Application of differential display, with in situ hybridization verification, to microscopic samples of breast cancer tissue

International Journal of Experimental Pathology

Volumn 84, Issue 5, 2003, Pages 207-212

  Kao, Ruey Ho ^a   Francia, Giulio ^b   Poulsom, Richard ^c   Hanby, Andrew M ^d   Hart, Ian R ^b  

^a BUDDHIST TZU CHI GENERAL HOSPITAL   (Taiwan)

^b ST THOMAS HOSPITAL   (United Kingdom)

^c IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^d GUY S HOSPITAL   (United Kingdom)

Author keywords

Breast cancer; Differential display; Gene expression; Heat shock cognate 70; In situ hybridization; Microdissection

Indexed keywords

COMPLEMENTARY DNA;

ARTICLE; BREAST; BREAST CANCER; BREAST DUCT; CANCER DIAGNOSIS; CANCER INFILTRATION; CANCER INVASION; CANCER STAGING; CASE REPORT; CELL TYPE; DIFFERENTIAL DISPLAY; DISEASE SEVERITY; GENE EXPRESSION PROFILING; GENE OVEREXPRESSION; GENE PROBE; HUMAN; HUMAN TISSUE; IN SITU HYBRIDIZATION; MICRODISSECTION; PRIORITY JOURNAL; UPREGULATION;

BASE SEQUENCE; BREAST NEOPLASMS; CARCINOMA, DUCTAL, BREAST; FEMALE; GENE EXPRESSION PROFILING; HSC70 HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; HUMANS; IN SITU HYBRIDIZATION; MICRODISSECTION; MOLECULAR SEQUENCE DATA; NEOPLASM PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, NEOPLASM; UP-REGULATION;

EID: 0346373692     PISSN: 09599673     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2613.2003.00356.x     Document Type: Article

References (19)

1. ANSARI-LARI M.A., JONES S.N., TIMMS K.M. & GIBBS R.A. (1996) Microdissection RT-PCR analysis of gene expression in pathologically defined frozen tissue sections. BioTechniques 21, 38-44.
2. BAKKENIST C.J., KORETH J., WILLIAMS C.S.M., HUNT N.C.A. & MCGEE J.O. (1999) Heat shock cognate 70 mutations in sporadic breast carcinoma. Cancer Res. 59, 4219-4221.
3. DWONRNICZAK B. & MIRAULT M.E. (1987) Structure and expression of a human gene coding for a 71 kd heat shock "cognate" protein. Nucleic Acids Res. 15, 5158-5197.
4. FRANCIA G., MITCHELL S.D., MOSS S.E., HANBY A.M., MARSHALL J.F. & HART I.R. (1996) Identification by differential display of Annexin-VI: a gene differentially expressed during melanoma progression. Cancer Res. 56, 55-58.
5. HARTL F.U. (1996) Molecular chaperones in cellular protein folding. Nature 381, 571-580.
6. HINDS P.W., FINLAY C.A., FREY A.B. & LEVINE A.V. (1987) Immunological evidences for the association of p53 with a heat shock protein, Hsc70, in p53-plus-ras-transformed cell lines. Mol. Cell Biol. 7, 2863-2869.
7. HÖHFELD J. & JENTSCH S. (1997) GrpE-like regulation of the Hsc70 chaperone by the anti-apoptotic protein BAG-1. EMBO J. 16, 6209-6216.
8. KAO R.H., VON SCHUPPE M., FRANCIA G., POWELL J. & HART I.R. (1999) Identification of changes in gene expression associated with minimal residual disease. Cancer Metastasis Rev. 18, 3-13.
9. LIANG P. (2002) A decade of differential display. BioTechniques 33, 338-346.
10. LIANG P. & PARDEE A.B. (1992) Differential display of eukaryotic mRNA by means of the polymerase chain reaction. Science 257, 967-971.
11. NATIONAL STATISTICS. (2001) Cancer trends in England and Wales 1950-1999. SMPS No. 66 The Stationery Office.
12. NIHEI T., TAKAHASHI S., SAGAE S., SATO N. & KIKUCHI K. (1993) Protein interaction of retinoblastoma gene product pRb110 with Mr 73,000 heat shock cognate protein. Cancer Res. 53, 1702-1705.
13. NOCITO A., BUBENDORF L., MARIA TINNER E. ET AL. (2001) Microarrays of bladder cancer tissue are highly representative of proliferation index and histological grade. J. Pathol. 194, 349-357.
14. PARKIN D.M. (1989) Cancers of the breast, endometrium and ovary: geographic correlations. Eur. J. Cancer Clin. Oncol. 25, 1917-1925.
15. PEROU C.M., JEFFREY S.S., VAN DE RIJIN M. ET AL. (1999) Distinctive gene expression patterns in human mammary epithelial cells and breast cancers. Proc. Natl. Acad. Sci. USA 96, 9212-9217.
16. POULSOM R., LONGCRAFT J.M., JEFFERY R.M., ROGERS L. & STEEL J.H. (1998) A robust method for isotopic in situ hybridisation to localize mRNAs in routine pathology specimens. Eur. J. Histochem. 42, 143-150.
17. PRATT W.B. & WELSH M.J. (1994) Chaperone functions of the heat shock proteins associated with steroid receptors. Cell Biol. 5, 83-93.
18. SLAMON D.J., LEYLAND-JONES B., SHAK S. ET AL. (2001) Use of chemotherapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2. N. Engl. J. Med. 344, 783-792.
19. VAN'TVEER L.J., DAI H., VAN DE VIJVER M.J. ET AL. (2002) Gene expression profiling predicts clinical outcome of breast cancer. Nature 415, 530-536.