Resolution and identification of G(q)/G11α and G(i)α/G(o)α proteins in brown adipose tissue: Effect of cold acclimation

Journal of Molecular Endocrinology

Volumn 23, Issue 2, 1999, Pages 223-229

  Bourova L ^a,b   Novotny J ^a,b   Svoboda, P ^a,b  

^a INSTITUTE OF PHYSIOLOGY   (Czech Republic)

^b CHARLES UNIVERSITY   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; PHOSPHOLIPASE C;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN MICROSOME; BROWN ADIPOSE TISSUE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; COLD ACCLIMATIZATION; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; RAT; RECEPTOR UPREGULATION; ADAPTATION; AMINO ACID SEQUENCE; ANIMAL; CELL LINE; CHO CELL; COLD; HAMSTER; HUMAN; METABOLISM; MITOCHONDRION; PHYSIOLOGY;

ADAPTATION, PHYSIOLOGICAL; ADIPOSE TISSUE, BROWN; AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CHO CELLS; COLD; CRICETINAE; GTP-BINDING PROTEINS; HUMANS; MITOCHONDRIA;

EID: 0345426368     PISSN: 09525041     EISSN: None     Source Type: Journal    
DOI: 10.1677/jme.0.0230223     Document Type: Article

References (35)

1. 3H]ouabain binding and Na,K-ATPase activity in brown adipose tissue plasma membranes of cold-acclimated hamsters. Journal of Molecular Endocrinology 22 55-64.
2. Bronnikov G, Houštek J & Nedergaard J 1992 β-Adrenergic, cAMP mediated stimulation of proliferation of brown fat cells in primary culture. Journal of Biological Chemistry 267 2006-2013.
3. oα in rat sympathetic neurones. Journal of Physiology 477415-422.
4. Chaudhry A & Granneman JG 1991 Developmental changes in adenylyl cyclase and GTP binding proteins in brown fat. American Journal of Physiology 261 R403-R411.
5. sα activity. American Journal of Physiology 263 R34-R38.
6. i3α in control of cell proliferation. Cellular Signalling 8 225-234.
7. De Pergola G, Xu X, Carlsson B, Eriksson P, Giorgino R & Bjorntorp P 1994 Messenger RNA of G proteins alpha-subunit in rat white adipose tissue. International Journal of Obesity 18 269-272.
8. Gierschik P, Milligan G, Pines M, Goldsmith P, Codina J, Klee W & Spiegel A 1986 Use of specific antibodies to quantitate the guanine-nucleotide binding protein Go in brain. Proceedings of the National Academy of Sciences of the USA 83 2258-2262.
9. Goldsmith P, Backlund PS, Rossiter K, Carter A, Milligan G, Unson CG & Spiegel A 1988 Purification of heterotrimeric GTP-binding proteins from brain: identification of a novel form of Go. Biochemistry 27 7085-7090.
10. sα protein in rat brown adipose tissue. Endocrinology 127 1596-1601.
11. Hinsch K-D, Rosenthal W, Spicher K, Binder T, Gausepohl H, Frank R, Schulz G & Joost HG 1988 Adipocyte plasma membranes contain two Gi subtypes but are devoid of Go. FEBS Letters 238 191-196.
12. Kim G-D, Carr IC, Anderson LA, Zabavnik J, Eidne KA & Milligan G 1994 The long isoform of the rat thyrotropin-releasing hormone receptor down-regulates Gq proteins. Journal of Biological Chemistry 269 19933-19940.
13. 1 particle and reconstruction of succinate-cytochrome c reductase. Methods in Enzymology 10 216-225.
14. Milligan G 1990 Immunological probes and the identification of guanine nucleotide binding proteins. In G Proteins as Mediators of Cellular Signalling Processes, pp 31-46. Eds MD Houslay & G Milligan, London: John Wiley & Sons Ltd.
15. Mitchell FM, Griffiths SL, Saggerson ED, Houslay MD, Knowler JT & Milligan G 1989 Guanine-nucleotide-binding proteins expressed in rat white adipose tissue. Identification of both mRNAs and proteins corresponding to Gi1, Gi2 and Gi3. Biochemical Journal 262 403-408.
16. Mitchell FM, Mullaney I, Godfrey PP, Arkinstall SJ, Wakelam MJO & Milligan G 1991 Widespread distribution of Gq/G11 alpha detected immunologically by an antipeptide antiserum directed against the predicted C-terminal decapeptide. FEBS Letters 287 171-174.
