Development of a CAPS (cleaved amplified polymorphics sequence) assay for sex identification of the emu (Dromaius novaehollandiae)

Molecular Ecology Notes

Volumn 1, Issue 4, 2001, Pages 273-275

  De Kloet, Siwo R ^a  

^a Avian Biotech Int   (United States)

Author keywords

CAPS; Emu; RAPD; Sex identification

Indexed keywords

EID: 0346835979     PISSN: 14718278     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-8278.2001.00121.x     Document Type: Article

References (17)

1. Ansari HA, Takagi N, Sasaki M (1988) Morphological differentiation of sex chromosomes in three species of ratite birds. Cytogenetics and Cell Genetics, 47, 185-188.
2. Bello N & Sanchez A (1999) The identification of a sex-specific DNA marker in the ostrich using a random amplified polymorphic DNA (RAPD) assay. Molecular Ecology, 8, 667-669.
3. Blanck AB, Glück RS, Wartbichler S, Bender MA, Pöll A, Brandl A (1997) Activity of Restriction Enzymes in a PCR Mix. Biochemica (Boehringer), 3, 25.
4. Ellegren H (1996) First gene on the avian W-chromosome (CHD) provides a tag for universal sexing of non-ratite birds. Proceedings of the Royal Society London Series B, Biological Sciences, 1377, 1635-1641.
5. Fridolfsson A-K, Cheng H, Copeland NG et al. (1998) Evolution of the avian sex chromosomes from an ancestral pair of autosomes. Proceedings of the National Academy of Sciences of the USA, 95, 8147-8152.
6. Griffiths R, Double MC, Orr K, Dawson DJC (1998) A DNA test to sex most birds. Molecular Ecology, 7, 1071-1075.
7. Gustincich S, Manfioletti G, Del Sal GC, Schneider C, Carnici PC (1991) A fast method for high-quality genomic DNA extraction from whole human blood. Biotechniques, 11, 298-302.
8. Lessells CM & Mateman AC (1998) Sexing birds using random amplified polymorphic DNA (RAPD) markers. Molecular Ecology, 7, 187-195.
9. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: a Laboratory Manual. Cold Spring Harbour Laboratory Press, New York.
10. Meunier JR & Grimont PAD (1993) Factors affecting reproducibility of amplified polymorphic DNA fingerprints. Research in Microbiology, 144, 373-379.
11. Ogawa A, Murata K, Mizuno S (1998) Location of Z- and W-linked marker genes and sequence on homomorphic sex chromosomes of the ostrich and the emu. Proceedings of the National Academy of Sciences of the USA, 95, 4415-4418.
12. Ogawa A, Solovei I, Hutchison N et al. (1997) Molecular characterization and cytological mapping of a non-repetitive DNA sequence region from the W-chromosome of chicken and the use as a universal probe for sexing Carinatae birds. Chromosome Research, 5, 93-101.
13. Parry HD & Alphey L (1995) The utilization of cloned DNAs. In: DNA Cloning: 1 Core Techniques (eds Glover DM, Hames BD), pp. 143-191. Oxford University Press, Oxford.
14. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the USA, 74, 5463-5467.
15. Sean Walsh P, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques, 10, 506-513.
16. Taberlet P & Bouvet J (1991) A single plucked feather as a source of DNA for bird genetic studies. Auk, 108, 959-960.
17. Williams JGK, Kubelik AR, Livak KJ, Rafalskia JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18, 6531-6535.