메뉴 건너뛰기




Volumn 271, Issue 5253, 1996, Pages 1291-1293

HIV-1 Langerhans' cell tropism associated with heterosexual transmission of HIV

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CLINICAL ARTICLE; HETEROSEXUALITY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; LANGERHANS CELL; NONHUMAN; PRIORITY JOURNAL; SEXUALLY TRANSMITTED DISEASE; VAGINA; VIRUS CELL INTERACTION; VIRUS TRANSMISSION; VIRUS TYPING;

EID: 0345717207     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5253.1291     Document Type: Article
Times cited : (304)

References (38)
  • 1
    • 0009023704 scopus 로고
    • S. Broder, T. C. Mengan Jr., D. Bolognesi, Eds. Williams and Wilkins, Baltimore
    • P. Plot and M. Laga, in Textbook of AIDS Medicine, S. Broder, T. C. Mengan Jr., D. Bolognesi, Eds. (Williams and Wilkins, Baltimore, 1994), pp. 109-132.
    • (1994) Textbook of AIDS Medicine , pp. 109-132
    • Plot, P.1    Laga, M.2
  • 7
    • 0027157883 scopus 로고
    • C. Y. Ou et al., Lancet 341, 1171 (1993)
    • (1993) Lancet , vol.341 , pp. 1171
    • Ou, C.Y.1
  • 9
    • 0028990819 scopus 로고
    • T. A. Peterman, W. Cates Jr., J. N. Wasserheit, in AIDS: Etiology, Diagnosis, Treatment and Prevention, V. T. DeVita Jr et al , Eds (Lippincott, Philadelphia, 1992), pp. 443-451; M. D. C. Guimaraes, A. Munoz, C. Boschi-Pinto, E. A. Castilho, Am. J. Epidemiol. 142, 538 (1995).
    • (1995) Am. J. Epidemiol. , vol.142 , pp. 538
    • Guimaraes, M.D.C.1    Munoz, A.2    Boschi-Pinto, C.3    Castilho, E.A.4
  • 10
    • 0028914972 scopus 로고
    • C. Kunanusont et al., Lancet 345, 1078 (1995).
    • (1995) Lancet , vol.345 , pp. 1078
    • Kunanusont, C.1
  • 12
  • 17
    • 0028236934 scopus 로고
    • LCs were extracted from normal human skin. Epidermal suspension and enrichment of LCs followed the protocol of Teunissen et al. (13), with some modifications [H.-N. Liu, Y.-T Chang, C -K. Wong, Clin. Exp. Dermatol. 19, 113 (1994)]. Skin tissue was digested overnight at 4°C with 2 mg/ml of dispase in RPMI media with 20% fetal calf serum (FCS) and 1% antibiotics. The epidermis was separated and incubated for 15 min with trypsin (0.005%) and deoxyribonuclease (100 U/ml) in phosphate-buffered saline (PBS). The cell suspension was loaded on a discontinuous Ficoll-Metrizoate gradient LCs were recovered from two interfaces, one between the sample and the gradient and the second between layers with densities of 1 068 and 1 057 g/ml. Purity was estimated with immunofluorescence staining with CD1a [OKT6 (Ortho)], CD3, and CD14. The cells were maintained in RPMI media with 20% FCS and 1% antibiotics. No clumping of LCs with keratinocytes was observed. The viability according to Trypan Blue exclusion was higher than 85% for the period of observation in the assay.
    • (1994) Clin. Exp. Dermatol. , vol.19 , pp. 113
    • Liu, H.-N.1    Chang, Y.-T.2    Wong, C.-K.3
  • 18
    • 0023055561 scopus 로고
    • The classification system is detailed in Centers for Disease Control (CDC), Morb. Mortal. Wkly. Rep. 35, 334 (1986).
    • (1986) Morb. Mortal. Wkly. Rep. , vol.35 , pp. 334
  • 20
    • 13344293549 scopus 로고    scopus 로고
    • note
    • PBMCs from HIV-1-seronegative individuals were purified by the Ficoll-Hypaque method, stimulated with 10 μg/ml of phytohemagglutinin A (PHA) (Sigma) for 72 hours, and maintained in RPMI 1640 with 20% fetal bovine serum, 10 U/ml of recombinant human interleukin-2, 100 U/ml of penicillin, and 100 μg/ml of streptomycin.
  • 21
    • 13344265554 scopus 로고    scopus 로고
    • note
    • 6 PBMCs per cubic centimeter and RPMI 1640 plus 5% (v/v) human serum and 20% fetal bovine serum. After 5 days, cells were washed twice with PBS and maintained with RPMI 1640 with 20% fetal bovine serum, 100 U/ml of penicillin, and 100 μg/ml of streptomycin.
  • 22
    • 13344254362 scopus 로고    scopus 로고
    • note
    • 6 cells/ml in the same media as for MDMs and PMBCs.
  • 23
    • 13344257608 scopus 로고    scopus 로고
    • note
