A comparative evaluation of culture conditions for short-term maintenance (<24 h) of human islets isolated using the Edmonton protocol

Cell and Tissue Banking

Volumn 4, Issue 2-4, 2003, Pages 85-93

  Matsumoto, Shinichi ^a,b,c   Goel, Shilpa ^a   Qualley, Sabrina ^a   Strong, D Michael ^a   Reems, Jo Anna ^a,b  

^a PUGET SOUND BLOOD CENTER   (United States)

^b UNIVERSITY OF WASHINGTON MEDICAL CENTER   (United States)

^c KYOTO UNIVERSITY HOSPITAL   (Japan)

Author keywords

Culture media; Edmonton protocol; Human islets; Islet transplantation; Memphis media

Indexed keywords

BOVINE SERUM ALBUMIN; ENDOTOXIN; INSULIN; SELENIUM; TRANSFERRIN;

ADULT; ARTICLE; CELL COUNT; CLINICAL PROTOCOL; COMPARATIVE STUDY; CONTROLLED STUDY; CULTURE MEDIUM; FEMALE; GRAM STAINING; HUMAN; HUMAN TISSUE; INSULIN DEPENDENT DIABETES MELLITUS; ISOLATION PROCEDURE; MALE; PANCREAS ISLET; PRIORITY JOURNAL; QUALITY CONTROL; TEMPERATURE; TIME; TISSUE CULTURE; TISSUE PRESERVATION; TISSUE TRANSPLANTATION;

EID: 0345832387     PISSN: 13899333     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:CATB.0000007043.15164.8a     Document Type: Article

References (24)

1. Alejandro R., Ferreira J.V., Caulfield A., Froud T., Baidal D., Geiger M., Rothenberg L., Al-Abdullah I.H., Kirlen T., Kenyon N.S. and Ricordi C. 2002. Insulin independence in 7 patients following transplantation of cultured human islets. American Journal of Transplantation 2(Suppl. 3): abstract 358.
2. Anderson A. 1978. Isolated mouse pancreatic islets in culture: Effects of serum and different culture media on the insulin production of the islets. Diabetologia 14: 397-404.
3. Bank H. 1988. Rapid assessment of islet viability with acridine orange and propidium iodide. In Vitro Cell Developmental Biology 24: 266 273.
4. Barnes D. and Sato G. 1980. Methods for growth of cultured cells in serum-free media. Anat. Biochem. 102: 255-270.
5. Brendel M.D., Hering B.J., Schultz A.O. and Bretzel R.G. 2001. International islet transplant registry newsletter #9
6. Di Carlo A., Scharp D.W., Gingerich R.L., Giannarelli R., Ansara M., Olack B.J., Swanson C.J. and Navalesi R. 1994. Insulin and glucagon release from isolated, perifused human islets following low-temperature culture and cryopreservation. Transplantation Proceedings 26: 821-822.
7. Dionne K., Colton C. and Yarmish M. 1993. Effect of hypoxia on insulin secretion by isolated rat and canine islets of Langerhans. Diabetes 42: 12-21.
8. Fraga D., Sabek O., Hathaway D. and Gaber A.O. 1998. A comparison of media supplement method for the extended culture of human islet tissue. Transplantation 65: 1060-1066.
9. Gaber A.O., Fraga D.W., Callicutt C.S., Gerling I.C., Sabek O.M. and Kotb M.Y. 2001. Improved in vivo pancreatic islet function after prolonged in vitro islet culture. Transplantation 72: 1730-1736.
10. Holmes M.A., Clayton H.A., Chadwick D.R., Bell P.R., London N.J. and James R.F. 1995. Functional studies of rat, porcine, and human pancreatic islets cultured in ten commercially available media. Transplantation 60: 854-880.
11. Lacy P.E., Davine J.M. and Finke E.H. 1979. Prolongation of islet allograft survival following in vitro culture (24 °C) and single injection of ALS. Science 2204: 312-313.
12. Lacy P. and Kostianovsky M. 1976. Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16: 35-39.
13. Lakey J.R., Warnock G.L., Kneteman N.M., Ao A. and Rajotte R.V. 1994. Effects of pre-cryopreservation culture on human islet recovery and in vitro function. Transplantation Proceedings 26: 820.
14. Lakey J.R., Warnock G.L., Shapiro A.M., Korbutt G.S., Ao Z., Kneteman N.M. and Rajotte R.V. 1999. Intraductal collagenase delivery into the human pancreas using syringe loading or controlled perfusion. Cell Transplantation 8: 285-292.
15. Matsumoto S., Qualley S.A., Goel S., Hagman D.K., Sweet I.R., Poitout V., Strong D.M., Robertson R.P. and Reems J.A. 2002. Effect of the two-layer (University of Wisconsin solution - Perfluorochemical plus O2) method of pancreas preservation on human islet isolation as assessed by Edmonton isolation protocol. Transplantation 74: 1414-1419.
16. Petkov P., Hahn H.J., Galabova R. and Ziegler M. 1974. Investigations on islets of Langerhans in vitro. Ultrastructure and insulin secretion of isolated rat islets after different digestion with collagenase. Acta Histochemistry (Jena) 51: 50-60.
17. Ricordi C., Lacy P.E., Sterbenz K. and Davie J.M. 1987. Low-temperature culture of human islets or in vivo treatment with L3T4 antibody produces a marked prolongation of islet human-to-mouse xenograft survival. Proceedings of the National Academic Sciences USA 84: 8080-8084.
18. Ricordi C., Gray D.W., Hering B.J., Kaufman D.B., Warnock G.L., Kneteman N.M., Lake S.P., London N.J., Socci C. and Alejandro R., 1990. Islet isolation assessment in man and large animals. Acta Diabetologica Latina 27: 185-195.
19. Ricordi C., Lacy P.E., Finke E.H., Olack B.J. and Sharp D.W. 1988. Automated method for isolation of human pancreatic islets. Diabetes 37: 413-420.
20. Ryan E.A., Lakey J.R.T., Paty B.W., Imes S., Korbutt G.S., Kneteman N.M., Bigam D., Rajotte R.V. and Shapiro A.M. 2002. Successful islet transplantation. Continued insulin reserve provides long-term glycemic control. Diabetes 51: 2148-2157.
21. Ryan E.A., Lakey J.R., Rajotte R.V., Korbutt G.S., Kin T., Imes S., Rabinovitch A., Elliott J.F., Bigam D., Kneteman N.M., Warnock G.L. and Larsen I. 2001. Clinical outcomes and insulin secretion after islet transplantation with the Edmonton protocol. Diabetes 50: 710.
22. Schrezenmeir J., Gero L., Solhdju M., Kirchengessner J., Laue C., Beyer J., Stier H. and Muller-Klieser W. 1994. Relation between secretory function and oxygen supply in isolated islet organs. Transplantation Proceedings 26: 809-813.
23. Shapiro A.M., Lakey J.R., Ryan E.A., Korbutt G.S., Toth E., Warnock G.L., Kneteman N.M. and Rajotte R.V. 2000. Islet transplantation in seven patients with Type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. New England Journal of Medicine 343: 230-238.
24. Sharp D.W., Lacy P.E., Finke E. and Olack B. 1987. Low-temperature culture of human islets isolated by the distention method and purified with Ficoll or Percoll gradients. Surgery 102: 869-879.