A Simple and Sensitive Polymerase Chain Reaction Method for the Detection of Food-Related Bacteria

Food Science and Technology Research

Volumn 6, Issue 2, 2000, Pages 115-118

  Nagashima, Koji ^a   Shimizu, Takeshi ^a   Takeshi, Kouichi ^b   Kawakami, Makoto ^a   Yasokawa, Daisuke ^a   Nakagawa, Ryoji ^a   Okumura, Yukihiro ^a  

^a Hokkaido Food Processing Research Center   (Japan)

^b HOKKAIDO INSTITUTE OF PUBLIC HEALTH   (Japan)

Author keywords

Achromopeptidase; Chelex 100; DNA preparation; Food; PCR

Indexed keywords

EID: 0346300408     PISSN: 13446606     EISSN: None     Source Type: Journal    
DOI: 10.3136/fstr.6.115     Document Type: Article

References (12)

1. Barsotti, O., Renaud, F., Freney, J., Benay, G., Decoret, D. and Dumont, J. (1987). Rapid isolation of DNA from Actinomyces. Ann. Inst. Pasteur Microbiol. 138, 529-536.
2. Bej, A.K., McCarty, S.C. and Atlas, R.M. (1991). Detection of coli-form bacteria and Esherichia coli by muliplex polymerase chain reaction: Comparison with defined substrate and plating methods for water quality monitoring. Appl. Environ. Microbiol., 57, 2429-2432.
3. Brakstad, O.G., Aasbakk, K. and Maeland, J.A. (1992). Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J. Clin. Microbol., 30, 1654-1660.
4. Edwards, U., Rogall, T. Blöcker, H., Emde, M. and Böttger, C. (1989). Isolation of direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res., 17, 7843-7853.
5. Ezaki, T. and Suzuki, S. (1982). Achromopeptidase for lysis of anaerobic gram-positive cocci. J. Clin. Microbiol., 16, 844-846.
6. Lee, C., Pan, S. and Chen, C. (1995). Sequence of a cloned pR72H fragment and its use for detection of Vibrio parahaemolyticus in shellfish with the PCR. Appl. Environ. Microbiol., 61, 1311-1317.
7. Leonard, R.B. and Carroll, K.C. (1997). Rapid lysis of gram-positive cocci for pulsed-field gel electrophoresis using achromopeptidase. Diagn. Mol. Pathol., 6, 288-291.
8. Li, S., Norioka, S. and Sakiyama, F. (1998). Bacteriolytic activity and specificity of Achromobacter beta-lytic protease. J. Biochem., 124, 332-339.
9. Neefs, J. M., Van de Peer, Y., Hendriks, L. and de Wachter, R. (1990). Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res., 18(Suppl), 2237-2317.
10. Schraft, H. and Griffiths, M.W. (1995). Specific oligonucleotide primers for detection of lecitithinase-positive Bacillus spp. by PCR. Appl. Environ. Microbiol., 61, 98-102.
11. Walsh, P.S., Metzger, D.A. and Higuchi, R. (1991). Chelex® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques, 10, 506-513.
12. Yazdankhah, S.P., Hellemann, A.L., Ronningen, K. and Olsen, E. (1998). Rapid and sensitive detection of Staphylococcus species in milk by ELISA based on monodispersed magnetic particles. Vet. Microbiol., 62, 17-26.