimurium (strain LT2). Mol. Microbiol. 5, 695-713.
6. Kessler, A. C., Haase, A. and Reeves, P. R. (1993) Molecular analysis of the 3,6-dideoxyhexose pathway genes of Yersinia pseudotuberculosis serogroup IIA. J. Bacteriol. 175, 1412-1422.
7. Lemaire H.-G. and Muller-Hill, B. (1986) Nucleotide sequences of the galE gene and the galT gene of E. coli. Nucleic Acids Res. 14, 7705-7711.
8. Linton, K. J., Jarvis, B. W. and Hutchinson, C. R. (1995) Cloning of the genes encoding thymidine diphosphoglucose 4,6-dehydratase and thymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase from the erythromycin-producing Saccharopolyspora erythraea. Gene 153, 33-40.
9. Macpherson D. F., Manning P. A. and Morona, R. (1994) Characterization of the dTDP-rhamnose biosynthetic genes encoded in the rfb locus of Shigella flexneri. Mol. Microbiol. 11, 281-292.
10. Melo, A., Elliott, H. and Glaser, L. (1968) The mechanism of 6-deoxyhexose synthesis J. Biol. Chem. 243, 1467-1474.
11. Merson-Davies, L.A. and Cundliffe, E. (1994) Analysis of five tylosine biosynthetic genes from the tyIIBA region of the Streptomyces fradiae genome. Mol. Microbiol. 13, 349-355.
12. Pissowotzki, K., Mansouri, K. and Piepersberg, W. (1991) Genetics of streptomycin production in Streptomyces griseus: Molecular structure and putative function of genes strELMB2N. Mol. Gen. Genet. 231, 113-123.
13. Robertson, B. D., Frosch, M. and Van Putten, J. P. M. (1993) The role of galE in the biosynthesis and function of gonococcal lipopolysaccharide. Mol. Microbiol. 8. 891-901.
14. Romana, J. K., Santiago, F. S. and Reeves, P. R. (1991) High level expression and purification of dThymidine diphospho-D-glucose 4,6-dehydratase (rfbB) from Salmonella serovar typhimurium LT2. Biochem. Biophys. Res. Commun. 174, 846-852.
15. Sambrook, J., Fritsch, E. F. and Manisatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
16. Sanger, F., Nicklen, S. and Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
17. Snipes, C. E., Brillinger G. U., Sellers L., Mascaro L. and Floss, H. G. (1977) Stereochemistry of the dTDP-glucose oxidoreductase reaction. J. Biol.Chem. 252, 8113-8117.
18. Sohng, J.-K. and Yoo, J.-C. (1996) Cloning, sequencing and expression of dTDP-D-glucose 4,6-dehydratase gene from Streptomyces antibioticus Tü99, a producer of chlorothricin. J. Biochem. Mol. Biol. (formerly Korean Biochem. J.) 29, 183-191.
19. Vara, J. A. and Hutchinson, C. R. (1988) Purification of thymidine-diphospho-D-glucose 4,6-dehydratase from an erythromycin-producing strain of Saccharopolyspora erythraea by high resolution liquid chromatography. J. Biol. Chem. 263, 14992-14995.
20. Walsh, C. (1989) Enzymatic Reaction Mechanism, 1st Ed., W. H. Freeman and Company, pp.347-351.
21. Yu, Y., Russell, R. N., Thorson, J. S., Liu, L.-D. and Liu, H.-W. (1992) Mechanistic studies of the biosynthesis of 3,6-dideoxyhexose in Yersinia pseudotuberculosis. J. Biol. Chem. 267, 5868-5875.