C-Terminal sequencing of peptide hormones using carboxypeptidase Y and SELDI-TOF mass spectrometry

BioTechniques

Volumn 36, Issue 1, 2004, Pages 32-34

  Cool, David R ^a,b   Hardiman, Atira ^a  

^a WRIGHT STATE UNIVERSITY   (United States)

^b WRIGHT STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYPEPTIDASE C; PEPTIDE HORMONE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ARTICLE; CARBOXY TERMINAL SEQUENCE; DNA MICROARRAY; ENZYME DENATURATION; SEQUENCE ANALYSIS; SURFACE ENHANCED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; TIME OF FLIGHT MASS SPECTROMETRY;

EID: 0346024148     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04361bm01     Document Type: Article

References (12)

1. Issaq, H.J., T.D. Veenstra, T.P. Conrads, and D. Felschow. 2002. The SELDI-TOF MS approach to proteomics: protein profiling and biomarker identification. Biochem. Biophys. Res. Commun. 292:587-592.
2. Cunningham, M.J. 2000. Genomics and Proteomics: the new millennium of drug discovery and development. J. Pharm. Tox. Methods 44:291-300.
3. Jimenez, C.R., K.W. Li, K. Dreisewerd, H.D. Mansvelder, A.B. Brussaard, B.B. Reinhold, R.C. Van der Schors, M. Karas, et al. 1997. Pattern changes of pituitary peptides in rat after salt-loading as detected by means of direct, semiquantitative mass spectrometric profiling. Proc. Natl. Acad. Sci. USA 94:9481-9486.
4. Wright, G.L., L.H. Cazares, S.M. Leung, S. Nasim, B.L. Adam, T.T. Yip, P.F. Schellhammer, L. Gong, and A. Vlahou. 1999. Proteinchip surface enhanced laser desorption/ionization (SELDI) mass spectrometry: a novel protein biochip technology for detection of prostate cancer biomarkers in complex protein mixtures. Prost. Can. Prostat. Dis. 2:264-276.
5. Eggeling, F.V., K. Junker, W. Fiedle, V. Wollscheid, M. Durst, U. Claussen, and G. Ernst. 2001. Mass spectrometry meets chip technology: a new proteomic tool in cancer research? Electrophoresis 22:2898-2902.
6. Loh, Y.P., M.J. Brownstein, and H. Gainer. 1984. Proteolysis in neuropeptide processing and other neural functions. Annu. Rev. Neuroscience 7:189-222.
7. Darby, N.J. and D.G. Smyth. 1990. Endopeptidases and prohormone processing. Biosci. Rep. 10:1-13.
8. Lindberg, I. 1991. The new eukaryotic precursor processing proteinases. Mol. Endocrinol. 5:1361-1365.
9. Seidah, N.G., R. Day, S. Benjannet, N. Rondeau, A. Boudreault, T. Reudelhuber, M.K.-H. Schafer, S.J. Watson, and M. Chretien. 1992. The prohormone and proprotein processing enzymes PC1 and PC2: structure, selective cleavage of mouse POMC and human renin at pairs of basic residues, cellular expression, tissue distribution, and mRNA regulation. NIDA Res. Monogr. 126:132-150.
10. Zhou, A., G. Webb, X. Zhu, and D.F. Steiner. 1999. Proteolytic processing in the secretory pathway. J. Biol. Chem. 274:20745-20748.
11. Boudarine, M., O. Yegorov, A. Sterling-Dubrovsky, L.A. Devi, and Y. Berman. 2002. Developmental changes in opioid peptides and their receptors in Cpe(fat)/Cpe(fat) mice lacking peptide processing enzyme carboxypeptidase E. J. Pharmacol. Exp. Ther. 303: 1317-1324.
12. Patterson, D.H., G.E. Tarr, F.E. Regnier, and S.A. Martin, 1995. C-terminal ladder sequencing via matrix-assisted laser desorption mass spectrometry coupled with carboxypeptidase Y time-dependent and concentration-dependent digestions. Anal. Chem. 67: 3971-3978.