Interference of thiomersal in biologicals during protein estimation

Biologicals

Volumn 30, Issue 4, 2002, Pages 271-275

  Dogar, Vikas ^a   Sokhey, Jaspal ^b   Kumar, Sanjeev ^a   Sood, Dinesh Kumar ^a   Tahlan, Ajay Kumar ^a  

^a CENTRAL DRUG RESEARCH INSTITUTE   (India)

^b VSQ   (India)

Author keywords

Biologicals; Hepatitis B vaccine; Interference; Lowry's protein assay; Protein estimation; Thiomersal

Indexed keywords

HEPATITIS B VACCINE; PHENOL; REAGENT; THIOMERSAL;

ANALYTIC METHOD; ARTICLE; CALCULATION; COLOR; CONCENTRATION RESPONSE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONTENT; PROTEIN DETERMINATION; STATISTICAL SIGNIFICANCE;

EID: 0345701516     PISSN: 10451056     EISSN: None     Source Type: Journal    
DOI: 10.1006/biol.2002.0331     Document Type: Article

References (14)

1. British Pharmacopoeia, Hepatitis-B Vaccines (r-DNA), 1993, 1923, 925.
2. European Pharmacopoeia, Hepatitis-B Vaccine (r-DNA), 1997, 940, 942.
3. Folin O., Ciocalteu V. On tyrosine and tryptophane determination in proteins. J Biol Chem. 73:1927;627-650.
4. Indian Pharmacopoeia, Hepatitis-B Virus vaccine, Vol. 1, 1996.
5. Lowry O.H., Rosenbrough N.J., Farr A.L., and Randal R.J. Protein measurement with Folin Phenol reagent. J Biol Chem. 193:1951;265-275.
6. Peterson G.L. Review of the Folin Phenol Protein Quantitation method of Lowry, Rosenbrough, Farr and Randall. Analytical Biochemistry. 100:1979;201-220.
7. Polacheck I., Cabib E. A simple procedure for protein determination by the Lowry Method in dilute solutions and in the presence of interfering substances. Analytical Biochemistry. 117:1981;311-314.
8. Scopes R.K. Methods of measuring protein concentration. Scopes R.K. Protein purification: Principles and Practices. 1988;278-283 Springer Verlag.
9. Sodium Salt of 2 (Ethytlmercuriothiobenzoic acid), Sigma chemical company, U.S.A. 1995.
10. WHO, Manual for the production and control of vaccines.
11. WHO, Manual for the production and control of vaccines, Tetanus toxoid: Test for thiomersal content.
12. WHO, Manual for the production and control of vaccines.
13. TRS. 786:1989;38-71.
14. TRS. 858:1995;63-75.