Purification and characterization of two anionic trypsins from the hepatopancreas of carp

Fisheries Science

Volumn 66, Issue 6, 2000, Pages 1172-1179

  Cao, Min Jie ^a   Osatomi, Kiyoshi ^a   Suzuki, Miho ^a   Hara, Kenji ^a   Tachibana, Katsuyasu ^a   Ishihara, Tadashi ^a  

^a NAGASAKI UNIVERSITY   (Japan)

Author keywords

Amino acid sequence; Carp; Hepatopancreas; Immunoblotting; Purification; Serine proteinase; Trypsin

Indexed keywords

EID: 0346764650     PISSN: 09199268     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1444-2906.2000.00185.x     Document Type: Article

References (16)

1. Brodrick JW, Largman C, Geokas MC, O'Rourke M, Ray SB. Clearance of circulating anionic and cationic pancreatic trypsinogens in the rat. Am. J. Physiol. 1980; 239: 511-515.
2. Pinsky SD, Laforge KS, Scheele G. Differential regulation of trypsinogen mRNA translation: Full-length mRNA sequences encoding two oppositely charged trypsinogen isoenzymes in the dog pancreas. Mol. Cell. Biol. 1985; 5: 2669-2676.
3. Mikes O, Holeysovsky V, Tomasek V, Sorm E Covalent structure of bovine trypsinogen. The position of the remaining amides. Biochem. Biophys. Res. Commun. 1966; 24: 346-352.
4. LaVallie ER, Rehemtulla A, Racie LA, DiBlasio EA, Ferenz C, Grant KL, Light A, McCoy JM. Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase. J. Biol. Chem. 1993; 268: 23311-23317.
5. Tsuchiya Y, Takahashi T, Sakurai Y, Iwamatsu A, Takahashi K. Purification and characterization of a novel membrane-bound arginine-specific serine proteinase from porcine intestinal mucosa. J. Biol. Chem. 1994; 269: 32985-32991.
6. Jeohn GH, Serizawa S, Iwamatsu A, Takahashi K. Isolation and characterization of gastric trypsin from the microsomal fraction of porcine gastric antral mucosa. J. Biol. Chem. 1995; 270: 14748-14755.
7. Leytus SP, Loeb KR, Hagen FS, Kurachi K, Davie EW. A novel trypsin-like serine protease (Hepsin) with a putative transmembrane domain expressed by human liver and hepatoma cells. Biochemistry 1988; 27: 1067-1074.
8. Murakami K, Noda M. Studies on proteinases from the digestive organs of sardine - I. Purification and characterization of three alkaline proteinases from the pyloric caeca. Biochim. Biophys. Acta 1981; 658: 17-26.
9. Hjelmeland K, Raa J. Characteristics of two trypsin type isozymes isolated from the Arctic fish capelin (Mallottu villosus). Comp. Biochem. Physiol. 1982; 71B: 557-562.
10. Yoshinaka R, Suzuki T, Sato M, Ikeda S. Purification and some properties of anionic trypsin from the catfish pancreas. Nippon Suisan Gakkaishi 1983; 49: 207-212.
11. Yoshinaka R, Sato M, Suzuki T, Ikeda S. Enzymatic characterization of anionic trypsin of the catfish (Parasilurus asotus). Comp. Biochem. Physiol. 1984; 77B: 1-6.
12. Simpson BK, Haard NF. Purification and characterization of trypsin from the Greenland cod (Gadus ogac). 1. Kinetic and thermodynamic characteristics. Can. J. Biochem. Cell. Biol. 1984a; 62: 894-900.
13. Simpson BK, Haard NF. Trypsin from the Greenland cod, Gadus ogac. Isolation and comparative properties. Comp. Biochem. Physiol. 1984; 79B: 613-622.
14. Asgeirsson B, Fox JW, Bjamason JB. Purification and characterization of trypsin from the poikilotherm Gadus morhua. Eur. J. Biochem. 1989; 180: 85-94.
15. Martinez A, Olsen RL, Serra JL. Purification and characterization of two trypsin-like enzymes from the digestive tract of anchovy Engraulis encrasicholus. Comp. Biochem. Physiol. 1988; 91B: 677-684.
16. Cohen T, Gertler A, Birk Y. Pancreatic proteolytic enzymes from carp (Cyprinus carpio - I. Purification and physical properties of trypsin, chymotrypsin, elastase and carboxypeptidase B. Comp. Biochem. Physiol. 1981; 69B: 639-646.