A PCR - Based method for the detection of Tetrahymena corlissi contaminations in Ichthyophthirius multifiliis in vitro cultures

Bulletin of the European Association of Fish Pathologists

Volumn 21, Issue 6, 2001, Pages 222-227

  Pugovkin, Dmitri ^a   Felleisen, Richard ^b   Wahli, Thomas ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b Kantonales Laboratorium Bern   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0345876770     PISSN: 01080288     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Bauer, O.N. (1953). Immunity of fish occurring in infection with Ichthyophthirius multifiliis Fouquet. Doklady Academii nauk SSSR 93, 377.
2. Longo, M.C., Beringer, M.S. and Hartley, J.L. (1990). Use of uracil DNA glycosyase to control carry-over contamination in polymerase chain reaction. Gene 93:125-128.
3. Nielsen CV, Buchmann K. (2000). Prolonged in vitro cultivation of Ichthyophthirius multifiliis using an EPC cell line as substrate. Dis Aquat Organ. Sep 28;42(3):215-9.
4. Felleisen, R. S. J. (1998). Comparative genetic analysis of tritrichomonadid protozoa by Random Amplified Polymorphic DNA (RAPD) technique. Parasitol Res 84, 153-156.
5. Freshney, R.I. (1994). Culture of animal cells. Wiley-Liss, New York, Chichester, Brisbane, Toronto, Singapore.
6. Wahli, T. (1985). Ichthyophthiriasis bei der Forelle. Dissertation, University of Basel, Switzerland.
7. Wright, A.D. and Lynn, D.H. (1995). Phylogeny of the fish parasite lchtyophtirius and its relatives Opliryoglena and Tetrahymena (Ciliophora, Hymenostomatida) inferred from 18S ribosomal RNA sequences Mol.Biol.Evol. 12(2): 285-290.