Generation of an Escherichia coli lysA targeted deletion mutant by double cross-over recombination for potential use in a bacterial growth-based lysine assay

Letters in Applied Microbiology

Volumn 37, Issue 6, 2003, Pages 458-462

  Li, X ^a,b   Ricke, S C ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Escherichia coli; lysA gene; Lysine assay; Targeted deletion mutant

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; GENES;

A-STABLE; AUXOTROPHIC STRAINS; BACTERIAL GROWTH; CROSS-OVER; DELETION MUTANTS; E. COLI; HOMOLOGOUS SEQUENCES; LYSA GENE; LYSINE ASSAY; TARGETED DELETION MUTANT;

ESCHERICHIA COLI;

AMPICILLIN; ANTIBIOTIC AGENT; DNA;

BACTERIUM;

ANTIBIOTIC RESISTANCE; ARTICLE; AUXOTROPHIC MUTANT; DELETION MUTANT; DNA SEQUENCE; ESCHERICHIA COLI; GENE; GENETIC TRANSDUCTION; LYSA GENE; NONHUMAN; POLYMERASE CHAIN REACTION; STRAIN DIFFERENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0346887118     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.2003.01425.x     Document Type: Article

References (13)

1. Adelberg, E.A., Mandel, M. and Chen, G.C.C. (1965) Optimal conditions for mutagenesis by N-methyl-N-nitro-N-nitrosoguandine in Escherichia coli K12. Biochemica and Biophysica Research Communications 18, 788-795.
2. Boy, E., Richaud, C. and Patte, J.-C. (1979) Multiple regulation of DAP-decarboxylase synthesis in Escherichia coli K12. Federation of European Microbiological Societies Microbiological Letters 5, 287-290.
3. Carpenter, K.J. and Booth, V.H. (1973) Damage to lysine in food processing: its measurement and its significance. Nutrition Abstracts & Review 43, 423-451.
4. Erickson, A.M., Li, X., Woodward, C.L. and Ricke, S.C. (1999) Optimisation of enzyme treatment for the degradation of feed proteins for an Escherichia coli auxotroph lysine availability assay. Journal of the Science of Food and Agriculture 79, 1929-1935.
5. Hitchins, A.D., McDonough, F.E. and Wells, P.A. (1989) The use of Escherichia coli mutants to measure the bioavailability of essential amino acids in foods. Plant Foods for Human Nutrition 39, 109-120.
6. Li, X., Erickson, A.M. and Ricke, S.C. (2000) Agitation during incubation reduces the time required for a lysine microbiological growth assay using an Escherichia coli auxotrophic mutant. Journal of Rapid Methods and Automation in Microbiology 8, 83-94.
7. Mekalanos, J.J., Swartz, D.J., Pearson, G.D.N., Harford, N., Groyne, F. and Wilde, M (1983) Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. Nature (London) 306, 551-557.
8. Miller, J.H. (1972) Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory Press.
9. - auxotroph to assay nutritionally available lysine in biological materials. Journal of Applied Bacteriology 42, 165-177.
10. Pitcher, D.G., Saunders, N.A. and Owen, R.J. (1989) Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Letters in Applied Microbiology 8, 151-156.
11. Stragier, P., Richaud, F., Borne, F. and Patte, J.-C. (1983) Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli I. Identification of a lysR gene encoding an activator of the lysA gene. Journal of Molecular Biology 168, 307-320.
12. Tuffnell, J.M. and Payne, J.W. (1985) A colorimetric enzyme assay using Escherichia coli to determine nutritionally available lysine in biological materials. Journal of Applied Bacteriology 58, 333-341.
13. Yu, D., Ellis, H.M., Lee, E.-C., Jenkins, N.A., Copeland, N.G. and Court, D.L. (2000) An efficient recombination system for chromosome engineering in Escherichia coli. Proceedings of the Natlional Academy of Sciences USA 97, 5978-5983.