Separation and purification of glucoamylase in aqueous two-phase systems by a two-step extraction

Journal of Chromatography B: Biomedical Applications

Volumn 711, Issue 1-2, 1998, Pages 309-312

  Miyuki Minami, Nancy ^a   Vahan Kilikian, Beatriz ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Aqueous two phase systems; Enzymes; Glucoamylase

Indexed keywords

GLUCAN 1,4 ALPHA GLUCOSIDASE; MACROGOL; MACROGOL 4000; MACROGOL 6000; POTASSIUM DIHYDROGEN PHOSPHATE;

AQUEOUS SOLUTION; ARTICLE; ENZYME PURIFICATION; PARTITION COEFFICIENT; PHASE SEPARATION; PRIORITY JOURNAL;

CHEMISTRY, ANALYTICAL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLUCAN 1,4-ALPHA-GLUCOSIDASE; PHOSPHATES; POLYETHYLENE GLYCOLS; POTASSIUM COMPOUNDS; WATER;

EID: 0345404203     PISSN: 15726495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4347(98)00039-5     Document Type: Article

References (7)

1. Pandey A. Starch. 47:1995;439.
2. P.A. Albertsson, Partition of Cell Particles and Macromolecules, Wiley, New York, 1986.
3. J.A. Asenjo, Separation Processes in Biotechnology, Marcel Dekker, New York, 1990.
4. G. Johansson, in D.E. Brooks, H. Walter, D. Fisher (Editors), Partitioning in Aqueous Two Phase Systems, Academic Press, Orlando, FL, 1985.
5. Bradford M.M. Anal. Biochem. 72:1976;248.
6. Córdova-Lopez J., Gutiérrez-Rojas M., Huerta S., Sarcedo-Castañeda G., Favela-Torres E. Biotech. Techn. 10:1996;1.
7. Peterson G.L. Anal. Biochem. 100:1979;201.