Transformation of Chlamydomonas reinhardtii CW-15 with the Hygromycin Phosphotransferase Gene as a Selectable Marker

Russian Journal of Genetics

Volumn 38, Issue 9, 2002, Pages 1009-1014

  Ladygin, V G ^a   Boutanaev, A M ^a  

^a INSTITUTE OF BASIC BIOLOGICAL PROBLEMS   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; ESCHERICHIA COLI;

EID: 0346355321     PISSN: 10227954     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1020279429009     Document Type: Article

References (40)

1. Hasnain, S.E., Manavathu, E.K., and Leung, W.C., DNA-Mediated Transformation of Chlamydomonas reinhardtii Cells: Use of Aminoglycoside 3′-Phosphotransferase as a Selectable Marker, Mol. Cell. Biol., 1985, vol. 512, pp. 3647-3650.
2. Mayfield, S.P. and Kindle, K.L., Stable Nuclear Transformation of Chlamydomonas reinhardtii by Using a Chlamydomonas reinhardtii Gene as the Selectable Marker, Proc. Natl. Acad. Sci. USA, 1990, vol. 87, pp. 2087-2091.
3. Hall, L.M., Taylor, K.B., and Jones, D.D., Expression of a Foreign Gene in Chlamydomonas reinhardtii, Gene, 1993, vol. 124, pp. 75-81.
4. Rochaix, J.D. and van Dillewijn, J., Transformation of the Green Alga Chlamydomonas reinhardtii with Yeast DNA, Nature, 1982, vol. 296, pp. 70-72.
5. Klein, T.M., Wolf, E.D., Wu, R., and Sanford, A.C., High-Velocity Microprojectiles for Delivering Nucleic Acids into Living Cells, Nature, 1987, vol. 327, pp. 70-73.
6. Costanzo, M.C. and Thomas, D.F., Transformation of Yeast by Agitation with Glass Beads, Genetics, 1988, vol. 120, pp. 667-670.
7. Fromm, M.E., Taylor, L.P., and Walbot, V., Expression of Genes Transferred into Monocot and Dicot Plant Cells by Electroporation, Proc. Natl. Acad. Sci. USA, 1985, vol. 82, pp. 5824-5828.
8. Boynton, I.E., Gillham, N.W., Harris, E.H., et al., Chloroplast Transformation in Chlamydomonas with High-Velocity Microprojectiles, Science, 1988, vol. 240, pp. 1534-1538.
9. Kindle, K.L., High-Frequency Nuclear Transformation in Chlamydomonas reinhardtii, Proc. Natl. Acad. Sci. USA, 1990, vol. 87, pp. 1228-1232.
10. Kindle, K.L., Richards, K.L., and Stern, D.B., Engineering Chloroplast Genome - Techniques and Capabilities for Chloroplast Transformation in Chlamydomonas reinhardtii, Proc. Natl. Acad. Sci. USA, 1991, vol. 885, pp. 1721-1725.
11. Salvador, M.L., Klein, U., and Bogorad, L., 5′ Sequences Are Important Positive and Negative Determinants of the Longevity of Chlamydomonas Chloroplast Gene Transcripts, Proc. Natl. Acad. Sci. USA, 1993, vol. 90, pp. 1556-1560.
12. Bingham, S.E., Xu, R.H., and Webber, A.N., Transformation of Chloroplasts with the psaB Gene Encoding a Polypeptide of the Photosystem-I Reaction Center, FEBS Lett., 1991, vol. 292, pp. 137-140.
13. Harris, E.H., Burkhart, B.D., Gillham, W., and Boynton, J.E., Antibiotic Resistance Mutations in the Chloroplast 16S and 23S rRNA Genes of Chlamydomonas reinhardtii, Genetics, 1989, vol. 123, pp. 281-292.
14. Kindle, K.L., Schnell, R.A., Fernandez, E., and Lefebvre, P.A., Transformation of Chlamydomonas Using the Chlamydomonas Gene for Nitrate Reductase, J. Cell Biol., 1989, vol. 109, pp. 2589-2601.
15. Diener, D.R., Curry, A.M., Johnson, K.A., et al., Rescue of a Paralyzed-Flagella Mutant of Chlamydomonas by Transformation, Proc. Natl. Acad. Sci. USA, 1990, vol. 87, pp. 5739-5743.
16. Debuchy, R., Rochaix, J.D., and Purton, S., The Argininosuccinate Lyase Gene of Chlamydomonas reinhardtii: An Important Tool for Nuclear Transformation and for Correlating the Genetic and Molecular Maps of the arg7 Locus, EMBO J., 1989, vol. 8, pp. 2803-2809.
17. Sakamoto, W., Kindle, K.L., and Stern, D.B., In Vivo Analysis of Chlamydomonas Chloroplast petD Gene Expression Using Stable Transformation of β-Glucuronidase Translation Fusions, Proc. Natl. Acad. Sci. USA, 1993, vol. 90, pp. 497-501.
18. Goldschmidt-Clermont, M., Transgenic Expression of Aminoglycoside Adenine Transferase in the Chloroplast: A Selectable Marker for Site-Directed Transformation of Chlamydomonas, Nucleic Acids Res., 1991, vol. 19, pp. 4083-4089.
19. Davies, D.R. and Plaskitt, A., Genetical and Structural Analysis of Cell Wall Formation in Chlamydomonas reinhardtii, Genet. Res., 1971, vol. 17, pp. 33-43.
