A New Purification Method of G-actin Extracted from the Acetone-Dried Myofibril-Powder Using Ammonium Sulfate Fractionation. A Comparison with the Conventional G-actin and High-Salt Sensitivity

Fisheries Science

Volumn 63, Issue 3, 1997, Pages 397-402

  Torigai, Makoto ^a   Konno, Kunihiko ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Actin; Ammonium sulfate fractionation; Carp myofibril; G actin purification; High salt treatment; Myosin interaction

Indexed keywords

CYPRINUS CARPIO;

EID: 0346158702     PISSN: 09199268     EISSN: None     Source Type: Journal    
DOI: 10.2331/fishsci.63.397     Document Type: Article

References (19)

1. W. F. H. M. Mommaerts: Reversible polymerization and ultracentrifugal purification of actin. J. Biol. Chem., 188, 559-565 (1951).
2. M. Kitao, N. Seki, and K. Arai: Extraction and polymerizing ability of crude G-actin from fish muscle. Nippon Suisan Gakkaishi, 39, 1263-1269 (1973) (in Japanese).
3. J. A. Spudich and S. Watt: Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin. J. Biol. Chem., 246, 4866-4871 (1971).
4. S. Watabe, Y. Itoh, and K. Hashimoto: Isolation and characterization of actin from mackerel dark muscle. Nippon Suisan Gakkaishi, 49, 491-497 (1983).
5. K. Nishita and O. Otani: Properties of actins from smooth adductor muscles of surf clam and scallop. Nippon Suisan Gakkaishi, 45, 347-351 (1979) (in Japanese).
6. Y. Yamashita, K. Arai, and K. Nishita: Thermal stabilities of synthetic actomyosin in various combinations of myosin and actin from fish, scallop and rabbit muscle. Nippon Suisan Gakkaishi, 44, 485-289 (1978) (in Japanese).
7. K. Konno: Two calcium regulatory systems in squid muscle. J. Biochem., 84, 1431-1441 (1978).
8. A. Wakameda and K. Arai: Dissociation of carp myosin B into actin and myosin in the presence of a high concentration of NaCl. Nippon Suisan Gakkaishi, 52, 293-300 (1986) (in Japanese).
9. A. Wakameda and K. Arai: The amount of actin dissociated from myosin B in the presence of NaCl. Nippon Suisan Gakkaishi, 51, 497-502 (1985) (in Japanese).
10. N. Katoh, H. Uchiyama, S. Tsukamoto, and K. Arai: A biochemical study of fish myofibrillar ATPase. Nippon Suisan Gakkaishi, 43, 857-867 (1977) (in Japanese).
11. H. Koseki, S. Kato, and K. Konno: Myosin extractability as a sensilive probe for the thermal denaturation of carp myofibrils. Nippon Suisan Gakkaishi, 59, 515-518 (1993) (in Japanese).
12. K. Konno, S. Kato, and M. Eko: Rapid method for isolation of myosin subfragment-1 from carp myofibrils. Nippon Suisan Gakkaishi, 56, 1885-1890 (1990) (in Japanese).
13. U.K. Laemmli : Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
14. M. Barany and K. Barany: Preparation of actin without extraction of myosin. Nature, 179, 818 (1957).
15. K. Konno and N. Seki: On the preparation of troponin from carp muscle. Nippon Suisan Gakkaishi, 41, 1327-1333 (1975) (in Japanese).
16. S. Ebashi, A. Kodama, and F. Ebashi: Troponin. I. Preparation and physiological function. J. Biochem., 64, 465-477 (1968).
17. G. R. Holmes, D.E. Goll, and A. Suzuki: Effect of α-actinin on ac- tin viscosity. Biochem. Biophys. Acta, 253, 240-253 (1971).
18. N. Seki, T. Fushimi, and T. Watanabe: Effect of temperature on fish a-actinin and F-actin interaction. Nippon Suisan Gakkaishi, 52, 267-274 (1986) (in Japanese).
19. M. Torigai and K. Konno: Pyrophosphate-accelerated actin denaturation mechanism in myofibril. Fisheries Sci., 62, 307-311 (1996).