In vitro high level protein S expression after modification of protein S cDNA

Thrombosis and Haemostasis

Volumn 90, Issue 6, 2003, Pages 1214-1215

  Rezende, Suely M ^a   Razzari, Cristina ^b   Simmonds, Rachel E ^b  

^a FUNDAÇÃO HEMOMINAS   (Brazil)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; PROTEIN S;

CODON; CONCENTRATION (PARAMETERS); DNA MODIFICATION; DNA SEQUENCE; GENE AMPLIFICATION; GENETIC TRANSFECTION; HEMOSTASIS; HUMAN; IN VITRO STUDY; LETTER; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION;

EID: 0346272820     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-0037-1613426     Document Type: Letter

References (7)

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3. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 1986; 44: 283-92.
4. Kaufman RJ. Vectors used for expression in mammalian cells. Methods in Enzymology 1990; 185: 487-511.
5. Rezende SM, Lane DA, Zoller B, et al. Genetic and phenotypic variability between families with hereditary protein S deficiency. Thromb Haemost 2002; 87: 258-65.
6. Rezende SM, Lane DA, Mille-Baker B, et al. Protein S Gla-domain mutations causing impaired Ca2+-induced phospholipid binding and severe functional deficiency. Blood 2002; 100: 2812-9.
7. Stenberg Y, Drakenberg T, Dahlback B, et al. Characterization of recombinant epidermal growth factor (EGF)-like modules from vitamin-K-dependent protein S expressed in Spodoptera cells - The cofactor activity depends on the N-terminal EGF module in human protein. Eur J Biochem 1998; 251: 558-64.