Purification and characterization of a serine protease and chitinases from Paecilomyces lilacinus and detection of chitinase activity on 2D gels

Protein Expression and Purification

Volumn 32, Issue 2, 2003, Pages 210-220

  Khan, Alamgir ^a   Williams, Keith ^a   Molloy, Mark P ^b,c   Nevalainen, Helena ^b  

^a Proteome Systems Ltd   (Australia)

^b MACQUARIE UNIVERSITY   (Australia)

^c PFIZER INC   (United States)

Author keywords

2D gel electrophoresis; Bacitracin Sepharose; Chitinase; In situ chitinase assay; Paecilomyces lilacinus; Protease

Indexed keywords

FUNGI; NEMATODA; PAECILOMYCES; PAECILOMYCES LILACINUS;

EID: 0346936016     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.07.007     Document Type: Article

References (27)

1. P. Jatala, R. Kaltenback, M. Bocangel, Biological control of Meloidogyne incognita acrita and Globodera pallida on potatoes, J. Nematol. 11 (1979) 303 (Abstr).
2. P. Jatala, R. Kaltenback, M. Bocangel, A.J. Devaus, R. Campos, Field application of Paecilomyces lilacinus for controlling Meloidogyne incognita on potatoes, J. Nematol. 12 (1980) 226-227.
3. E. Cabanillas, K.R. Barker, Impact of Paecilomyces lilacinus inoculum level and application time on control of Meloidogyne incognita on tomato, J. Nematol. 21 (1989) 115-120.
4. F.A. Zaki, D.S. Bhatti, Effect of castor (Ricinus communus) and the biocontrol fungus Paecilomyces lilacinus on Meloidogyne javanica, Nematologica 36 (1990) 114-122.
5. D.G. Kim, R.D. Riggs, K.S. Kim, Ultrastructure of Heterodera glycines parasitised by Arkansas Fungus 18, Phytopathology 82 (1992) 429-433.
6. S.L.F. Meyer, W.P. Wergin, Colonisation of soybean cyst nematode females, cysts and gelatinous matrices by the fungus Verticillium lecanii, J. Nematol. 30 (1998) 436-450.
7. P.J.M. Bonants, P.F.L. Fitters, H. Thijs, E. den Belder, C. Waalwijk, J.W.D.M. Henfling, A basic serine protease from Paecilomyces lilacinus with biological activity against Meloidogyne hapla eggs, Microbiology 141 (1995) 775-784.
8. R. Segers, T.M. Butt, B.R. Kerry, A. Beckett, J.F. Peberdy, The role of proteinase VCPI produced by the nematophagous fungus Verticillium chlamydosporium in the infection process of nematode eggs, Mycol. Res. 100 (1996) 421-428.
9. D. Wharton, Nematode eggshells, Parasitology 81 (1980) 447-463.
10. S.C. Gupta, T.D. Leathers, D.T. Wicklow, Hydrolytic enzymes secreted by Paecilomyces lilacinus cultured on sclerotia of Aspergillus flavus, Appl. Microbiol. Biotechnol. 39 (1993) 99-103.
11. J. Trudel, A. Asselin, Detection of chitin deacetylase activity after polyacrylamide gel electrophoresis, Anal. Biochem. 189 (1990) 249-253.
12. A. de Siqueira Pinto, C.C. Barreto, A. Schrank, C.J. Ulhoa, M.H. Vainstein, Purification and characterisation of an extracellular chitinase form the entomopathogen Metarhizium anisopliae, Can. J. Microbiol. 43 (1997) 322-327.
13. B.J. Davis, Disc electrophoresis II. Method and application to human serum proteins, Ann. N. Y. Acad. Sci. 121 (1964) 404-427.
14. R.A. Reisfeld, U.S. Lewis, D.E. Williams, Disk electrophoresis of basic proteins and peptides on polyacrylamide gels, Nature 195 (1962) 281-283.
15. J. Trudel, P. Audy, A. Asselin, Electrophoretic forms of chitinase activity in Xanthi-nc tobacco, healthy and infected with tobacco mosaic virus, Mol. Plant-Microb. Interact. 2 (1989) 315-324.
16. R.E. Lovrien, T. Gusek, B. Hart, Cellulase and protease specific activities of commercially available cellulase preparations, J. Appl. Biochem. 7 (1985) 258-272.
17. R.M.C. Dawson, D.C. Elliott, W.H. Elliott, K.M. Jones, Data for Biochemical Research, Oxford University Press, London, 1969, 592 pp.
18. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.
19. H. Blum, H. Beier, H.J. Gross, Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels, Electrophoresis 8 (1987) 93-99.
20. M.P. Molloy, B.R. Herbert, K.L. Williams, A.A. Gooley, Extraction of Escherichia coli proteins with organic solvents prior to two-dimensional electrophoresis, Electrophoresis 20 (1999) 701-704.
21. H. Towbin, T. Staehelin, J. Gordon, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA 76 (1979) 4350-4354.
22. J. Trudel, A. Asselin, Detection of chitinase activity after polyacrylamide gel electrophoresis, Anal. Biochem. 178 (1989) 362-366.
23. J. Molano, A. Duran, E. Cabib, A rapid and sensitive assay for chitinase using tritiated chitin, Anal. Biochem. 83 (1977) 648-656.
24. M.J. North, Comparative biochemistry of the Eucaryotic microorganisms, Microbiol. Rev. 46 (1982) 308-340.
25. A. Tronsmo, G.E. Harman, Detection and quantitation of N-acetyl-β-D-glucosaminide, chitobiosidase, and endo-chitinase in solutions and on gels, Anal. Biochem. 208 (1993) 74-79.
26. D. Koga, K.J. Kramer, Hydrolysis of glycol chitin by chitinolytic enzymes, Comp. Biochem. Physiol. 76B (1983) 291-293.
27. E.E. Deane, J.M. Whips, J.M. Lynch, J.F. Peberdy, The purification and characterisation of a Trichoderma harzianum exochitinase, Biochim Biophys. Acta 1383 (1998) 101-110.