Recombinant human liver betaine-homocysteine S-methyltransferase: Identification of three cysteine residues critical for zinc binding

Biochemistry

Volumn 38, Issue 42, 1999, Pages 13991-13998

  Breksa III, Andrew P ^a   Garrow, Timothy A ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BINDING ENERGY; CATALYSIS; CHEMICAL BONDS; ESCHERICHIA COLI; MUTAGENESIS; OXIDATION; PROTEINS; PURIFICATION; REDUCTION; UROLOGY; ZINC;

BETAINE; BETAINE HOMOCYSTEINE METHYLTRANSFERASE; CYSTEINE; DIMETHYLGLYCINE; METHIONINE; METHYLMETHANE THIOSULFONATE;

ENZYMES;

METHYLTRANSFERASE; RECOMBINANT ENZYME; ZINC ION;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; ENZYME MECHANISM; ESCHERICHIA COLI; HUMAN; METAL BINDING; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION;

BASE SEQUENCE; BETAINE-HOMOCYSTEINE S-METHYLTRANSFERASE; BINDING SITES; CHITIN; CYSTEINE; DNA GYRASE; DNA TOPOISOMERASES, TYPE II; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; HUMANS; LIVER; METHYLTRANSFERASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SOFTWARE; ZINC;

EID: 0345624497     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi991003v     Document Type: Article

References (16)

1. McKeever, M. P., Weir, D. G., Molloy, A., and Scott, J. M. (1991) Clin. Sci. 81, 551-556.
2. Finkelstein, J. D., Harris, B. J., and Kyle, W. E. (1972) Arch. Biochem. Biophys. 153, 320-324.
3. Lee, K.-Y., Cava, M., Amiri, P., Ottoboni, T., and Lindquist, R. N. (1992) Arch. Biochem. Biophys. 292, 77-86.
4. Sunden, L. F. S., Renduchintala, M. S., Park, E. I., Miklasz, S. D., and Garrow, T. A. (1997) Arch. Biochem. Biophys. 345, 171-174.
5. Garrow, T. A. (1996) J. Biol. Chem. 271, 22831-22838.
6. Goulding, C. W., and Matthews, R. G. (1997) Biochemistry 36, 15749-15757.
7. Millian, N. S., and Garrow, T. A. (1998) Arch. Biochem. Biophys. 356, 93-98.
8. Peariso, K., Goulding, C. W., Huang, S., Matthews, R. G., and Pennerhahn, J. E. (1998) J. Am. Chem. Soc. 120, 8410-8416.
9. Vogel, H. J, and Bonner, D. M. (1959) J. Biol. Chem. 218, 97-106.
10. Rao, P. V., Garrow, T. A., John, F., Garland, D., Millian, N. S., and Zigler, J. S. (1998) J. Biol. Chem. 273, 30669-30674.
11. Zheng, Z., Schäffer, A. A., Miller, W., Madden, T. L., Lipman, D. J., Koonin, E. V., and Altschul, S. F. (1998) Nucleic Acids Res. 26, 3986-3990.
12. Skiba, W. E., Taylor, M. P., Wells, M. S., Mangum, J. H., and Awad, W. M. (1982) J. Biol. Chem. 257, 14944-14948.
13. Wilker, J. J., and Lippard, S. J. (1995) J. Am. Chem. Soc. 117, 8682-8683.
14. Neuhiel, B., Thanbichler, M., Lottspeich, F., and Bock, A. (1999) J. Biol. Chem. 274, 5407-5414.
15. Park, E. I., and Garrow, T. A. (1999) J. Biol. Chem. 274, 7816-7824.
16. Ericson, L. E. (1960) Acta Chem. Scand. 14, 2102-2112.