Effectiveness and limitations of uracil-DNA glycosylases in sensitive real-time PCR assays

BioTechniques

Volumn 36, Issue 1, 2004, Pages 44-48

  Pierce, Kenneth E ^a   Wangh, Lawrence J ^a  

^a BRANDEIS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA GLYCOSYLTRANSFERASE; URACIL;

ARTICLE; CYTOLYSIS; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME DEGRADATION; FLUORESCENCE; GENE AMPLIFICATION; POLYMERASE CHAIN REACTION;

EID: 0346655256     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04361bm04     Document Type: Article

References (6)

1. Longo, M.C., M.S. Berninger, and J.L. Hartley. 1990. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene 93: 125-128.
2. Espy, M.J., T.F. Smith, and D.H. Persing. 1993. Dependence of polymerase chain reaction product inactivation protocols on amplicon length and sequence composition. J. Clin. Microbiol. 31:2361-2365.
3. Pierce, K.E., J.E. Rice, J.A. Sanchez, C. Brenner, and L.J. Wangh. 2000. Real-time PCR using molecular beacons for accurate detection of the Y chromosome in single human blastomeres. Mol. Hum. Reprod. 6: 1155-1164.
4. Thornton, C.G., J.L. Hartley, and A. Rashtchian. 1992. Utilizing uracil DNA glycosylase to control carryover contamination in PCR: characterization of residual UDG activity following thermal cycling. BioTechniques 13:180-184.
5. Jaeger, S., R. Schmuck, and H. Sobek. 2000. Molecular cloning, sequency, and expression of the heat-labile uracil-DNA glycosylase from a marine psychrophilic bacterium, strain BMTU3346. Extremophiles 4:115-122.
6. Sobek, H., M. Schmidt, B. Frey, and K. Kaluza. 1996. Heat-labile uracil-DNA glycosylase: purification and characterization. FEBS Lett 388:1-4.