High-level expression and purification of human xylosyltransferase I in High Five insect cells as biochemically active form

Biochemical and Biophysical Research Communications

Volumn 312, Issue 3, 2003, Pages 537-544

  Kuhn, Joachim ^a   Müller, Sandra ^a   Schnölzer, Martina ^b   Kempf, Tore ^b   Schön, Sylvia ^a   Brinkmann, Thomas ^a   Schöttler, Manuela ^a   Götting, Christian ^a   Kleesiek, Knut ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Glycosyltransferase; Insect cells; Perfusion chromatography; Proteoglycan

Indexed keywords

ANTIBODY; CHELATE; ENZYME; GLYCINE; GLYCOSYLTRANSFERASE; HEPARIN; HISTIDINE; ISOLEUCINE; NICKEL; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; TRANSFERASE; TRYPSIN; UDP XYLOSE PROTEIN XYLOSYLTRANSFERASE; UDP XYLOSE-PROTEIN XYLOSYLTRANSFERASE; ULINASTATIN; UNCLASSIFIED DRUG; VALINE; XYLOSE; XYLOSYLTRANSFERASE I;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANIMAL; ANIMAL CELL; ARTICLE; BIOSYNTHESIS; CATALYSIS; CELL CULTURE; CELL LINE; CHEMISTRY; ENZYME ACTIVATION; ENZYME PURIFICATION; GENE EXPRESSION REGULATION; GENETICS; HUMAN; INSECT CELL; INSECT CELL CULTURE; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR WEIGHT; MOTH; NONHUMAN; PERFUSION; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; QUALITATIVE ANALYSIS; QUALITY CONTROL; SOLUBILITY; SUPERNATANT; SUSPENSION CELL CULTURE; TECHNIQUE;

INSECTA;

EID: 0345415155     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2003.10.157     Document Type: Article

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