17. Mohell N, Wallace M & Fain JN 1984 Alpha1-adrenergic stimulation of phosphatidylinositol turnover and respiration of brown fat cells. Molecular Pharmacology 25 64-69.
18. o in neuroblastoma × glioma hybrid cells: independent regulation during cyclic AMP-induced differentiation. Journal of Neurochemistry 55 1890-1897.
19. Mullaney I, Dodd MW, Buckley N & Milligan G 1993a Agonist activation of transfected human M1 muscarinic acetylcholine receptors in CHO cells results in down-regulation of both the receptor and the alpha subunit of the G protein Gq. Biochemical Journal 289 125-131.
20. Mullaney I, Mitchell FM, McCallum JF, Buckley NJ & Milligan G 1993e The human muscarinic M1 acetylcholine receptor when expressed in CHO cells, activates and down-regulates both Gq and G11 alpha equally and non-selectively. FEBS Letters 324 241-245.
21. + dependent. Biochimica et Biophysica Acta 930 438-445.
22. Nanberg E & Putney J 1986 Alpha1-adrenergic activation of brown adipocytes leads to an increased formation of inositol phosphates. FEBS Letters 195 319-322.
23. Novotný J, Kvapil P, Bokoch GM & Ransnas L 1995 Isoproterenol-induced subcellular redistribution of G protein beta-subunits in S49 lymphoma cells demonstrated by a novel competitive ELISA. Archives of Physiology and Biochemistry 103 202-210.
24. Rapiejko PJ, Watkins PJ, Ros M & Malbon CC 1990 G protein subunit mRNA levels in rat heart, liver and adipose tissue: analysis by DNA-excess solution hybridization. Biochimica et Biophysica Acta 1052 348-350.
25. Rehnmark S, Nechad M, Herron D, Cannon B & Nedergaard J 1990 α- and β-Adrenergic induction of expression of the uncoupling protein thermogenin in brown adipose tissue differentiated in culture. Journal of Biological Chemistry 265 16464-16471.
26. Schimmel RJ & Elliot ME 1986 Pertussis toxin does not prevent α-adrenergic stimulated breakdown of phosphoinositides or respiration in brown adipocytes. Biochemical and Biophysical Research Communications 135 823-829.
27. Strathmann M & Simon MI 1990 G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates. Proceedings of the National Academy of Sciences of the USA 87 9113-9117.
28. Svoboda P & Milligan G 1994 Agonist-induced transfer of the alpha subunits of the guanine-nucleotide-binding regulatory proteins Gq and G11 and of muscarinic m1 acetylcholine receptors from plasma membranes to a light-vesicular membrane fraction. European Journal of Biochemistry 224 455-462.
29. Svoboda P & Mosinger B 1981 Catecholamines and the brain microsomal Na,K-adenosine-triphonphatase I. Protection against lipoperoxidative damage. Biochemical Pharmacology 30 433-439.
30. Svoboda P, Unnelius L, Cannon B & Nedergaard J 1993 Attenuation of Gs alpha coupling efficiency in brown-adipose tissue plasma membranes from cold-acclimated hamsters. Biochemical Journal 295 655-661.
31. Svoboda P, Unnelius L, Dicker A, Cannon B & Nedergaard J 1996a Cold-induced reduction in Gi alpha proteins in brown adipose tissue. Effects of cellular hypersensitization to noradrenaline caused by pertussis-toxin treatment. Biochemical Journal 313 761-768.
32. Svoboda P, Kim G-D, Crassie MA, Eidne KA & Milligan G 1996b Thyrotropin-releasmg hormone-induced subcellular redistribution and down-regulation of G11 alpha: analysis of agonist regulation of coexpressed G11 alpha species variants. Molecular Pharmacology 49 646-655.
33. Watkins DC, Rapiejko PJ, Roc M, Wang H-Y & Malbon CC 1989 G-protein mRNA levels during adipocyte differentiation. Biochemical and Biophysical Research Communications 165 929-934.
34. 2+ levels in brown adipocytes. Biochemical and Biophysical Research Communications 163 292-300.
35. Xu X, De Pergola G, Eriksson P, Fu L, Carlsson B, Yang S, Eden S & Bjorntorp P 1993 Post-receptor events involved in the up-regulation of beta-adrenergic receptor mediated lipolysis by testosterone in rat white adipocytes. Endocrinology 132 1651-1657.