Clustered-charge to alanine scanning mutagenesis of the Mal63 MAL-activator C-terminal regulatory domain

Current Genetics

Volumn 44, Issue 4, 2003, Pages 173-183

  Danzi, Sara E ^a,b   Bali, Mehtap ^a   Michels, Corinne A ^a  

^a CITY UNIVERSITY OF NEW YORK   (United States)

^b North Shore LIJ Research Institute ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ALPHA GLUCOSIDASE; MAL63 PROTEIN; MALTOSE; PERMEASE; PROTEIN; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; GENE CLUSTER; GENE CONSTRUCT; GENE EXPRESSION; GENE IDENTIFICATION; GENE MAPPING; GENE MUTATION; GENETIC CODE; GENETIC REGULATION; GENETIC TRANSCRIPTION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; STRUCTURAL GENE; TRANSACTIVATION; YEAST;

ALPHA-GLUCOSIDASES; AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CARBON RADIOISOTOPES; DNA PRIMERS; ENZYME INDUCTION; MALTOSE; MEMBRANE TRANSPORT PROTEINS; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; MUTAGENESIS; MUTATION; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS;

SACCHAROMYCES CEREVISIAE;

EID: 0346252597     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-003-0429-9     Document Type: Article

References (27)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (2002) Current Protocols in Molecular Biology. Wiley, New York
2. Bohen SP, Yamamoto KR (1993) Isolation of Hsp90 mutants by screening for decreased steroid receptor function. Proc Natl Acad Sci USA 90:11424-11428
3. Cheng Q, Michels CA (1991) MAL11 and MAL61 encode the inducible high-affinity maltose transporter of Saccharomyces cerevisiae. J Bacteriol 173:1817-1820
4. Danzi SE, Zhang B, Michels CD (2000) Alterations in the Saccharomyces MAL-activator cause constitutivity but can be suppressed by intragenic mutations. Curr Genet 294:233-240
5. Des Etages SA, Saxena D, Huang HL, Falvey DA, Barber D, Brandriss MC (2001) Conformational changes play a role in regulating the activity of the proline utilization pathway-specific regulator in Saccharomyces cerevisiae. Mol Microbiol 40:890-899
6. Dubin RA, Needleman RB, Gossett D, Michels CA (1985) Identification of the structural gene encoding maltase within the MAL6 locus of Saccharomyces cerevisiae. J Bacteriol 164:605-610
7. Dubin RA, Charron MJ, Haut SR, Needleman RB, Michels CA (1988) Constitutive expression of the maltose fermentative enzymes in Saccharomyces carlsbergenesis is dependent upon the mutational activation of a non-essential homolog of MAL63. Mol Cell Biol 8:1027-1035
8. El Alami M, Feller A, Pierard A, Dubois E (2002) The proper folding of a long C-terminal segment of the yeast Lys14p regulator is required for activation of LYS genes in response to the metabolic effector. Mol Microbiol 43:1629-1639
9. Elledge SJ, Davis RW (1988) A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70:303-312
10. Gibson AW, Wojciechowicz LA, Danzi SE, Zhang B, Kim JH, Hu Z, Michels CA (1997) Constitutive mutations of the Saccharomyces cerevisiae MAL-activator genes MAL23, MAL43, MAL63, and mal64. Genetics 146:1287-1298
11. Hach A, Hon T, Zhang L (1999) A new class of repression modules is critical for heme regulation of the yeast transcriptional activator Hap1. Mol Cell Biol 19:4324-4333
12. Higgins VJ, Braidwood M, Bissinger P, Dawes IW, Attfield PV (1999) Leu343Phe substitution in the Malz3 protein of Saccharomyces cerevisiae increases the constitutivity and glucose insensitivity of MAL gene expression. Curr Genet 35:491-498
13. Hu Z, Nehlin JO, Ronne H, Michels CA (1995) MIG1-dependent and MIG1-independent glucose regulation of MAL gene expression in Saccharomyces cerevisiae. Gurr Genet 28:258-266
14. Hu Z, Gibson AW, Kim JH, Wojciechowicz LA, Zhang B, Michels CA (1999) Functional domain analysis of the Saccharomyces MAL-activator. Curr Genet 36:1-12
15. Kelly R, Kwon-Chung KJ (1992) A zinc finger protein from Candida albicans is involved in sucrose utilization. J Bacteriol 174:222-232
16. Kim J, Michels CA (1988) The MAL63 gene of Saccharomyces encodes a cysteine-zinc finger protein. Curr Genet 14:319-323
17. Lee HC, Hon T, Zhang L (2002) The molecular chaperone Hsp90 mediates heme activation of the yeast transcriptional activator Hap1. J Biol Chem 277:7430-7437
18. Leuther K, Salmeron J, Johnston S (1993) Genetic evidence that an activation domain of GAL4 does not require acidity and may form a β sheet. Cell 72:575-585
19. Medintz I, Jiang H, Han EK, Cui W, Michels CA (1996) Characterization of the glucose-induced inactivation of maltose permease in Saccharomyces cerevisiae. J Bacteriol 178:2245-2254
20. Needleman RB (1991) Control of maltase synthesis in yeast. Mol Microbiol 5:2070-2084
21. Peng G, Hopper JE (2000) Evidence for Gal3p's cytoplasmic location and Gal80p's dual cytoplasmic-nuclear location implicates new mechanisms for controlling Gal4p activity in Saccharomyces cerevisiae. Mol Cell Biol 20:5140-5148
22. Pratt WB (1998) The Hsp90-based chaperone system: Involvement in signal transduction from a variety of hormone and growth factor receptors. Proc Soc Exp Biol Med 217:420-434
23. Pratt WB, Toft DO (2003) Regulation of signaling protein function and trafficking by the hsp90/hsp70-based chaperone machinery. Proc Soc Exp Biol Med 228:111-133
24. Sikorsky RS, Heiter P (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27
25. Sollitti P, Marmur J (1988) Primary structure of the regulatory gene from the MAL6 locus of Saccharomyces carlsbergensis. Mol Gen Genet 27: 509-516
26. Wang X, Bali M, Medintz I, Michels CA (2002) Intracellular maltose is sufficient to induce MAL gene expression in Saccharomyces cerevisiae. Eukaryot Cell 1:696-703
27. Wertman KF, Drubin DG, Botstein D (1992) Systematic mutational analysis of the yeast ACT1 gene. Genetics 132: 337-350