Domain switching between hrpR and hrpS affects the regulatory function of the hybrid genes in Pseudomonas syringae pv. phaseolicola

Molecular Plant Pathology

Volumn 1, Issue 4, 2000, Pages 233-241

  Schuster, Martina ^a   Grimm, Charlotte ^a  

^a UNIVERSITY OF VIENNA   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0346312221     PISSN: 14646722     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1364-3703.2000.00028.x     Document Type: Article

References (42)

1. Alfano, J.R. and Collmer, A. (1997) The type III (Hrp) secretion pathway of plant pathogenic bacteria: trafficking harpins, Avr proteins, and death. J. Bacteriol. 179, 5655-5662.
2. Ausubel, F.M. (1989) Current Protocols in Molecular Biology. New York: Green Publishing Associates/Wiley Interscience.
3. Berger, D., Narberhaus, F., Lee, H.S. and Kustu, S. (1995) In vitro studies of the domains of the nitrogen fixation regulatory protein NifA. J. Bacteriol. 177, 191-199.
4. Birnboim, H.C. and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmids. Nucl. Acids Res. 7, 1513-1523.
5. Buck, M. and Cannon, W. (1989) Mutations in the RNA polymerase recognition sequence of the Klebsiella pneumoniae nifH promoter permitting transcriptional activation in the absence of NifA binding to upstream activator sequences. Nucl. Acids Res. 17, 2597-2612.
6. Buck, M., Woodcock, J., Cannon, W., Mitchenall, LA. and Drummond, M. (1987) Positional requirements for the function of nif-specific upstream activator sequences. Mol. Gen. Genet. 210, 140-144.
7. Drummond, M.H., Contreras, A. and Mitchenall, L.A. (1990) The function of isolated domains and chimeric proteins constructed from the transcriptional activators NifA and NtrC of Klebsiella pneumoniae. Mol. Microbiol. 4, 29-37.
8. Feinberg, A.P. and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6-13.
9. Fellay, R., Rahme, L.G., Mindrinos, R.D., Frederick, R.D., Pisi, A. and Panopoulos, N.J. (1989) Genes and signals controlling the Pseudomonas syringae pv. phaseolicola-plant interaction. In Advances in Molecular Genetics of Plant-Microbe Interactions, Vol. 1 (Hennecke, H. and Verma, D.P.S., eds). Dordrecht, The Netherlands: Kluwer Academic Publishers.
10. Genin, S., Gough, C.L, Zischek, C. and Boucher, C.A. (1992) Evidence that the hrpB gene encodes a positive regulator of pathogenicity genes from Pseudomonas solanacearum. Mol. Microbiol. 6, 3065-3076.
11. Grimm, C. and Panopoulos, N.J. (1989) The predicted protein product of a pathogenicity locus from Pseudomonas syringae pv. phaseolicola is homologous to a highly conserved domain of several prokaryotic regulatory proteins. J. Bacteriol. 171, 5031-5038.
12. Grimm, C., Aufsatz, W. and Panopoulos, N.J. (1995) The hrpRS locus of Pseudomonas syringae pv. phaseolicola constitutes a complex regulatory unit. Mol. Microbiol. 15, 155-165.
13. Harrison, S.C. and Aggarwal, A.K. (1990) DNA recognition by proteins with the helix-turn-helix motif. Annu. Rev. Biochem. 59, 933-969.
14. Huala, E. and Ausubel, F.M. (1989) The central domain of Rhizobium meliloti NifA is sufficient to activate transcription from the R. meliloti nifH promoter. J. Bacteriol. 171, 3354-3365.
15. Huala, E., Stigter, J. and Ausubel, F.M. (1992) The central domain of Rhizobium leguminosarum DctD functions independently to activate transcription. J. Bacteriol. 174, 1428-1431.
16. Innes, R.W., Bent, A.F., Kunkel, B.N., Bisgrove, S.R. and Staskawicz, B.J. (1993) Molecular analysis of avirulence gene avrRpt2 and identification of a putative regulatory sequence common to all known Pseudomonas syringae avirulence genes. J. Bacteriol. 175, 4859-4869.
17. Kim, J.F., Wei, Z.-M. and Beer, S.V. (1997) The hrpA and hrpC operons of Erwinia amylovora encode components of a type III pathway that secretes harpin. J. Bacteriol. 179, 1690-1697.
18. King, E.O., Wood, M.K. and Raney, D.E. (1954) Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44, 301-307.
19. 54-dependent dctA promoters. J. Bacteriol. 172, 3888-3897.
20. 54-dependent transcriptional activator. J. Biol. Chem. 269, 20 401-20 409.
