A SYBR green, real-time RT-PCR method to detect and quantitate Norwalk virus in stools

Journal of Virological Methods

Volumn 116, Issue 1, 2004, Pages 63-70

  Richards, Gary P ^a   Watson, Michael A ^a   Kingsley, David H ^a  

^a DELAWARE STATE UNIVERSITY   (United States)

Author keywords

Assay; Extraction; Norovirus; Real time RT PCR; Stool; Volunteer

Indexed keywords

VIRUS RNA;

AMPLICON; ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; DILUTION CURVE; FECES ANALYSIS; FECES CULTURE; FLUORESCENCE ANALYSIS; HUMAN; NONHUMAN; NORWALK GASTROENTERITIS VIRUS; PRIORITY JOURNAL; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS CARRIER; VIRUS DETECTION;

NOROVIRUS; NORWALK VIRUS;

EID: 0346727186     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2003.10.011     Document Type: Article

References (19)

1. Arnal C., Ferre-Aubineau V., Besse B., Mignotte B., Schwartzbrod L., Billaudel S. Comparison of seven RNA extraction methods on stool and shellfish samples prior to hepatitis A virus amplification. J. Virol. Methods. 77:1999;17-26.
2. Chen Y., Mao J., Hong Y., Yang L., Ling Z., Yu W. Genetic analysis of wild-type hepatitis A virus strains. Chin. Med. J. (English). 114:2001;422-423.
3. Cliver D.O. Virus transmission via food. Food Technol. 51:1997;71-78.
4. Graham D.Y., Jiang X., Tanaka T., Opekun A.R., Madore H.P., Estes M.K. Norwalk virus infection of volunteers: new insights based on improved assays. J. Infect. Dis. 170:1994;34-43.
5. Hale A.D., Green J., Brown D.W. Comparison of four RNA extraction methods for the detection of small round structured viruses in faecal specimens. J. Virol. Methods. 57:1996;195-201.
6. Iversen A.M., Gill M., Bartlett C.L., Cubitt W.D., McSwiggan D.A. Two outbreaks of foodborne gastroenteritis caused by a small round structured virus: evidence of prolonged infectivity in a food handler. Lancet. 2(8558):1987;556-558.
7. Kapikian A.Z., Wyatt R.G., Dolin R., Thornhill T.S., Kalica A.R., Chanock R.M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J. Virol. 10:1972;1075-1081.
8. Kageyama T., Kojima S., Shinohara M., Uchida K., Fukushi S., Hoshino F.B., Takeda N., Katayama K. Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR. J. Clin. Microbiol. 41:2003;1548-1557.
9. Kok T., Wati S., Bayly B., Devonshire-Gill D., Higgins G. Comparison of six nucleic acid extraction methods for detection of viral DNA or RNA sequences in four different non-serum specimen types. J. Clin. Virol. 16:2000;59-63.
10. Matsui S.M., Kim J.P., Greenberg H.B., Su W., Sun Q., Johnson P.C., Dupont H.L., Oshiro L.S., Reyes G.R. The isolation and characterisation of a Norwalk virus-specific cDNA. J. Clin. Invest. 87:1991;1456-1461.
11. Mead P.S., Slutsker L., Dietz V., McCaig L.F., Bresee J.S., Chapiro C., Griffin P.M., Tauxe R.V. Food-related illness and deaths in the United States. Emerg. Infect. Dis. 5:1999;607-625.
12. Mendez I.I., Hermann L.L., Hazelton P.R., Coombs K.M. A comparative analysis of freon substitutes in the purification of reovirus and calicivirus. J. Virol. Methods. 90:2000;59-67.
13. Muir P., Nicholson F., Jhetam M., Neogi S., Banatvala J.E. Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens. J. Clin. Microbiol. 31:1993;31-38.
14. Queiroz A.P., Santos F.M., Sassaroli A., Harsi C.M., Monezi T.A., Mehnert D.U. Electropositive filter membrane as an alternative for the elimination of PCR inhibitors from sewage and water samples. Appl. Environ. Microbiol. 67:2001;4614-4618.
15. Rasool N.B., Monroe S.S., Glass R.I. Determination of a universal nucleic acid extraction procedure for PCR detection of gastroenteritis viruses in faecal specimens. J. Virol. Methods. 100:2002;1-16.
16. Richards G.P. Limitations of molecular biological techniques for assessing the virological safety of foods. J. Food Prot. 62:1999;691-697.
17. Schwab K.J., Estes M.K., Neill F.H., Atmar R.L. Use of heat release and an internal RNA standard control in reverse transcription-PCR detection of Norwalk virus from stool samples. J. Clin. Microbiol. 35:1997;511-514.
18. White K.E., Osterholm M.T., Mariotti J.A., Korlath J.A., Lawrence D.H., Ristinen T.L., Greenberg H.B. A foodborne outbreak of Norwalk virus gastroenteritis: evidence for post recovery transmission. Am. J. Epidemiol. 124:1986;120-126.
19. Wilde J., Eiden J., Yolken R. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reaction. J. Clin. Microbiol. 28:1990;1300-1307.