Automated Analysis of Morphometric Parameters for Accurate Definition of Erythrocyte Cell Shape

Cytometry Part A

Volumn 52, Issue 1, 2003, Pages 12-18

  Albertini, Maria C ^a   Teodori, Laura ^b   Piatti, Elena ^a   Piacentini, Maria P ^a   Accorsi, Augusto ^a   Rocchi, Marco B L ^a  

^a UNIVERSITY OF URBINO   (Italy)

^b ENEA C R CASACCIA   (Italy)

Author keywords

Automated analysis; Cup shaped cells; Echinocytes; Erythrocyte morphology; Macrocytes; Microcytes; Morphometric parameters; NIH Scion Image; Ovalocytes; Statistical multivariate discriminant analysis; Target cells

Indexed keywords

ARTICLE; AUTOANALYSIS; CELL STRUCTURE; CELL TYPE; COMPUTER PROGRAM; DISCRIMINANT ANALYSIS; ERYTHROCYTE SHAPE; HUMAN; HUMAN CELL; IMAGE PROCESSING; IN VITRO STUDY; IN VIVO STUDY; MEGALOCYTE; MICROCYTE; MICROSCOPY; NORMAL HUMAN; OVALOCYTE; PRIORITY JOURNAL; TARGET CELL;

EID: 0346756192     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.10019     Document Type: Article

References (19)

1. Turchetti V, De Matteis C, Leoncini F, Trabalzini L, Guerrini M, Forconi S. Variations of erythrocyte morphology in different pathologies. Clin Hemorheol Microcirc 1997;17:209-215.
2. Bossi D, Russo M. Hemolytic anemias due to disorders of red cell membrane skeleton. Mol Aspects Med 1996;17:171-188.
3. Agroyannis B, Dalamangas A, Tzanatos H, Soubassi L, Fourtounas C, Kopelias I, Bovoleti O, Bosiolis V, Koutsikos D. Alterations in echinocyte transformation and erythrocyte sedimentation rate during hemodialysis. Artif Organs 1997;21:327-330.
4. Nakao M, Jinbu Y, Sato S, Ishigami Y, Nakato T, Ito Ueno E, Wake K. Structure and function of red cell cytoskeleton. Biomed Biochim Acta 1987;46:S5-S9.
5. Marikovsky Y. The cytoskeleton in ATP-depleted erythrocytes: the effect of shape transformation. Mech Ageing Dev 1996;86:137-144.
6. Thimon L, Peypoux F, Exbrayat JM, Michel G. Effect of iturin A, a lipopeptide from Bacillus subtilis on morphology and ultrastructure of human erythrocytes. Cytobios 1994;79:69-83.
7. Yawata Y, Kanzaki A, Inoue T, Ata K, Wada H, Okamoto N, Higo I, Yawata A, Sugihara T, Yamada O. Red cell membrane disorders in the Japanese population: clinical, biochemical, electron microscopic, and genetic studies. Int J Hematol 1994;60:23-38.
8. Grebe R, Schmid Schonbein H. Tangent counting for objective assessment of erythrocyte shape changes. Biorheology, 1985;22:455-469.
9. Turchetti V, Leoncini F, De Matteis C, Trabalzini L, Guerrini M, Forconi S. Evaluation of erythrocyte morphology as deformability index in patients suffering from vascular diseases, with or without diabetes mellitus: correlation with blood viscosity and intra-erythrocytic calcium. Clin Hemorheol Microcirc 1998;18:141-149.
10. Zipursky A, Brown E, Palko J, Browna EJ. The erythrocyte differential count in newborn infants. Aro J Pediatr Hematol Oncol 1983;5:45-51.
11. Bessis M. Cellules du sang normal et pathologiques. Paris: Masson et Cie; 1972.
12. Anderson TW. An introduction to multivariate statistical analysis. New York: John Wiley & Sons; 1984.
13. Cohen J. A coefficient of agreement for nominal scales. Educ Psychol Meas 1960;20:37-46.
14. Landis RJ, Koch GG. The measurement of observer agreement for categorical data. Biometrics 1977;33:159-174.
15. Fleiss JL, Cohen J, Everitt BS. Large sample standard errors of kappa and weighted kappa. Psycol Bull 1969;72:323-327.
16. Eldibany M, Totonchi K, Joseph N, Rhone D. Usefulness of certain red blood cell indices in diagnosis and differentiating thalassemia trait from iron-deficiency anemia. Am J Clin Pathol 1999;111:676-682.
17. Scion Corporation. Scion products. Frederick, MD: Scion Corporation. Updated 1 October 2002. Available from: http://scioncorp.com/frames/fr_scion_products.htm
18. Hung TC, Pham S, Steed DL, Webster MW, Butter DB. Alterations in erythrocyte rheology in patients with severe peripheral vascular disease. Angiology 1991;42:210-217.
19. Ayala S, Besson I, Aymerich M, Berga L, Vives Corrons JL. Abnormal changes in erythrocyte membrane proteins in hereditary spherocytosis and their relation to clinical and biological aspects of the disease. Med Clin Barc 1995;105:45-49.