21. Lindgren, P.B., Peet, R.C. and Panopoulos, N.J. (1986) Gene cluster of Pseudomonas syringae pv. phaseolicola controls pathogenicity of bean plants and hypersensitivity on non-host plants. J. Bacteriol. 168, 512-522.
22. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
23. Mansfield, J., Jenner, C., Hockenhull, R., Bennett, M. and Stewart, R. (1994) Characterization of avrPphE, a gene for cultivar specific avirulence from Pseudomonas syringae pv. phaseolicola which is physically linked to hrpY, a new hrp gene identified in the halo-blight bacterium. Mol. Plant-Microbe Interact. 7, 726-739.
24. Melton, D.A., Krieg, P., Rebagliati, M.R., Maniatis, T., Zinn, K. and Green, M.R. (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage Sp6 promoter. Nucl. Acids Res. 12, 7035-7056.
25. 54 bacterial enhancer binding protein family: mechanism of action and phylogenetic relationship of their functional domains. J. Bacteriol. 175, 6067-6074.
26. North, A.K., Klose, K.E., Stedman, K.M. and Kustu, S. (1993) Prokaryotic enhancer-binding proteins reflect eukaryote-like modularity: the puzzle of nitrogen regulatory protein C. J. Bacteriol. 175, 4267-4273.
27. 54-dependent transcriptional activator of Pseudomonas putida. J. Mol. Biol. 258, 575-587.
28. 54-dependent activator XyIR triggers a protein multimerization cycle catalyzed by UAS DNA. Cell, 86, 331-339.
29. Salmeron, J.M. and Staskawicz, B.J. (1993) Molecular characterization and hip dependence of the avirulence gene avrPto from Pseudomonas syringae pv. tomato. Mol. Gen. Genet. 239, 6-16.
30. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
31. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5467.
32. Spaink, H.P., Okker, R.J.H., Wijffelman, C.A., Pees, E. and Lugtenberg, B.J.J. (1987) Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Plant Mol. Biol. 9, 27-39.
33. Staskawicz, B.J., Dahlbeck, D., Keen, N.T. and Napoli, C (1987) Molecular characterization of cloned avirulence genes from Race 0 and Race 1 of Pseudomonas syringae pv. glycinea. J. Bacteriol. 169, 5789-5794.
34. Van Gijsegem, F., Genin, S. and Boucher, C. (1994) hrp and avr genes, key determinants controlling the interactions between plants and Gram-negative phytopathogenic bacteria. In Pathogenesis and Host-Parasite Specificity in Plant Diseases: Histopathological, Biochemical, Genetic and Molecular Basis, Vol. 1 (Shingh, U.S., Kohomoto, K. and Shingh, R.P., eds), pp. 273-292. Oxford: Pergamon Press.
35. Vieira, J. and Messing, J. (1982) The pUC plasmids and M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene, 19, 259-268.
36. Vivian, A., Gibbon, M.J. and Murillo, J. (1997) The molecular genetics of specificity determinants in plant pathogenic bacteria. In The Gene-for-Gene Relationship in Host-Parasite Interactions (Crute, I.R., Burden, J.J. and Holub, E.B., eds), pp. 293-328. Wallingford, UK: C.A.B. International.
37. Weiss, D., Batut, J., Klose, K.E., Keener, J. and Kustu, S. (1991) The phosphorylated form of the enhancer binding protein NtrC has an ATPase activity that is essential for activation of transcription. Cell, 67, 155-167.
38. Wengelnik, K., Van den Ackerveken, G. and Bonas, U. (1996) HrpG, a key hrp regulatory protein of Xanthomonas campestris pv. vesicatoria is homologous to two-component response regulators. Mol. Plant-Microbe Interact. 9, 704-712.
39. Willis, D.K., Rich, J.J. and Hrabak, E. (1991) hrp genes of phytopathogenic bacteria. Mol. Plant-Microbe Interact. 4, 132-138.
40. Wyman, C., Rombel, I., North, A.K., Bustamante, C. and Kustu, S. (1997) Unusual oligomerization required for activity of NtrC, a bacterial enhancer-binding protein. Science, 275, 1658-1661.
41. Xiao, Y., Heu, S., Yi, J., Lu, Y. and Hutcheson, S.W. (1994) Identification of a putative alternate sigma factor and characterization of a multicomponent regulatory cascade controlling the expression of Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genes. J. Bacteriol. 176, 1025-1036.
42. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33, 103-119.