Analysis of T-cell receptor γ gene rearrangements by PCR-genescan and PCR-polyacrylamide gel electrophoresis in early-stage mycosis fungoides/large-plaque parapsoriasis [6]

Dermatology

Volumn 207, Issue 4, 2003, Pages 418-419

  Costa, Carlota ^a   Gallardo, Fernando ^a   Bellosillo, Beatriz ^a   Espinet, Blanca ^a   Pujol, Ramón M ^a   Barranco, Carlos ^a   Serrano, Sergi ^a   Solé, Francesc ^a  

^a HOSPITAL DEL MAR   (Spain)

Author keywords

Early stages of mycosis fungoides; Genescan; Parapsoriasis; Polyacrylamide gel electrophoresis; Polymerase chain reaction; T cell receptor chain

Indexed keywords

ADULT; AGED; ANALYTIC METHOD; ANAMNESIS; CLINICAL ARTICLE; CLINICAL FEATURE; ERYTHEMA; FEMALE; FLUORESCENCE; GENE AMPLIFICATION; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; LETTER; LYMPHOCYTE CLONE; LYMPHOCYTIC INFILTRATION; LYMPHOPROLIFERATIVE DISEASE; MALE; MYCOSIS FUNGOIDES; PARAPSORIASIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RASH; RECEPTOR GENE; SYMPTOM; T LYMPHOCYTE RECEPTOR GAMMA GENE;

AGED; CLONE CELLS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; GENE REARRANGEMENT, GAMMA-CHAIN T-CELL ANTIGEN RECEPTOR; GENES, T-CELL RECEPTOR GAMMA; HUMANS; MALE; MIDDLE AGED; MYCOSIS FUNGOIDES; PARAPSORIASIS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SKIN NEOPLASMS;

EID: 0345600154     PISSN: 10188665     EISSN: None     Source Type: Journal    
DOI: 10.1159/000074131     Document Type: Letter

References (10)

1. Kikuchi A, Naka W, Harada T, Sakuraoka K, Harada R, Nishikawa T: Parapsoriasis in plaque: Its potential for progression to malignant lymphoma. J Am Acad Dermatol 1993;29:419-422.
2. Haeffner AC, Smoller BR, Zepter K, Wood GS: Differentiation and clonality of lesional lymphocytes in small plaque parapsoriasis. Arch Dermatol 1995;131:321-324.
3. Muche M, Lukowsky A, Asadullah K, Gellerich S, Sterry W: Demonstration of frequent occurrence of clonal T-cells in the peripheral blood of patients with primary cutaneous T-cell lymphoma. Blood 1997;4:1636-1642.
4. Scheller U, Muche M, Sterry W, Lukowsky A: Detection of clonal T cells in cutaneous T cell lymphoma by polymerase chain reaction: Comparison of mutation detection enhancement-polyacrylamide gel electrophoresis, temperature gradient gel electrophoresis and fragment analysis of sequencing gels. Electrophoresis 1998;19:653-658.
5. Muche M, Lukowsky A, Heim J, Friedrich M, Audring H, Sterry W: Demonstration of frequent occurrence of clonal T cells in the peripheral blood but not in the skin of patients with small plaque parapsoriasis. Blood 1999;94:1409-1417.
6. Stachowitz S, Mempel M, Schmöckel C, von Spanyl R, Abeck D: Variable course of patients with plaque psoriasis: Lack of transformation into tumorous mycosis fungoides. Blood 2000;95:3635-3636.
7. Klemke CD, Dippel E, Dembinski A, Ponitz N, Assaf C, Hummel M, Stein H, Goerdt S: Clonal T cell receptor γ-chain gene rearrangement by PCR-based GeneScan analysis in the skin and blood of patients with parapsoriasis and early stage mycosis fungoides. J Pathol 2002;197:348-354.
8. Dippel E, Assaf C, Hummel M, Schrag H, Stein H, Goerdt S, Orfanos C: Clonal T-cell receptor γ-chain gene rearrangement by PCR-based Genescan analysis in advanced cutaneous T-cell lymphoma: A critical evaluation. J Pathol 1999;188:146-154.
9. Assaf C, Hummel M, Dippel E, Goerdt S, Müller H, Anagnostopoulos I, Orfanos CE, Stein H: High detection rate of T-cell receptor beta chain rearrangement in T-cell lymphoproliferations by family specific polymerase chain reaction in combination with the GeneScan technique and DNA sequencing. Blood 2000;96:640-646.
10. Gutzmer R, Mommert S, Kiehl P, Wittmann M, Kapp A, Werfel T: Detection of clonal T cell receptor γ gene rearrangements in cutaneous T cell lymphoma by Lightcycler-polymerase chain reaction. J Invest Dermatol 2001;116:926-932.