Photometric assay for measuring the intracellular concentration of branched-chain amino acids in bacteria

Journal of Microbiological Methods

Volumn 56, Issue 1, 2004, Pages 133-136

  Petranovic, Dina ^a   Mijakovic, Ivan ^b  

^a CEMAGREF   (France)

^b CNRS   (France)

Author keywords

Branched chain amino acids; Intracellular concentration; Leucine dehydrogenase

Indexed keywords

BACTERIAL PROTEIN; BRANCHED CHAIN AMINO ACID;

ADAPTATION; ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL; BLOOD SAMPLING; CONTROLLED STUDY; ESCHERICHIA COLI; EXPERIMENTAL MODEL; EXPERIMENTAL TEST; LACTOCOCCUS LACTIS; MICROBIAL ACTIVITY; MOLECULAR BIOLOGY; NONHUMAN; PHOTOMETRY; PRIORITY JOURNAL;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LACTOCOCCUS LACTIS;

EID: 0346099179     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2003.10.002     Document Type: Article

References (12)

1. Andersen H.W., Solem C., Hammer K., Jensen P.R. Twofold reduction of phosphofructokinase activity in Lactococcus lactis results in strong decreases in growth rate and in glycolytic flux. J. Bacteriol. 183:2001;3458-3467.
2. Beckett P.R. Spectrophotometric assay for measuring branched-chain amino acids. Methods Enzymol. 324:2000;40-47.
3. Belitsky B.R., Gustafsson M.C.U., Sonenshein A.L., von Wachenfeldt C. An Lrp-like gene of Bacillus subtilis involved in branched-chain amino acid transport. J. Bacteriol. 179:1997;5448-5457.
4. Calvo J.M., Matthews R.G. The leucine-responsive regulatory protein, a global regulator of metabolism in Escherichia coli. Microbiol. Rev. 58:1994;466-490.
5. Cayley D.S., Guttman H.J., Record M.T. Jr. Biophysical characterization of changes in amounts and activity of Escherichia coli cell and compartment water and turgor pressure in response to osmotic stress. Biophys. J. 78:2000;1748-1764.
6. D'Ari R., Lin R.T., Newman E.B. The leucine-responsive regulatory protein: more than a regulator? Trends Biochem. Sci. 18:1993;260-263.
7. Freese E.B., Vasantha N., Freese E. Induction of sporulation in developmental mutants in Bacillus subtilis. Mol. Gen. Genet. 170:1979;67-74.
8. Fujita Y., Freese E. Purification and properties of Fructose-1,6- bisphosphatase of Bacillus subtilis. J. Biol. Chem. 245:1979;5340-5349.
9. Guedon E., Serror P., Ehrlich S.D., Renault P., Delorme C. Pleiotropic transcriptional repressor CodY senses the intracellular pool of branched-chain amino acids in Lactococcus lactis. Mol. Microbiol. 40:2001;1227-1239.
10. Livesey G., Lund P. Determination of branched-chain amino and keto acids with leucine dehydrogenase. Methods Enzymol. 166:1988;3-10.
11. Pardee A.B., Jacob F., Monod J. The genetic control and cytoplasmic expression of "inducibility" in the synthesis of beta-galactosidase by Escherichia coli. J. Mol. Biol. 1:1959;165-178.
12. Sissler M., Delorme C., Bond J., Ehrlich S.D., Renault P., Franklyn C. An aminoacyl-tRNA synthetase paralog with a catalytic role in histidine biosynthesis. Proc. Natl. Acad. Sci. U. S. A. 96:1999;8985-8990.