Identification of two functional nuclear localization signals in DNase γ and their roles in its apoptotic DNase activity

Biochemical Journal

Volumn 376, Issue 2, 2003, Pages 377-381

  Shiokawa, Daisuke ^a   Shika, Yukari ^a   Tanuma, Sei Ichi ^a  

^a TOKYO UNIVERSITY OF SCIENCE   (Japan)

Author keywords

Apoptosis; DNA fragmentation; DNase; Green fluorescent protein; Nuclear localization signal

Indexed keywords

CELLS; DNA;

APOPTOSIS;

BIOCHEMISTRY;

DEOXYRIBONUCLEASE; DNA FRAGMENT; GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; APOPTOSIS; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ANIMALS; APOPTOSIS; CELL LINE; DEOXYRIBONUCLEASE I; DNA FRAGMENTATION; ENDODEOXYRIBONUCLEASES; HUMANS; NUCLEAR LOCALIZATION SIGNALS; RECOMBINANT FUSION PROTEINS;

ANIMALIA;

EID: 0346887110     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030820     Document Type: Article

References (23)

1. Shiokawa, D., Iwamatsu, A. and Tanuma, S. (1997) Purification, characterization, and amino acid sequencing of DNase γ from rat spleen. Arch. Biochem. Biophys. 346, 15-20
2. Shiokawa, D., Ohyama, H., Yamada, T. and Tanuma, S. (1997) Purification and properties of DNase γ from apoptotic rat thymocytes. Biochem. J. 326, 675-681
3. Shiokawa, D. and Tanuma, S. (1998) Molecular cloning and expression of a cDNA encoding an apoptotic endonuclease DNase γ. Biochem. J. 332, 713-720
4. Rodriguez, A. M., Rodin, D., Nomura, H., Morton, C. C., Weremowicz, S. and Schneider, M. C. (1997) Identification, localization, and expression of two novel human genes similar to deoxyribonuclease I. Genomics 42, 507-513
5. Shak, S., Capon, D. J., Hellmiss, R., Marsters, S. A. and Baker, C. L. (1990) Recombinant human DNase I reduces the viscosity of cystic fibrosis sputum. Proc. Natl. Acad. Sci. U.S.A. 87, 9188-9192
6. Pergolizzi, R., Appierto, V., Bosetti, A., DeBellis, G. L., Rovida, E. and Biunno, I. (1996) Cloning of a gene encoding a DNase I-like endonuclease in the human Xq28 region. Gene 168, 267-270
7. Parrish, J. E., Ciccodicola, A., Wehhert, M., Cox, G. F., Chen, E. and Nelson, D. L. (1995) A muscle-specific DNase I-like gene in human Xq28. Hum. Mol. Genet. 4, 1557-1564
8. Shiokawa, D., Hirai, M. and Tanuma, S. (1998) cDNA cloning of human DNase γ: chromosomal localization of its gene and enzymatic properties of recombinant protein. Apoptosis 3, 89-95
9. Shiokawa, D. and Tanuma, S. (2001) Characterization of human DNase I family endonucleases and activation of DNase γ during apoptosis. Biochemistry 40, 143-152
10. Napirei, M., Karsunky, H., Zevnik, B., Stephan, H., Mannherz, H. G. and Moroy, T. (2000) Features of systemic lupus erythematosus in Dnase1-deficient mice. Nat. Genet. 25, 177-181
11. 2+-dependent cleavage of DNA into oligonucleosomal and high molecular mass fragments. Nucleic Acids Res. 27, 1999-2005
12. Boulares, A. H., Zoltoski, A. J., Contreras, F. J., Yakovlev, A. G., Yoshihara, K. and Smulson, M. E. (2002) Regulation of DNAS1L3 endonuclease activity by poly(ADP-ribosyl)ation during etoposide-induced apoptosis: role of PARP-1 cleavage in endonuclease activation. J. Biol. Chem. 277, 372-378
13. Liu, Q. Y., Ribecco, M., Pandey, S., Walker, P. R. and Sikorska, M. (1999) Apoptosis-related functional features of the DNaseI-like family of nucleases. Ann. N.Y. Acad. Sci. 887, 60-76
14. Arends, M. J., Morris, R. G. and Wyllie, A. H. (1990) Apoptosis. The role of the endonuclease. Am. J. Pathol. 136, 593-608
15. Shiokawa, D., Kobayashi, T. and Tanuma, S. (2002) Involvement of DNase γ in apoptosis associated with myogenic differentiation of C2C12 cells. J. Biol. Chem. 277, 31031-31037
16. Shiokawa, D. and Tanuma, S. (1999) DLAD, a novel mammalian divalent cation-independent endonuclease with homology to DNase II. Nucleic Acids Res. 27, 4083-4089
17. Fimia, G. M., Gottifredi, V., Passananti, C. and Maione, R. (1996) Double-stranded internucleosomal cleavage of apoptotic DNA is dependent on the degree of differentiation in muscle cells. J. Biol. Chem. 271, 15575-15579
18. Nishikawa, A., Gregory, W., Frenz, J., Cacia, J. and Kornfeld, S. (1997) The phosphorylation of bovine DNase I Asn-linked oligosaccharides is dependent on specific lysine and arginine residues. J. Biol. Chem. 272, 19408-19412
19. Robbins, J., Dilworth, S. M., Laskey, R. A. and Dingwall, C. (1991) Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell 64, 615-623
20. Chelsky, D., Ralph, R. and Jonak, G. (1989) Sequence requirements for synthetic peptide-mediated translocation to the nucleus. Mol. Cell. Biol. 9, 2487-2492
21. Prunell, A., Kornberg, R. D., Lutter, L., Klug, A., Levitt, M. and Crick, F. H. (1979) Periodicity of deoxyribonuclease I digestion of chromatin. Science 204, 855-858
22. Shiokawa, D., Tanaka, M., Kimura, T., Hashizume, K., Takasawa, R., Ohyama, H., Fujita, K., Yamada, T. and Tanuma, S. (2000) Characterization of two DNase γ-specific monoclonal antibodies and the in situ detection of DNase γ in the nuclei of apoptotic rat thymocytes. Biochem. Biophys. Res. Commun. 275, 343-349
23. Nigg, E. A. (1997) Nucleocytoplasmic transport: signals, mechanisms and regulation. Nature (London) 386, 779-787