    • 6 MT-2 cells were challenged with 1 ng/ml of p24 from each isolate on day 1. All the LC assays were done 24 hours after the extraction and enrichment of the cells Assays with MDMs were started 4 days after the removal of nonadherent cells. Cell cultures were inoculated with each isolate for 18 hours at 37°C, washed with PBS, and resuspended in fresh complete media. Infections with PBMCs, MDMs, and MT-2 cells were done twice, and LC infections were done in duplicate with at least two different LC preparations To determine p24 levels, we obtained culture supematants by removing cells and cell debris after centrifugation at 3500 rpm for 10 min from LCs, PBMCs, and MDMs on days 7, 14, and 21. Standard dilutions were done in each sample, and the procedure was performed according to the manufacturer's protocol (NEN-Dupont Core profile enzymelinked immunosorbent assay).
  • 24
    • 13344264502 scopus 로고    scopus 로고
    • unpublished results
    • L Soto-Ramirez et al., unpublished results.
    • Soto-Ramirez, L.1
  • 28
    • 0027413865 scopus 로고
    • A. Gianetti et al., J. Acquired Immune Defic. Syndr. 6, 329 (1993); M. Henry, A. Uthman, C. Ballaun, G. Stingl, E. Tschachler, J. Invest. Dermatol. 103, 593 (1994).
    • (1993) J. Acquired Immune Defic. Syndr. , vol.6 , pp. 329
    • Gianetti, A.1
  • 30
    • 0024464463 scopus 로고
    • C. J. Miller et al., J. Virol. 63, 4277 (1989); C. J. Miller et al , Am. J. Pathol 141, 655 (1992); C. J. Miller et al., Lab Invest. 70, 225 (1994).
    • (1989) J. Virol. , vol.63 , pp. 4277
    • Miller, C.J.1
  • 31
    • 0026669799 scopus 로고
    • C. J. Miller et al., J. Virol. 63, 4277 (1989); C. J. Miller et al , Am. J. Pathol 141, 655 (1992); C. J. Miller et al., Lab Invest. 70, 225 (1994).
    • (1992) Am. J. Pathol , vol.141 , pp. 655
    • Miller, C.J.1
  • 32
    • 0024464463 scopus 로고
    • C. J. Miller et al., J. Virol. 63, 4277 (1989); C. J. Miller et al , Am. J. Pathol 141, 655 (1992); C. J. Miller et al., Lab Invest. 70, 225 (1994).
    • (1994) Lab Invest. , vol.70 , pp. 225
    • Miller, C.J.1
  • 34
    • 0026099461 scopus 로고
    • F. A. Plummer, J. N. Sirnonsen, D. W. Cameron, J. Infect. Dis. 163, 233 (1991); European Study Group, Br. Med. J 304, 809 (1992); K. A. Hamed et al., J. Infect. Dis. 167, 798 (1993); A. Nicolosi, M. Musicco, A. Saracco, A. Lazzarin, J. Acquired Immune Defic. Syndr. 7, 296 (1994).
    • (1991) J. Infect. Dis. , vol.163 , pp. 233
    • Plummer, F.A.1    Sirnonsen, J.N.2    Cameron, D.W.3
  • 35
    • 0026562144 scopus 로고
    • F. A. Plummer, J. N. Sirnonsen, D. W. Cameron, J. Infect. Dis. 163, 233 (1991); European Study Group, Br. Med. J 304, 809 (1992); K. A. Hamed et al., J. Infect. Dis. 167, 798 (1993); A. Nicolosi, M. Musicco, A. Saracco, A. Lazzarin, J. Acquired Immune Defic. Syndr. 7, 296 (1994).
    • (1992) Br. Med. J , vol.304 , pp. 809
  • 36
    • 0027523209 scopus 로고
    • F. A. Plummer, J. N. Sirnonsen, D. W. Cameron, J. Infect. Dis. 163, 233 (1991); European Study Group, Br. Med. J 304, 809 (1992); K. A. Hamed et al., J. Infect. Dis. 167, 798 (1993); A. Nicolosi, M. Musicco, A. Saracco, A. Lazzarin, J. Acquired Immune Defic. Syndr. 7, 296 (1994).
    • (1993) J. Infect. Dis. , vol.167 , pp. 798
    • Hamed, K.A.1
  • 37
    • 0028181255 scopus 로고
    • F. A. Plummer, J. N. Sirnonsen, D. W. Cameron, J. Infect. Dis. 163, 233 (1991); European Study Group, Br. Med. J 304, 809 (1992); K. A. Hamed et al., J. Infect. Dis. 167, 798 (1993); A. Nicolosi, M. Musicco, A. Saracco, A. Lazzarin, J. Acquired Immune Defic. Syndr. 7, 296 (1994).
    • (1994) J. Acquired Immune Defic. Syndr. , vol.7 , pp. 296
    • Nicolosi, A.1    Musicco, M.2    Saracco, A.3    Lazzarin, A.4
  • 38
    • 13344274287 scopus 로고    scopus 로고
    • note
    • We acknowledge support of the research staff of the Fenway Community Health Center, especially R. Goldstein. This research was supported in part by grants CA 39805 and HL 33774 from NIH and by a grant from the Massachusetts Department of Public Health L.E S.-R, R S., C.A., and P.A were supported by training grant 5 D43 TW0004 from the Fogarty International Center, NIH. The authors thank K. MacArthur for editorial assistance.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.