Quantification of DNA synthesis from different pathways in cultured human fibroblasts and myocytes

Metabolism: Clinical and Experimental

Volumn 53, Issue 1, 2004, Pages 128-133

  Martini, Wenjun Z ^a   Chinkes, David L ^a   Wolfe, Robert R ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON 13; DEOXYRIBONUCLEOSIDE; DNA; GLUCOSE; GLYCINE; NITROGEN 15;

ARTICLE; CELL COUNT; CELL CULTURE; CELL GROWTH; CELL PROLIFERATION; CELL TYPE; CONTROLLED STUDY; CULTURE MEDIUM; DNA SYNTHESIS; FIBROBLAST CULTURE; HUMAN; HUMAN CELL; IN VIVO STUDY; ISOTOPE TRACING; MUSCLE CELL; PRIORITY JOURNAL;

EID: 0346055119     PISSN: 00260495     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.metabol.2003.08.014     Document Type: Article

References (23)

1. Stryer L: Biochemistry (ed 2). San Francisco, CA, Freeman, 1981
2. Becker MA: Regulation of purine nucleotide synthesis: Effects of inosine on normal and hypoxantine-guanine phosphoribosyltransferase-deficient fibroblasts. Biochim Biophys Acta 435:132-144, 1976
3. D'Mello JPF: Utilization of dietary purines and pyrimidines by non-ruminant animals. Proc Nutr Soc 41:301-308, 1982
4. Lelerko NS, Bronstein A, Munro HN: Effect of dietary purines on de novo synthesis of purine nucleotides in the small intestinal mucosa. Pediatr Res 13:403-408, 1979
5. D'Mello JPF, Peers DG, Whittemore CT: Utilization of dried microbial cells grown on methanol in a semi-purified diet for growing pigs. Br J Nutr 36:403-412, 1976
6. Macallan DC, Fullerton RA, Neese RA, et al: Measurement of cell proliferation by labeling of DNA with stable isotope-labeled glucose: Studies in vitro, in animals, and in humans. Proc Natl Acad Sci USA 95:708-713, 1998
7. Wolfe RR: Radioactive and Stable Isotope Tracers in Biomedicine: Principles and Practice of Kinetics Analysis. New York, NY, Wiley-Liss, 1992
8. Nissim I, Starr SE, Sullivan KE, et al: Rapid method for determining the rate of DNA synthesis and cellular proliferation. Anal Chem 278:198-205, 2000
9. 13C]glycine. Anal Chem 70:1664-1669, 1998
10. Rosenblatt JD, Chinkes DL, Wolfe MH, et al: Stable isotope tracer analysis by GC-MS, including quantification of isotopomer effects. Am J Physiol 263:E584-E596, 1992
11. Zhang XJ, Chinkes DL, Sakurai Y, et al: An isotopic method for measurement of muscle protein fractional breakdown rate in vivo. Am J Physiol 270:E759-E767, 1996
12. Wolfe RR, Herndon DN, Jahoor F, et al: Effect of severe burn injury on substrate cycling by glucose and fatty acids. N Engl J Med 317:403-408, 1987
13. Carraro F, Rosenblatt J, Wolfe RR: Isotopic determination of fibronectin synthesis in humans. Metabolism 40:553-561, 1991
14. Rocha B, Penit C, Baron C, et al: Accumulation of bromodeoxyuridine-labeled cells in central and peripheral lymphoid organs: Minimal estimates of production and turnover rates of mature lymphocytes. Eur J Immunol 20:1697-1708, 1990
15. Asher E, Payne CM, Bernstein C: Evaluation of cell death in EBV-transformed lymphocytes using agarose gel electrophoresis, light microscopy and electron microscopy: II. Induction of non-classic apoptosis ("para-apoptosis") by tritiated thymidine. Leuk Lymphoma 19:107-119, 1995
16. Reichard P: Interactions between deoxyribonucleotide and DNA synthesis. Annu Rev Biochem 57:349-374, 1988
17. 3H]thymidine in studies of the cell cycle. Cell Tissue Kinet 16:51-57, 1983
18. Sprent J, Miller JAFP: Thoracic duct lymphocytes from nude mice: Migratory properties and life-span. Eur J Immunol 2:384-387, 1972
19. Gray D: Population kinetics of rat peripheral B Cells. J Exp Med 167:805-816, 1988
20. Ropke C, Everett NB: Life span of small lymphocytes in the thymolymphatic tissues of normal and thymus-deprived BALA/C mice. Anat Rec 183:89-94, 1975
21. 2O labeling of the deoxyribose moiety of DNA. Proc Natl Acad Sci USA 99:15345-15350, 2002
22. Stout JT, Caskey CT: Hypoxanthine phosphoribosyltransferase deficiency: The Lesch-Nyhan Syndrome and Gouty Arthritis, in Scriver CR, Beaudet AL, Sly WS, et al. (eds): The Metabolic and Molecular Bases of Inherited Disease (ed 6). New York, NY, McGraw-Hill, 1989, pp 1007-1028
23. Murray AW: The biological significance of purine salvage. Annu Rev Biochem 40:811-826, 1971