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Volumn 5, Issue 1, 2000, Pages 13-16

Simple purification of Escherichia coli-derived recombinant human interleukin-2 expressed with N-terminus fusion of glucagon

Author keywords

Glucagon; Human interleukin 2; N terminus fusion; Purification process

Indexed keywords


EID: 0346225771     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02932346     Document Type: Article
Times cited : (2)

References (11)
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  • 2
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  • 3
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    • Lin, S. J., R. L. Roberts, B. J. Ank, Q. H. Nguyen, E. K. Thomas, and E. R. Stiehm (1997) Human immunodeficiency virus (HIV) type-1 GP120-specific cell-mediated cytotoxicity (CMC) and natural killer (NK) activity in HIV-infected (HIV+) subjects: enhancement with interleukin-2 (IL-2), IL-12, and IL-15. Clin. Immunol. Immunopathol. 82: 163-173.
    • (1997) Clin. Immunol. Immunopathol. , vol.82 , pp. 163-173
    • Lin, S.J.1    Roberts, R.L.2    Ank, B.J.3    Nguyen, Q.H.4    Thomas, E.K.5    Stiehm, E.R.6
  • 5
    • 0034238093 scopus 로고    scopus 로고
    • Glucagon-induced self-association of recombinant proteins in Escherichia coli and affinity purification using a fragment of glucagon receptor
    • Manuscript submitted to
    • Kim, D.-Y., J. Lee, V. Saraswat, and Y.-H. Park, Glucagon-induced self-association of recombinant proteins in Escherichia coli and affinity purification using a fragment of glucagon receptor. Manuscript submitted to Biotechnol. Bioeng.
    • Biotechnol. Bioeng.
    • Kim, D.-Y.1    Lee, J.2    Saraswat, V.3    Park, Y.-H.4
  • 6
    • 0023222405 scopus 로고
    • Purification and renaturation of recombinant human interleukin-2
    • Weir, M. P. and J. Sparks (1987) Purification and renaturation of recombinant human interleukin-2. Biochem. J. 245: 85-91.
    • (1987) Biochem. J. , vol.245 , pp. 85-91
    • Weir, M.P.1    Sparks, J.2
  • 8
    • 52849121244 scopus 로고    scopus 로고
    • (1999) Preparation of peptides by use of glucagon and its variants as a fusion expression partner. Korea Patent Application No. 99-7641
    • Park, Y.-H., J. Lee, and D.-Y. Kim (1999) Preparation of peptides by use of glucagon and its variants as a fusion expression partner. Korea Patent Application No. 99-7641.
    • Park, Y.-H.1    Lee, J.2    Kim, D.-Y.3
  • 9
    • 0032824865 scopus 로고    scopus 로고
    • Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level
    • Saraswat, V., D.-Y. Kim, J. Lee, and Y.-H. Park (1999) Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level. FEMS Microbiol. Lett. 179: 367-373.
    • (1999) FEMS Microbiol. Lett. , vol.179 , pp. 367-373
    • Saraswat, V.1    Kim, D.-Y.2    Lee, J.3    Park, Y.-H.4
  • 10
    • 0023198037 scopus 로고
    • Characterization of disulfide bonds in recombinant proteins: Reduced human interleukin 2 in inclusion bodies and its oxidative refolding
    • Tsuji, T., R. Nakagawa, N. Sugimoto, and K. Fukuhara (1987) Characterization of disulfide bonds in recombinant proteins: reduced human interleukin 2 in inclusion bodies and its oxidative refolding. Biochemistry 26: 129-34.
    • (1987) Biochemistry , vol.26 , pp. 129-134
    • Tsuji, T.1    Nakagawa, R.2    Sugimoto, N.3    Fukuhara, K.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.