The DrrC protein of Streptomyces peucetius, a UvrA-like protein, is a DNA-binding protein whose gene is induced by daunorubicin

FEMS Microbiology Letters

Volumn 168, Issue 2, 1998, Pages 243-249

  Furuya, Kaoru ^a   Richard Hutchinson, C ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Anthracycline; Antibiotic resistance; Antitumor antibiotic; Daunorubicin; DNA binding protein; Doxorubicin; Streptomyces peucetius

Indexed keywords

ADENOSINE TRIPHOSPHATE; DAUNORUBICIN; DNA BINDING PROTEIN;

ARTICLE; DNA REPAIR; DRUG SYNTHESIS; ESCHERICHIA COLI; GENE INDUCTION; IMMUNOBLOTTING; PRIORITY JOURNAL; PROMOTER REGION; STREPTOMYCES;

ADENOSINE TRIPHOSPHATASES; ANTIBIOTICS, ANTINEOPLASTIC; BACTERIAL PROTEINS; BLOTTING, WESTERN; DAUNORUBICIN; DNA, BACTERIAL; DNA-BINDING PROTEINS; DRUG RESISTANCE, MICROBIAL; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; PROTEIN FOLDING; STREPTOMYCES; TRANSCRIPTION FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STREPTOMYCES PEUCETIUS;

EID: 0345404399     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00432-7     Document Type: Article

References (29)

1. Guilfoile, P.G. and Hutchinson, C.R. (1991) A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. Proc. Natl. Acad. Sci. USA 88, 8553-8557.
2. Higgins, C.F., Hiles, I.D., Salmond, G.P.C., Gill, D.R., Downie, J.A., Evans, I.J., Holland, I.B., Gray, L., Buckel, S.D., Bell, A.W. and Hermodson, M.A. (1986) A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria. Nature 323, 448-450.
3. Higgins, C.F. 1991. Molecular basis of multidrug resistance mediated by P-glycoprotein. Curr. Opin. Biotechnol. 2, 278-281.
4. Lomovskaya, N., Hong, S.K., Kim, S.U., Fonstein, L., Furuya, K. and Hutchinson, C.R. (1996) The Streptomyces peucetius drrC gene encodes a UvrA-like protein involved in daunorubicin resistance and production. J. Bacteriol. 178, 3238-3245.
5. Friedberg, E.C. (1985) DNA Repair, Freeman, New York.
6. Kacinski, B.M. and Rupp, W.D. (1984) Interactions of the UvrABC endonuclease in vivo and in vitro with DNA damage produced by antineoplastic anthracyclines. Cancer Res. 44, 3489-3492.
7. 2 locus. J. Bacteriol. 177, 1216-1224.
8. Furuya, K. and Hutchinson, C.R. (1996) The DnrN protein of Streptomyces peucetius, a pseudo-response regulator, is a DNA-binding protein involved in the regulation of daunorubicin biosynthesis. J. Bacteriol. 178, 6310-6318.
9. Stutzman-Engwall, K.J., Otten, S.L. and Hutchinson, C.R. (1992) Regulation of secondary metabolism in Streptomyces: its use for the overproduction of daunorubicin in Streptomyces peucetius. J. Bacteriol. 174, 144-154.
10. Tang, L., Grimm, A., Zhang, Y.-X. and Hutchinson, C.R. (1996) Purification and characterization of the DnrI DNA-binding protein, a transcriptional activator for daunorubicin biosynthesis in Streptomyces peucetius. Mol. Microbiol. 22, 801-813.
11. 1 locus that controls daunorubicin biosynthesis in Streptomyces peucetius. J. Bacteriol. 177, 1208-1215.
12. Vara, J.A., Lewandowska-Skarbek, M., Wang, Y.-G., Donadio, S. and Hutchinson, C.R. (1989) Cloning of genes governing the deoxysugar portion of the erythromycin biosynthetic pathway in Saccharopolyspora erythraea (Streptomyces erythreus). J. Bacteriol. 171, 5872-5881.
13. Sambrook, J., Frisch, E.F. and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. Vols. 1-3. Cold Spring Harbor Press, Cold Spring Harbor, New York.
14. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72, 248-254.
15. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature 227, 680-685.
16. Claassen, L.A. and Grossman, L. (1991) Deletion mutagenesis of the Escherichia coli UvrA. J. Biol. Chem. 266, 11388-11394.
17. Chodosh, L.A. (1994) Mobility shift DNA-binding assay using gel electrophoresis. In: Current Protocols in Molecular Biology, pp. 12.2.1-12.2.7. John Wiley and Sons, New York.
18. TGC sequences revealed by footprinting titration experiments. Biochemistry 29, 6145-6153.
19. Grimm, A., Madduri, K., Ali, A. and Hutchinson, C.R. (1994) Characterization of the Streptomyces peucetius ATCC 29050 genes encoding doxorubicin polyketide synthase. Gene 151, 1-10.
20. Bibb, M.J., White, J., Ward, J.M. and Janssen, G.R. (1994) The mRNA for the 23S rRNA methylase encoded by the ermE gene of Saccharopolyspora erythraea is translated in the absence of a conventional ribosome-binding site. Mol. Microbiol. 14, 533-545.
21. Kaur, P. (1997) Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein. J. Bacteriol. 179, 569-575.
22. Kaur, P. and Russell, J. (1998) Biochemical coupling between the DrrA and DrrB proteins of the doxorubicin efflux pump of Streptomyces peucetius. J. Biol. Chem. 273, 17933-17939.
23. Seeberg, E. and Steinum, A.-L. (1982) Purification and properties of the uvrA protein from Escherichia coli. Proc. Natl. Acad. Sci. USA 79, 988-992.
24. Selby, C.P. and Sancar, A. (1991) Noncovalent drug-DNA binding interactions that inhibit and stimulate (A)BC exconuclease. Biochemistry 30, 3841-3849.
25. Feinstein, E., Canaani, E. and Weiner, L.M. (1993) Dependence of nucleic acid degradation on in situ free-radical production by adriamycin. Biochemistry 32, 13156-13161.
26. Tang, M.S., Nazimiec, M.E., Doisy, R.P., Pierce, J.R., Hurley, L.H. and Alderete, B.E. (1991) Repair of helix-stabilizing anthramycin-N2 guanine DNA adducts by UvrA and UvrB proteins. J. Mol. Biol. 220, 855-866.
27. Ali, A. and Hutchinson, C.R., unpublished results.
28. August, P.R., Flickinger, M.C. and Sherman, D.H. (1994) Cloning and analysis of a locus (mcr) involved in mitomycin C resistance in Streptomyces lavendulae. J. Bacteriol. 176, 4448-4454.
29. Johnson, D.A., August, P.R., Shackleton, C., Liu, H.-W. and Sherman, D.H. (1997) Microbial resistance to mitomycins involves a redox relay mechanism. J. Am. Chem. Soc. 119, 2576-2586.