Freshwater phytoplankton quantification by chlorophyll a: A comparative study of in vitro, in vivo and in situ methods

Water Research

Volumn 38, Issue 3, 2004, Pages 517-522

  Gregor, J ^a   Marsalek B ^a  

^a INSTITUTE OF BOTANY   (Czech Republic)

Author keywords

Algal blooms; Chlorophyll a; Fluorescence; Phytoplankton quantification; Submersible FluoroProbe; Water quality

Indexed keywords

ALGAE; BACTERIA; BIODIVERSITY; CHLOROPHYLL; FLUORESCENCE; POTABLE WATER; RESERVOIRS (WATER); RIVERS;

SPECTROFLUOROMETRY;

WATER ANALYSIS;

CHLOROPHYLL A;

ALGAL BLOOM; CHLOROPHYLL A; FLUORESCENCE SPECTROSCOPY; IN SITU MEASUREMENT; PHYTOPLANKTON; WATER QUALITY;

ABSORPTION; ANABAENA; ARTICLE; CHLOROPHYLL FLUORESCENCE; CONTROLLED STUDY; CORRELATION ANALYSIS; CYANOBACTERIUM; EXTRACTION; FRESHWATER ENVIRONMENT; MICROCYSTIS; MOLECULAR PROBE; NONHUMAN; PHYTOPLANKTON; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SPECTROFLUOROMETRY; SPECTROPHOTOMETRY; WATER MANAGEMENT;

ALGAE; ANABAENA; APHANIZOMENON; BACILLARIOPHYTA; CHLOROPHYTA; CRYPTOPHYTA; CYANOBACTERIA; MICROCYSTIS;

EID: 0346687724     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.watres.2003.10.033     Document Type: Article

References (19)

1. Richards F.A., Thompson T.G. The estimation and characterization of planktonic populations by pigment analysis. II. A spectrophotometric method for the estimation of plankton pigments. J Marine Res. 11:1952;156-172.
2. Parsons T.R., Strickland J.D.H. Discussion of spectrophotometric determination of marine pigments with revised equations for ascertaining chlorophylls and carotenoids. J Mar Res. 21:1963;155-163.
3. Lorenzen C.J. Determination of chlorophyll and phaeopigments. spectrophotometric equations Limnol Oceanogr. 12:1967;243-246.
4. Holm-Hansen O.C.J., Lorenzen C.J., Holmes R.W., Strickland J.D.H. Fluorometric determination of chlorophyll. J Cons Perm Int Explor Mer. 30:1965;3-15.
5. Jacobsen T.R. A quantitative method for the separation of chlorophyll a and b from phytoplankton pigments by HPLC. Mar Sci Comm. 4:1978;33-47.
6. Otsuki A., Takamura N. Comparison of chlorophyll a concentrations measured by fluorometric HPLC and spectrophotometric methods in highly eutrophic small Lake Kasumiagura. Verh int Ver Limnol. 23:1987;944-951.
7. Beutler M., Wiltshire K.H., Meyer B., Moldaenke C., Luring C., Meyerhofer M., Hansen U.P., Dau H. A fluorometric method for the differentiation of algal populations in vivo and in situ. Photosynth Res. 72(1):2002;39-53.
8. Geis S.W., Fleming K.L., Korthals E.T., Searle G., Reynolds L., Karner D.A. Modifications to the algal growth inhibition test for use as a regulatory assay. Environ Toxicol Chem. 19(1):2000;36-41.
9. Eisentraeger A., Dott W., Klein J., Hahn S. Comparative studies on algal toxicity testing using fluorometric microplate and Erlenmayer flask growth-inhibition assays. Ecotox Environ Safe. 54(3):2003;346-354.
10. Pinto A.M.F., Von Sperling E., Moreira R.M. Chlorophyll-a determination via continuous measurement of plankton fluorescence. methodology development Water Res. 35(16):2001;3977-3981.
11. Odate T., Hirawake T., Kudoh S., Klein B., LeBlanc B., Fukuchi M. Temporal and spatial patterns in the surface-water biomass of phytoplankton in the North Water. Deep-sea Res II. 49:2002;4947-4958.
12. Asai R., Horiguchi Y., Yoshida A., McNiven S., Tahira P., Ikebukuro K., Uchiyama S., Masuda Y., Karube I. Detection of phycobilin pigments and their seasonal change in Lake Kasumigaura using a sensitive in situ fluorometric sensor. Anal Lett. 34(14):2001;2521-2533.
13. Desiderio R.A., Moore C., Lantz C., Cowles T.J. Multiple excitation fluorometer for in situ oceanographic applications. Appl Optics. 36(6):1997;1289-1296.
14. Millie D.F., Schofield O.M.E., Kirkpatrick G.J., Johnsen G., Evens T.J. Using absorbance and fluorescence spectra to discriminate microalgae. Eur J Phycol. 37(3):2002;313-322.
15. Leboulanger C., Dorigo U., Jacquet S., Le Berre B., Paolini G., Humbert J.F. Application of a submersible spectrofluorometer for rapid monitoring of freshwater cyanobacterial blooms. a case study Aquat Microb Ecol. 30(1):2002;83-89.
16. ISO 10260:1992. Water quality - measurement of biochemical parameters - spectrometric determination of the chlorophyll - a concentration. International Organization for Standardization, Geneva, Switzerland, 1992.
17. Meyns S., Illi R., Ribi B. Comparison of chlorophyll-a analysis by HPLC and spectrophotometry. where do the differences come from? Arch Hydrobiol. 132(2):1994;129-139.
18. Strickland J.D.H., Parsons T.R. Pigment analysis - a practical handbook of seawater analysis. Bull Fish Res Board Can. 167:1968;185-205.
19. Jacobsen T.R., Rai H. Comparison of spectrophotometric, fluorometric and HPLC methods for determination of chlorophyll a in aquatic samples. effects of solvent and extraction procedures Int Revue ges Hydrobiol. 75(2):1990;207-217.