Hybridization of Modified-Heme Reconstitution and Distal Histidine Mutation to Functionalize Sperm Whale Myoglobin

Journal of the American Chemical Society

Volumn 126, Issue 2, 2004, Pages 436-437

  Sato, Hideaki ^a,b   Hayashi, Takashi ^a,b   Ando, Tsutomu ^b   Hisaeda, Yoshio ^b   Ueno, Takafumi ^c   Watanabe, Yoshihito ^c  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

^c NAGOYA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

HEMOPROTEIN; HISTIDINE; MYOGLOBIN;

AMINO ACID COMPOSITION; ARTICLE; BINDING SITE; CATALYSIS; HYBRIDIZATION; HYDROPHOBICITY; PROTEIN MODIFICATION; TURNOVER TIME; ULTRAVIOLET SPECTROSCOPY;

ANIMALS; HEME; HISTIDINE; KINETICS; MUTATION; MYOGLOBIN; PEROXIDASE; WHALES;

EID: 0346456937     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja038798k     Document Type: Article

References (27)

1. Turano, P.; Li, Y. In Handbook on Metalloproteins: Bertini, I., Sigel, A., Sigel, H., Eds.: Marcel Dekker: New York. 2001; pp 269-356.
2. (a) Raven, E. L.; Mauk, A. G. In Advances in Inorganic Chemistry; Sykes, A. G., Mauk, G., Eds.; Academic Press: San Diego, 2000: Vol. 51, pp 1-49.
3. (b) Ozaki, S.; Roach, M. P.; Matsui, T.; Watanabe, Y. Acc. Chem. Res. 2001, 34, 818-825.
4. (c) Lei, Y.; Berry, S. M.; Pfister, T. D. Chem. Rev. 2001, 101, 3047-3080 and references therein.
5. (a) Matsui, T.; Ozaki, S.; Watanabe, Y. J. Am. Chem. Soc. 1999, 121, 9952-9957.
6. (b) Matsui, T.; Ozaki, S.; Liong, E.; Phillips, G. N., Jr.; Watabe, Y. J. Biol. Chem. 1999, 274, 2838-2844.
7. (c) Ozaki, S.; Hara, I.; Matsui, T.; Watanabe, Y. Biochemistry 2001, 40, 1044-1052.
8. (a) Rao, S. I.; Wilks, A.; Ortiz de Montellano, P. R. J. Biol. Chem. 1993, 268, 803-809.
9. (b) Levinger, D. C.; Stevenson J.-A.; Wong, L.-L. J. Chem. Soc., Chem. Commun. 1995, 2305-2306.
10. (c) Wan, L.; Twitchett, M. B.; Ellis, L. D.; Mauk, A. G.; Smith, M. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 12825-12831.
11. (d) Sigman, J. A.; Kwok, B. C.; Lei, Y. J. Am. Chem. Soc. 2000, 122, 8192-8196.
12. (a) Hayashi, T.; Hisaeda, Y. Acc. Chem. Res. 2002, 35, 35-43.
13. (b) Hayashi, T.; Hitomi, Y.; Ando, T.; Mizutani, T.; Hisaeda, Y.; Kitagawa, S.; Ogoshi, H. J. Am. Chem. Soc. 1999, 121, 7747-7750.
14. (c) Hayashi, T.; Matsuda, T.; Hisaeda, Y. Chem. Lett. 2003, 32, 496-497.
15. (a) DiNello, R. K.; Dolphin, D. H., J. Biol. Chem. 1981, 256, 6903-6912.
16. (b) Ryabov, A. D.; Goral, V. N.; Gorton, L.; Csöregi, E. Chem. Eur. J. 1999, 5, 961-967.
17. (c) Hamachi, I.; Shinkai, S. Eur. J. Org. Chem. 1999, 1999, 539-549.
18. (d) Monzani, E.; Alzuet, G.; Casella, L.; Redaelli, C.; Bassani, C.; Sanangelantoni, A. M.; Gullotti, M.; Gioia, L. D.; Santagostini, L.; Chillemi, F. Biochemistry 2000, 39, 9571-9582.
19. (a) Teale, F. W. J. Biochim. Biophys. Acta 1959, 35, 543.
20. (b) Asakura, T.; Yonetani, T J. Biol. Chem. 1969, 244, 4573-4579.
21. (c) Hayashi, T.; Hitomi, Y.; Ogoshi, H. J. Am. Chem. Soc. 1998, 120, 4910-4915.
22. Distal histidine mutation to alanine or aspartate was selected to expand the substrate binding pocket and/or facilitate the heme reactive intermediate formation (ref 3a).
23. Chance, B.; Maehly, A. C. Methods Enzymol. 1955, 2, 764-775.
24. DePillis, G. D.; Sishta, B. P.; Mauk, A. G.; Ortiz de Montellano, P. R. J. Biol. Chem. 1991, 266, 19334-19341.
25. -1) was further decreased.
26. -1). For only rMb(H64D·2), a "burst" kinetics was observed due to the slow product release, relating to the high affinity of the product to the active site (data not shown).
27. Savenkova, M. I.; Kuo, J. M.; Ortiz de Montellano, P. R. Biochemistry 1998, 37, 10828-10836.