Enzymic, spectroscopic and calorimetric studies of a recombinant dextranase expressed in Pichia pastoris

Biotechnology and Applied Biochemistry

Volumn 38, Issue 3, 2003, Pages 211-221

  Beldarraín, Alejandro ^a   Acosta, Niuris ^b   Betancourt, Lázaro ^a   González, Luis J ^a   Pons, Tirso ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

^b UNIVERSITY OF HAVANA   (Cuba)

Author keywords

Circular dichroism (CD); Dextranase; Differential scanning calorimetry; Fluorescence; Mass spectrometry (MS); Unfolding

Indexed keywords

AGGLOMERATION; BACTERIA; CONFORMATIONS; DESORPTION; DIFFERENTIAL SCANNING CALORIMETRY; FLUORESCENCE; FUNGI; IONIZATION; PH EFFECTS; SPECTROSCOPIC ANALYSIS; THERMAL EFFECTS;

DENATURATION PROCESS;

ENZYMES;

DEXTRANASE; GLYCOSIDASE; RECOMBINANT ENZYME;

ACIDITY; ARTICLE; BETA SHEET; CIRCULAR DICHROISM; DIFFERENTIAL SCANNING CALORIMETRY; DISULFIDE BOND; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME CONFORMATION; ENZYME DENATURATION; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; FLUORESCENCE; GENE EXPRESSION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MULTIGENE FAMILY; NONHUMAN; PH; PICHIA PASTORIS; PROTEIN AGGREGATION; PROTEIN SECONDARY STRUCTURE; TEMPERATURE DEPENDENCE;

AMINO ACID SEQUENCE; CALORIMETRY, DIFFERENTIAL SCANNING; DEXTRANASE; ENZYME ACTIVATION; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; PENICILLIUM; PICHIA; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE;

BACTERIA (MICROORGANISMS); FUNGI; PICHIA PASTORIS;

EID: 0346216957     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20030063     Document Type: Article

References (38)

1. Inmrie, F. K. and Tulbury, R. H. (1972) Sugar Technol. Rev. I. 291-361
2. Fulcher, R. P. and Inkerman, P. A. (1976) Proc. Queensl. Soc. Sugar Cane Technol. 43rd Conf., Queensland, Australia, 295-305
3. Anchorman, P. A. (1980) Proc. Int. Soc. Sugar Cane Technol. 17, 2411-2427
4. Walker, G. J. (1978) Int. Rev. Biochem. 16, 75-126
5. Roca, H., García, B., Rodríguez, E., Mateu, D., Coroas, L., Cremata, J., García, R., Pons, T. and Delgado, J. (1996) Yeast 12, 1187-1200
6. Betancourt, L., García, R., González, J., Montesinos, R., Quintero, Q., Takao, T., Shimonishi, Y. and Cremata, J. (2001) FEMS Yeast Res. 1, 151-160
7. Larsson, A. M., Ståhlberg, J. and Jones, T. A. (2002) Acta Crystallogr. D 58, 346-348
8. International Conference on Harmonisation (1999) ICH Topic Q6B Specifications: test procedures and acceptance criteria for biotechnological/ biological products, Geneva, Switzerland
9. Wang, W. (1999) Int. J. Pharm. 185, 129-188
10. Pace, N. C., Vajdos, F. L., Grimsley, G. and Gray, V. (1995) Protein Sci. 4, 2411-2423
11. Miller, G. L. (1959) Anal. Chem. 31, 426-428
12. Kosaric, N., Yu, K. and Zajic, J. E. (1973) Biotechnol. Bioeng. 71, 729-74
13. Chaplin, M. F. and Kennedy, J. F. (1986) in Carbohydrate Analysis: a Practical Approach (Chaplin, M. F. and Kennedy, J. F., eds.), chapter 2.2.2, pp. 3-5, OIRL Press, Oxford
14. Perczel, A., Park, K. and Fasman, G. D. ( 1992) Anal. Biochem. 203, 38-53
15. Cortijo, M., Beldarraín, A., Molina, A. and Lopez-Lacomba, J. (1995) Meas. Sci. Technol. 6, 1086-1092
16. Závodszky, P., Kardos, J., Svingor, A. and Petsko, G. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 7406-7411
17. Wynter, C., Patel, B. K. C., Bain, P., de Jersey, J., Hamilton, S. and Inkerman, P. A. (1996) FEMS Microbiol. Lett. 140, 271-276
18. Raíces, M., Moleiro, M. C., Li, I., Morera, V., Roca, H., Delgado, J. and Cremata, J. (1991) Biotecnol. Aplicada 8, 248-255
19. Okazaki, K., Abe, T., Saruwatari, K., Kato, F., Maruyama, K. and Tagawa, K. (1992) Biosci. Biotechvol. Biochem. 56, 1401-1405
20. Soo-Young, W. and Curtiss, III, R. (1994) J. Bacteriol. 176, 3839-3850
21. Okazaki, K., Yong-Jin, S., Sawamura, Y. and Hayakawa, S. (1995) J. Ferment. Bioeng. 79, 370-372.
22. García, F., Di Pietro, A. and Roncero, I. (1997) FEMS Microbiol. Lett. 154, 37-43
23. Arnold, W. N., Tan Binh, P. N. and Mann, L. C. (1998). Arch. Microbiol. 170, 91-98
24. Pons, T., Chinea, G., Olmea, O., Beldarraín, A., Roca, H., Padrón, G. and Valencia, A. (1998) Proteins Struct. Funct. Genet. 31, 345-354
25. Sanchez-Ruíz, J. M. (1995) in Subcellular Biochemistry. Protein: Structure, Function and Engineering, vol. 24 (Biswas, B. B. and Roy, S., eds.), pp. 133-175, Plenum Press, New York
26. Privalov, P. (1979) Adv. Protein Chem. 33, 167-241
27. Sanchez-Ruiz, J. M. (1992) Biophys. J. 61, 921-935
28. Marcos, M. J., Chechín, R., Arrondo, J. L., Zhadan, G. G., Villar, E. and Shnyrov, V. L. (1999) FEBS Lett. 443, 192-196
29. Marcos, M. J., Villar, E., Gavilanes, F., Zhadan, G. G. and Shnyrov, V. L. (2000) Eur. J. Biochem. 267, 2127-2132
30. Rochu, D., Georges, C., Répiton, J., Viguié, N., Saliou, B., Bon, C. and Masson, P. (2001) Biochim. Biophys. Acta 1545, 216-226
31. Mozhaev, V. V. (1993) Trends Biotechnol. 11, 88-95
32. Dill, K. A. (1990) Biochemistry 29, 7133-7155
33. Fariselli, P., Riccobelli, P. and Casadio, R. (1999) Proteins 36, 340-346
34. Henrissat, B. (1991) Biochem. J. 280, 309-316
35. Faulstich, H., Tews, P. and Heintz, D. (1993) Anal. Biochem. 208, 357-362
36. Asahi, M., Fujii, T., Takao, T., Kuzuya, T., Hori, M., Shimonishi, Y. and Taniguchi, N. J. (1997) J. Biol. Chem. 272, 19152-19157
37. Betancourt, L., Takao, T., Hernández, L., Padron, G. and Shimonishi, Y. (1999) J. Mass Spectrom. 34, 169-174
38. Pace, N., Shirley, B. A., McNutt, M., and Gajiwala, K. (1996) FASEB J. 10, 75-83