20. Levine, R.P. and Ebersold, W.T., Genetics and Cytology of Chlamydomonas, Annu. Rev. Microbiol., 1960, vol. 14, pp. 197-216.
21. Ladygin, V.G., Pigment Mutants Induced in Chlamydomonas reinhardtii Dang. by N-Nitrosoethylurea and UV Irradiation, Genetika (Moscow), 1970, vol. 6, no. 3, pp. 42-50.
22. Gorman, C., Padmanabhan, R., and Howard, B.H., High Efficiency DNA-Mediated Transformation of Primate Cells, Science, 1983, vol. 221, pp. 551-553.
23. Gritz, L. and Davies, J., Plasmid-Encoded Hygromycin B Resistance: The Sequence of Hygromycin B Phosphotransferase Gene and Its Expression in Escherichia coli and Saccharomyces cerevisiae, Gene, 1983, vol. 25, pp. 179-188.
24. Maniatis, T., Fritsch, E.F., and Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1982.
25. Roffei, R.A., Golbeck, J.H., Hille, C.R., and Sayre, R.T., Photosynthetic Electron Transport in Genetically Altered Photosystem II Reaction Centers of Chloroplasts, Proc. Natl. Acad. Sci. USA, 1991, vol. 88, pp. 9122-9126.
26. Draper, J., Skott, R., and Dewry, G., Transformation of Plant Cells by DNA Transfection, Plant Genetic Transformation and Gene Expression: A Laboratory Manual, Draper, J., Scott, R., Armitage, P., and Walden, R., Eds., Oxford: Blackwell, 1988. Translated under the title Gennaya inzheneriya rastenii, Moscow: Mir, 1991, pp. 194- 232.
27. Draper, J., Skott, R., and Dewry, G., Transformation of Plant Cells by DNA Transfection, Plant Genetic Transformation and Gene Expression: A Laboratory Manual, Draper, J., Scott, R., Armitage, P., and Walden, R., Eds., Oxford: Blackwell, 1988. Translated under the title Gennaya inzheneriya rastenii, Moscow: Mir, 1991, pp. 194-232.
28. Butanaev, A.M. and Ladygin, V.G., Transformation of Chlamydomonas reinhardtii by Electroporation, Soviet-Indian Symp. on Regulation of Photosynthesis, Proskuryakov, I., Ed., Pushchino, 1990, pp. 8-9.
29. Butanaev, A.M., Efficient Transformation of Unicellular Green Alga Chlamydomonas reinhardtii by Electroporation of the Biomembrane, Biotechnologiya, 1994, no. 4, pp. 5-7.
30. Fraley, R.T., Rogers, S.G., Horsch, R.B., et al., Expression of Bacterial Genes in Plant Cells, Proc. Natl. Acad. Sci. USA, 1983, vol. 80, pp. 4803-4807.
31. Tyurin, M.V. and Livshits, V.A., Electrotransformation of Bacteria: Problems and Prospects, Usp. Sovrem. Biol., 1993, vol. 113, no. 6, pp. 659-674.
32. Brown, L.E., Sprecher, S.L., and Keller, L.R., Introduction of Exogenous DNA into Chlamydomonas reinhardtii by Electroporation, Mol. Cell. Biol., 1991, vol. 11, pp. 2328-2332.
33. De Hostos, E.L., Schilling, J., and Grossman, A.R., Structure and Expression of the Gene Encoding the Periplasmic Arylsulfatase of Chlamydomonas reinhardtii, Mol. Gen. Genet., 1989, vol. 218, pp. 229-239.
34. Rochaix, J.-D., van Dillewijn, J., and Rahire, M., Construction and Characterization of Autonomously Replicating Plasmids in the Green Unicellular Alga Chlamydomonas reinhardtii, Cell (Cambridge, Mass.), 1984, vol. 36, pp. 925-931.
35. Loppes, R., Dumont, F., Peers, B., and Piette, J., Characterization of New DNA Sequences of Chlamydomonas reinhardtii That Replicate Autonomously in Saccharomyces cerevisiae, Plant Sci. (Limerick, Irel.), 1989, vol. 59, pp. 77-86.
36. Blowers, A.D., Bogorad, L., Shark, K.B., and Sanford, J.C., Studies on Chlamydomonas Chloroplast Transformation: Foreign DNA Can Be Stably Maintained in the Chromosome, Plant Cell, 1989, vol. 1, pp. 123-132.
37. Blowers, A.D., Ellmore, G.S., Klein, U., and Bogorad, L., Transcriptional Analysis of Endogenous and Foreign Genes in Chloroplast Transformants of Chlamydomonas, Plant Cell, 1990, vol. 211, pp. 1059-1070.
38. Goldberg, A.L. and St. John, A.C., Intracellular Protein Degradation in Mammalian and Bacterial Cells: Part 2, Annu. Rev. Biochem., 1976, vol. 45, pp. 747-756.
39. Suzuki, K., Ichikawa, K., and Jigami, Y., Yeast Mutants with Enhanced Ability to Secret Human Lysozyme: Isolation and Identification of a Protease-Deficient Mutant, Mol. Gen. Genet., 1989, vol. 219, pp. 58-64.
40. Singh, M., Boutanaev, A.M., Zucchi, P., and Bogorad, L., Gene Elements That Affect the Longevity of rbcL Sequence-Containing Transcripts in Chlamydomonas reinhardtii Chloroplasts, Proc. Natl. Acad. Sci. USA, 2001, vol. 98, pp. 2289-2294.